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Purification and characterization of a protein binding to the SP6 κ promoter : A potential role for CArG-box binding factor-A in κ transcription

Bemark, Mats LU orcid ; Olsson, Henric ; Heinegård, Dick LU and Leanderson, Tomas LU (1998) In Journal of Biological Chemistry 273(30). p.18881-18890
Abstract

A protein interacting with an A-T-rich region that is a positive control element within the SP6 κ promoter was purified and identified as CArG-box binding factor-A. The purified protein was shown to interact specifically with the coding strand of single-stranded DNA and, with lower affinity, with double-stranded DNA. A mutation that inhibited binding of the protein to the A-T-rich region also aborted the transcriptional stimulatory effect of the region. Two Ets proteins, PU.1 and elf-1, that have previously been shown to bind to an adjacent DNA element were shown to physically interact with CArG- box binding factor.A. An antiserum raised against the protein recognized two different forms indicating either that different splice-forms of... (More)

A protein interacting with an A-T-rich region that is a positive control element within the SP6 κ promoter was purified and identified as CArG-box binding factor-A. The purified protein was shown to interact specifically with the coding strand of single-stranded DNA and, with lower affinity, with double-stranded DNA. A mutation that inhibited binding of the protein to the A-T-rich region also aborted the transcriptional stimulatory effect of the region. Two Ets proteins, PU.1 and elf-1, that have previously been shown to bind to an adjacent DNA element were shown to physically interact with CArG- box binding factor.A. An antiserum raised against the protein recognized two different forms indicating either that different splice-forms of CArG-box binding factor-A are expressed, or that the protein is subject to posttranslational modification.

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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Biological Chemistry
volume
273
issue
30
pages
18881 - 18890
publisher
American Society for Biochemistry and Molecular Biology
external identifiers
  • pmid:9668064
  • scopus:0032563096
ISSN
0021-9258
DOI
10.1074/jbc.273.30.18881
language
English
LU publication?
yes
id
e68a32da-f830-4928-b33a-e1756ca00fe5
date added to LUP
2023-11-28 10:21:34
date last changed
2024-02-09 10:19:17
@article{e68a32da-f830-4928-b33a-e1756ca00fe5,
  abstract     = {{<p>A protein interacting with an A-T-rich region that is a positive control element within the SP6 κ promoter was purified and identified as CArG-box binding factor-A. The purified protein was shown to interact specifically with the coding strand of single-stranded DNA and, with lower affinity, with double-stranded DNA. A mutation that inhibited binding of the protein to the A-T-rich region also aborted the transcriptional stimulatory effect of the region. Two Ets proteins, PU.1 and elf-1, that have previously been shown to bind to an adjacent DNA element were shown to physically interact with CArG- box binding factor.A. An antiserum raised against the protein recognized two different forms indicating either that different splice-forms of CArG-box binding factor-A are expressed, or that the protein is subject to posttranslational modification.</p>}},
  author       = {{Bemark, Mats and Olsson, Henric and Heinegård, Dick and Leanderson, Tomas}},
  issn         = {{0021-9258}},
  language     = {{eng}},
  month        = {{07}},
  number       = {{30}},
  pages        = {{18881--18890}},
  publisher    = {{American Society for Biochemistry and Molecular Biology}},
  series       = {{Journal of Biological Chemistry}},
  title        = {{Purification and characterization of a protein binding to the SP6 κ promoter : A potential role for CArG-box binding factor-A in κ transcription}},
  url          = {{http://dx.doi.org/10.1074/jbc.273.30.18881}},
  doi          = {{10.1074/jbc.273.30.18881}},
  volume       = {{273}},
  year         = {{1998}},
}