Two thymidine kinases and one multisubstrate deoxyribonucleoside kinase salvage DNA precursors in Arabidopsis thaliana.
(2012) In The FEBS Journal 279(20). p.3889-3897- Abstract
- Deoxyribonucleotides are the building blocks of DNA and can be synthesized via de novo and salvage pathways. Deoxyribonucleoside kinases (dNKs) salvage deoxyribonucleosides by transfer of a phosphate group to the 5' of a deoxyribonucleoside. This salvage pathway is well characterized in mammals but in contrast little is known about how plants salvage deoxyribonucleosides. We show that during salvage, deoxyribonucleosides can be phosphorylated by extracts of Arabidopsis thaliana into corresponding mono-phosphate compounds with a surprising preference for purines over pyrimidines. Deoxyribonucleoside kinase activities were present in all tissues during all growth stages. In the A. thaliana genome we identified two types of genes that could... (More)
- Deoxyribonucleotides are the building blocks of DNA and can be synthesized via de novo and salvage pathways. Deoxyribonucleoside kinases (dNKs) salvage deoxyribonucleosides by transfer of a phosphate group to the 5' of a deoxyribonucleoside. This salvage pathway is well characterized in mammals but in contrast little is known about how plants salvage deoxyribonucleosides. We show that during salvage, deoxyribonucleosides can be phosphorylated by extracts of Arabidopsis thaliana into corresponding mono-phosphate compounds with a surprising preference for purines over pyrimidines. Deoxyribonucleoside kinase activities were present in all tissues during all growth stages. In the A. thaliana genome we identified two types of genes that could encode enzymes which are involved in the salvage of deoxyribonucleosides. Thymidine kinase activity was encoded by two thymidine kinase 1-like genes (AtTK1a and AtTK1b) and deoxyadenosine, deoxyguanosine and deoxycytidine kinase activities were encoded by a single AtdNK gene. T-DNA insertion lines of AtTK1a and AtTK1b mutant genes had normal growth, but AtTK1a AtTK1b double mutants died at an early stage, which indicates that AtTK1a and AtTK1b catalyze redundant reactions. Our results point out a crucial role for salvage of thymidine during early plant development. © 2012 The Authors Journal compilation © 2012 FEBS. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3047472
- author
- Clausen, Anders Ranegaard LU ; Girandon, Lenart ; Ali, Ashfaq LU ; Knecht, Wolfgang LU ; Rozpedowska, Elzbieta LU ; Sandrini, Michael LU ; Andreasson, Erik LU ; Munch-Petersen, Birgitte and Piskur, Jure LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- The FEBS Journal
- volume
- 279
- issue
- 20
- pages
- 3889 - 3897
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000309447400010
- pmid:22897443
- scopus:84867103682
- pmid:22897443
- ISSN
- 1742-464X
- DOI
- 10.1111/j.1742-4658.2012.08747.x
- language
- English
- LU publication?
- yes
- id
- e5e0cb50-4134-4241-936a-b36a2767b4d6 (old id 3047472)
- date added to LUP
- 2016-04-01 13:23:20
- date last changed
- 2022-01-27 18:57:34
@article{e5e0cb50-4134-4241-936a-b36a2767b4d6, abstract = {{Deoxyribonucleotides are the building blocks of DNA and can be synthesized via de novo and salvage pathways. Deoxyribonucleoside kinases (dNKs) salvage deoxyribonucleosides by transfer of a phosphate group to the 5' of a deoxyribonucleoside. This salvage pathway is well characterized in mammals but in contrast little is known about how plants salvage deoxyribonucleosides. We show that during salvage, deoxyribonucleosides can be phosphorylated by extracts of Arabidopsis thaliana into corresponding mono-phosphate compounds with a surprising preference for purines over pyrimidines. Deoxyribonucleoside kinase activities were present in all tissues during all growth stages. In the A. thaliana genome we identified two types of genes that could encode enzymes which are involved in the salvage of deoxyribonucleosides. Thymidine kinase activity was encoded by two thymidine kinase 1-like genes (AtTK1a and AtTK1b) and deoxyadenosine, deoxyguanosine and deoxycytidine kinase activities were encoded by a single AtdNK gene. T-DNA insertion lines of AtTK1a and AtTK1b mutant genes had normal growth, but AtTK1a AtTK1b double mutants died at an early stage, which indicates that AtTK1a and AtTK1b catalyze redundant reactions. Our results point out a crucial role for salvage of thymidine during early plant development. © 2012 The Authors Journal compilation © 2012 FEBS.}}, author = {{Clausen, Anders Ranegaard and Girandon, Lenart and Ali, Ashfaq and Knecht, Wolfgang and Rozpedowska, Elzbieta and Sandrini, Michael and Andreasson, Erik and Munch-Petersen, Birgitte and Piskur, Jure}}, issn = {{1742-464X}}, language = {{eng}}, number = {{20}}, pages = {{3889--3897}}, publisher = {{Wiley-Blackwell}}, series = {{The FEBS Journal}}, title = {{Two thymidine kinases and one multisubstrate deoxyribonucleoside kinase salvage DNA precursors in Arabidopsis thaliana.}}, url = {{http://dx.doi.org/10.1111/j.1742-4658.2012.08747.x}}, doi = {{10.1111/j.1742-4658.2012.08747.x}}, volume = {{279}}, year = {{2012}}, }