Cystatin C properties crucial for uptake and inhibition of intracellular target enzymes.
(2013) In Journal of Biological Chemistry 288(23). p.17019-17029- Abstract
- To elucidate the molecular requirements for cancer cell internalization of the extracellular cysteine protease inhibitor cystatin C, 12 variants of the protein were produced and used for uptake experiments in MCF-7 cells. Variants with alterations in the cysteine cathepsin binding region ((Δ1-10)-, K5A-, R8G-, (R8G,L9G,V10G)-, (R8G,L9G,V10G, W106G)-, and W106G-cystatin C) were internalized to a very low extent compared to the wild-type inhibitor. Substitutions of N39 in the legumain binding region (N39K- and N39A-cystatin C) decreased the internalization and (R24A,R25A)-cystatin C, with substitutions of charged residues not involved in enzyme inhibition, was not taken up at all. Two variants, W106F- and K75A-cystatin C, showed that the... (More)
- To elucidate the molecular requirements for cancer cell internalization of the extracellular cysteine protease inhibitor cystatin C, 12 variants of the protein were produced and used for uptake experiments in MCF-7 cells. Variants with alterations in the cysteine cathepsin binding region ((Δ1-10)-, K5A-, R8G-, (R8G,L9G,V10G)-, (R8G,L9G,V10G, W106G)-, and W106G-cystatin C) were internalized to a very low extent compared to the wild-type inhibitor. Substitutions of N39 in the legumain binding region (N39K- and N39A-cystatin C) decreased the internalization and (R24A,R25A)-cystatin C, with substitutions of charged residues not involved in enzyme inhibition, was not taken up at all. Two variants, W106F- and K75A-cystatin C, showed that the internalization can be positively affected by engineering of the cystatin molecule. Microscopy revealed vesicular co-localization of internalized cystatin C with the lysosomal marker proteins cathepsin D and legumain. Activities of both cysteine cathepsins and legumain, possible target enzymes associated with cancer cell invasion and metastasis, were down-regulated in cell homogenates following cystatin C uptake. A positive effect on regulation of intracellular enzyme activity by a cystatin variant selected from uptake properties was illustrated by incubating cells with W106F-cystatin C. This resulted in more efficient down-regulation of intracellular legumain activity than when cells were incubated with wild-type cystatin C. Uptake experiments in prostate cancer cells corroborated that the cystatin C internalization is generally relevant and confirmed an increased uptake of W106F-cystatin C, in PC3 cells. Thus, intracellular cysteine proteases involved in cancer-promoting processes might be controled by cystatin uptake. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3805208
- author
- Wallin, Hanna LU ; Abrahamson, Magnus LU and Ekström, Ulf LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Biological Chemistry
- volume
- 288
- issue
- 23
- pages
- 17019 - 17029
- publisher
- American Society for Biochemistry and Molecular Biology
- external identifiers
-
- wos:000320378900073
- pmid:23629651
- scopus:84878759123
- pmid:23629651
- ISSN
- 1083-351X
- DOI
- 10.1074/jbc.M113.453449
- language
- English
- LU publication?
- yes
- id
- e66859d9-e6dd-43b5-a789-4e4b6f08600e (old id 3805208)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/23629651?dopt=Abstract
- date added to LUP
- 2016-04-01 09:59:05
- date last changed
- 2022-03-19 08:15:20
@article{e66859d9-e6dd-43b5-a789-4e4b6f08600e,
abstract = {{To elucidate the molecular requirements for cancer cell internalization of the extracellular cysteine protease inhibitor cystatin C, 12 variants of the protein were produced and used for uptake experiments in MCF-7 cells. Variants with alterations in the cysteine cathepsin binding region ((Δ1-10)-, K5A-, R8G-, (R8G,L9G,V10G)-, (R8G,L9G,V10G, W106G)-, and W106G-cystatin C) were internalized to a very low extent compared to the wild-type inhibitor. Substitutions of N39 in the legumain binding region (N39K- and N39A-cystatin C) decreased the internalization and (R24A,R25A)-cystatin C, with substitutions of charged residues not involved in enzyme inhibition, was not taken up at all. Two variants, W106F- and K75A-cystatin C, showed that the internalization can be positively affected by engineering of the cystatin molecule. Microscopy revealed vesicular co-localization of internalized cystatin C with the lysosomal marker proteins cathepsin D and legumain. Activities of both cysteine cathepsins and legumain, possible target enzymes associated with cancer cell invasion and metastasis, were down-regulated in cell homogenates following cystatin C uptake. A positive effect on regulation of intracellular enzyme activity by a cystatin variant selected from uptake properties was illustrated by incubating cells with W106F-cystatin C. This resulted in more efficient down-regulation of intracellular legumain activity than when cells were incubated with wild-type cystatin C. Uptake experiments in prostate cancer cells corroborated that the cystatin C internalization is generally relevant and confirmed an increased uptake of W106F-cystatin C, in PC3 cells. Thus, intracellular cysteine proteases involved in cancer-promoting processes might be controled by cystatin uptake.}},
author = {{Wallin, Hanna and Abrahamson, Magnus and Ekström, Ulf}},
issn = {{1083-351X}},
language = {{eng}},
number = {{23}},
pages = {{17019--17029}},
publisher = {{American Society for Biochemistry and Molecular Biology}},
series = {{Journal of Biological Chemistry}},
title = {{Cystatin C properties crucial for uptake and inhibition of intracellular target enzymes.}},
url = {{https://lup.lub.lu.se/search/files/1451378/4180004.pdf}},
doi = {{10.1074/jbc.M113.453449}},
volume = {{288}},
year = {{2013}},
}