Histology-based blood leukocyte profiling reveals parallel Th2 and Th17 signatures in seasonal allergic rhinitis
(2022) In Acta Oto-Laryngologica 142(9-12). p.696-704- Abstract
BACKGROUND: Allergic rhinitis (AR), a common condition in the westernized world, is suggested to be more immunologically complex than the archetypical 'Th2' inflammation. New approaches are needed to decode this complexity.
AIMS/OBJECTIVES: In this study, we explored a novel histology-based analysis for circulating blood leukocyte profiling in 16 patients with seasonal AR outside and during the pollen season.
MATERIAL AND METHODS: Leukocytes were purified with minimal ex-vivo artefacts, embedded into agarose-paraffin pellets for immunohistochemistry-based immune cell profiling. Blood leukocyte mapping was performed.
RESULTS: Samples collected during the pollen season had statistically increased eosinophils,... (More)
BACKGROUND: Allergic rhinitis (AR), a common condition in the westernized world, is suggested to be more immunologically complex than the archetypical 'Th2' inflammation. New approaches are needed to decode this complexity.
AIMS/OBJECTIVES: In this study, we explored a novel histology-based analysis for circulating blood leukocyte profiling in 16 patients with seasonal AR outside and during the pollen season.
MATERIAL AND METHODS: Leukocytes were purified with minimal ex-vivo artefacts, embedded into agarose-paraffin pellets for immunohistochemistry-based immune cell profiling. Blood leukocyte mapping was performed.
RESULTS: Samples collected during the pollen season had statistically increased eosinophils, neutrophils, monocytes, and CD8+ T-lymphocytes compared to the off-season baseline. In contrast, no change was observed for CD20+ B-lymphocytes and CD3+ T-lymphocytes. Subclassification of CD4+ T-helper cells demonstrated a parallel and significant expansion of Th2 and Th17-cells during the pollen season, while Th1-cells remained unchanged. Whereas absolute basophils numbers were unaltered, the basophil markers GATA2 and CPA3 increased during the pollen season.
CONCLUSIONS AND SIGNIFICANCE: This study introduces a novel and applicable method for systemic immune cell screening and provides further evidence of complex and parallel Th2 and Th17-immune signatures in seasonal AR. It also forwards GATA2 and CPA3 as potential biomarkers for ongoing allergic inflammation.
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- author
- Siddhuraj, Premkumar LU ; Nordström, Franziska U LU ; Jogdand, Prajakta LU ; Elangovan, Gayathri ; Mori, Michiko LU ; Bjermer, Leif LU ; Greiff, Lennart LU and Erjefält, Jonas S LU
- organization
-
- Airway Inflammation and Immunology (research group)
- Otorhinolaryngology (Lund)
- Department of Experimental Medical Science
- Clinical Respiratory Medicine (research group)
- Respiratory Medicine, Allergology, and Palliative Medicine
- Head and Neck Cancer Research Group (research group)
- DCD transplantation of lungs (research group)
- publishing date
- 2022-12-23
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Acta Oto-Laryngologica
- volume
- 142
- issue
- 9-12
- pages
- 696 - 704
- publisher
- Taylor & Francis
- external identifiers
-
- scopus:85145060147
- pmid:36562632
- ISSN
- 1651-2251
- DOI
- 10.1080/00016489.2022.2155316
- language
- English
- LU publication?
- yes
- id
- e660634b-45d8-4cb7-a1e2-8f21ec7a35d6
- date added to LUP
- 2022-12-29 14:42:16
- date last changed
- 2024-04-16 22:17:18
@article{e660634b-45d8-4cb7-a1e2-8f21ec7a35d6,
abstract = {{<p>BACKGROUND: Allergic rhinitis (AR), a common condition in the westernized world, is suggested to be more immunologically complex than the archetypical 'Th2' inflammation. New approaches are needed to decode this complexity.</p><p>AIMS/OBJECTIVES: In this study, we explored a novel histology-based analysis for circulating blood leukocyte profiling in 16 patients with seasonal AR outside and during the pollen season.</p><p>MATERIAL AND METHODS: Leukocytes were purified with minimal ex-vivo artefacts, embedded into agarose-paraffin pellets for immunohistochemistry-based immune cell profiling. Blood leukocyte mapping was performed.</p><p>RESULTS: Samples collected during the pollen season had statistically increased eosinophils, neutrophils, monocytes, and CD8+ T-lymphocytes compared to the off-season baseline. In contrast, no change was observed for CD20+ B-lymphocytes and CD3+ T-lymphocytes. Subclassification of CD4+ T-helper cells demonstrated a parallel and significant expansion of Th2 and Th17-cells during the pollen season, while Th1-cells remained unchanged. Whereas absolute basophils numbers were unaltered, the basophil markers GATA2 and CPA3 increased during the pollen season.</p><p>CONCLUSIONS AND SIGNIFICANCE: This study introduces a novel and applicable method for systemic immune cell screening and provides further evidence of complex and parallel Th2 and Th17-immune signatures in seasonal AR. It also forwards GATA2 and CPA3 as potential biomarkers for ongoing allergic inflammation.</p>}},
author = {{Siddhuraj, Premkumar and Nordström, Franziska U and Jogdand, Prajakta and Elangovan, Gayathri and Mori, Michiko and Bjermer, Leif and Greiff, Lennart and Erjefält, Jonas S}},
issn = {{1651-2251}},
language = {{eng}},
month = {{12}},
number = {{9-12}},
pages = {{696--704}},
publisher = {{Taylor & Francis}},
series = {{Acta Oto-Laryngologica}},
title = {{Histology-based blood leukocyte profiling reveals parallel Th2 and Th17 signatures in seasonal allergic rhinitis}},
url = {{http://dx.doi.org/10.1080/00016489.2022.2155316}},
doi = {{10.1080/00016489.2022.2155316}},
volume = {{142}},
year = {{2022}},
}