Label-free separation of peripheral blood mononuclear cells from whole blood by gradient acoustic focusing
Alsved, Julia ; Rezayati Charan, Mahdi LU ; Ohlsson, Pelle LU
; Urbansky, Anke
LU
and Augustsson, Per
LU
(2024)
In Scientific Reports
14(1).
- Abstract
Efficient techniques for separating target cells from undiluted blood are necessary for various diagnostic and research applications. This paper presents acoustic focusing in dense media containing iodixanol to purify peripheral blood mononuclear cells (PBMCs) from whole blood in a label-free and flow-through format. If the blood is laminated or mixed with iodixanol solutions while passing through the resonant microchannel, all the components (fluids and cells) rearrange according to their acoustic impedances. Red blood cells (RBCs) have higher effective acoustic impedance than PBMCs. Therefore, they relocate to the pressure node despite the dense medium, while PBMCs stay near the channel walls due to their negative contrast factor... (More)
Efficient techniques for separating target cells from undiluted blood are necessary for various diagnostic and research applications. This paper presents acoustic focusing in dense media containing iodixanol to purify peripheral blood mononuclear cells (PBMCs) from whole blood in a label-free and flow-through format. If the blood is laminated or mixed with iodixanol solutions while passing through the resonant microchannel, all the components (fluids and cells) rearrange according to their acoustic impedances. Red blood cells (RBCs) have higher effective acoustic impedance than PBMCs. Therefore, they relocate to the pressure node despite the dense medium, while PBMCs stay near the channel walls due to their negative contrast factor relative to their surrounding medium. By modifying the medium and thus tuning the contrast factor of the cells, we enriched PBMCs relative to RBCs by a factor of 3600 to 11,000 and with a separation efficiency of 85%. That level of RBC depletion is higher than most other microfluidic methods and similar to that of density gradient centrifugation. The current acoustophoretic chip runs up to 20 µl/min undiluted whole blood and can be integrated with downstream analysis.
(Less)
- author
- Alsved, Julia
; Rezayati Charan, Mahdi
LU
; Ohlsson, Pelle
LU
; Urbansky, Anke
LU
and Augustsson, Per
LU
- organization
- publishing date
- 2024-04-16
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Leukocytes, Mononuclear, Cell Separation/methods, Triiodobenzoic Acids, Acoustics, Microfluidic Analytical Techniques/methods
- in
- Scientific Reports
- volume
- 14
- issue
- 1
- article number
- 8748
- pages
- 12 pages
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:38627566
- scopus:85190538112
- ISSN
- 2045-2322
- DOI
- 10.1038/s41598-024-59156-7
- language
- English
- LU publication?
- yes
- additional info
- © 2024. The Author(s).
- id
- e68663c2-6920-4075-8cf2-5f3e7214a4c8
- date added to LUP
- 2024-04-22 10:41:42
- date last changed
- 2024-06-18 08:53:13
@article{e68663c2-6920-4075-8cf2-5f3e7214a4c8,
abstract = {{<p>Efficient techniques for separating target cells from undiluted blood are necessary for various diagnostic and research applications. This paper presents acoustic focusing in dense media containing iodixanol to purify peripheral blood mononuclear cells (PBMCs) from whole blood in a label-free and flow-through format. If the blood is laminated or mixed with iodixanol solutions while passing through the resonant microchannel, all the components (fluids and cells) rearrange according to their acoustic impedances. Red blood cells (RBCs) have higher effective acoustic impedance than PBMCs. Therefore, they relocate to the pressure node despite the dense medium, while PBMCs stay near the channel walls due to their negative contrast factor relative to their surrounding medium. By modifying the medium and thus tuning the contrast factor of the cells, we enriched PBMCs relative to RBCs by a factor of 3600 to 11,000 and with a separation efficiency of 85%. That level of RBC depletion is higher than most other microfluidic methods and similar to that of density gradient centrifugation. The current acoustophoretic chip runs up to 20 µl/min undiluted whole blood and can be integrated with downstream analysis.</p>}},
author = {{Alsved, Julia and Rezayati Charan, Mahdi and Ohlsson, Pelle and Urbansky, Anke and Augustsson, Per}},
issn = {{2045-2322}},
keywords = {{Leukocytes, Mononuclear; Cell Separation/methods; Triiodobenzoic Acids; Acoustics; Microfluidic Analytical Techniques/methods}},
language = {{eng}},
month = {{04}},
number = {{1}},
publisher = {{Nature Publishing Group}},
series = {{Scientific Reports}},
title = {{Label-free separation of peripheral blood mononuclear cells from whole blood by gradient acoustic focusing}},
url = {{http://dx.doi.org/10.1038/s41598-024-59156-7}},
doi = {{10.1038/s41598-024-59156-7}},
volume = {{14}},
year = {{2024}},
}