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Chitosan tubes of varying degrees of acetylation for bridging peripheral nerve defects

Haastert-Talini, Kirsten ; Geuna, Stefano ; Dahlin, Lars LU ; Meyer, Cora ; Stenberg, Lena LU ; Freier, Thomas ; Heimann, Claudia ; Barwig, Christina ; Pinto, Luis F. V. and Raimondo, Stefania , et al. (2013) In Biomaterials 34(38). p.9886-9904
Abstract
Biosynthetic nerve grafts are desired as alternative to autologous nerve grafts in peripheral nerve reconstruction. Artificial nerve conduits still have their limitations and are not widely accepted in the clinical setting. Here we report an analysis of fine-tuned chitosan tubes used to reconstruct 10 mm nerve defects in the adult rat. The chitosan tubes displayed low, medium and high degrees of acetylation (DAI: similar to 2%, DA: similar to 5%, DAIII: similar to 20%) and therefore different degradability and microenvironments for the regenerating nerve tissue. Short and long term investigations were performed demonstrating that the chitosan tubes allowed functional and morphological nerve regeneration similar to autologous nerve grafts.... (More)
Biosynthetic nerve grafts are desired as alternative to autologous nerve grafts in peripheral nerve reconstruction. Artificial nerve conduits still have their limitations and are not widely accepted in the clinical setting. Here we report an analysis of fine-tuned chitosan tubes used to reconstruct 10 mm nerve defects in the adult rat. The chitosan tubes displayed low, medium and high degrees of acetylation (DAI: similar to 2%, DA: similar to 5%, DAIII: similar to 20%) and therefore different degradability and microenvironments for the regenerating nerve tissue. Short and long term investigations were performed demonstrating that the chitosan tubes allowed functional and morphological nerve regeneration similar to autologous nerve grafts. Irrespective of the DA growth factor regulation demonstrated to be the same as in controls. Analyses of stereological parameters as well as the immunological tissue response at the implantation site and in the regenerated nerves, revealed that DAI and DAIII chitosan tubes displayed some limitations in the support of axonal regeneration and a high speed of degradation accompanied with low mechanical stability, respectively. The chitosan tubes combine several pre-requisites for a clinical acceptance and DAII chitosan tubes have to be judged as the most supportive for peripheral nerve regeneration. (C) 2013 The Authors. Published by Elsevier Ltd. All rights reserved. (Less)
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keywords
Chitosan, Nerve guide, Electrophysiology, Histomorphometry, Connective, tissue, Degradation
in
Biomaterials
volume
34
issue
38
pages
9886 - 9904
publisher
Elsevier
external identifiers
  • wos:000328094600016
  • scopus:84885374434
  • pmid:24050875
ISSN
1878-5905
DOI
10.1016/j.biomaterials.2013.08.074
language
English
LU publication?
yes
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e6cd2d05-e574-46a4-a2d5-81f98bfc0669 (old id 4272755)
date added to LUP
2016-04-01 10:21:38
date last changed
2020-08-02 03:15:32
@article{e6cd2d05-e574-46a4-a2d5-81f98bfc0669,
  abstract     = {Biosynthetic nerve grafts are desired as alternative to autologous nerve grafts in peripheral nerve reconstruction. Artificial nerve conduits still have their limitations and are not widely accepted in the clinical setting. Here we report an analysis of fine-tuned chitosan tubes used to reconstruct 10 mm nerve defects in the adult rat. The chitosan tubes displayed low, medium and high degrees of acetylation (DAI: similar to 2%, DA: similar to 5%, DAIII: similar to 20%) and therefore different degradability and microenvironments for the regenerating nerve tissue. Short and long term investigations were performed demonstrating that the chitosan tubes allowed functional and morphological nerve regeneration similar to autologous nerve grafts. Irrespective of the DA growth factor regulation demonstrated to be the same as in controls. Analyses of stereological parameters as well as the immunological tissue response at the implantation site and in the regenerated nerves, revealed that DAI and DAIII chitosan tubes displayed some limitations in the support of axonal regeneration and a high speed of degradation accompanied with low mechanical stability, respectively. The chitosan tubes combine several pre-requisites for a clinical acceptance and DAII chitosan tubes have to be judged as the most supportive for peripheral nerve regeneration. (C) 2013 The Authors. Published by Elsevier Ltd. All rights reserved.},
  author       = {Haastert-Talini, Kirsten and Geuna, Stefano and Dahlin, Lars and Meyer, Cora and Stenberg, Lena and Freier, Thomas and Heimann, Claudia and Barwig, Christina and Pinto, Luis F. V. and Raimondo, Stefania and Gambarotta, Giovanna and Samy, Silvina Ribeiro and Sousa, Nuno and Salgado, Antonio J. and Ratzka, Andreas and Wrobel, Sandra and Grothe, Claudia},
  issn         = {1878-5905},
  language     = {eng},
  number       = {38},
  pages        = {9886--9904},
  publisher    = {Elsevier},
  series       = {Biomaterials},
  title        = {Chitosan tubes of varying degrees of acetylation for bridging peripheral nerve defects},
  url          = {https://lup.lub.lu.se/search/ws/files/1780665/4611561.pdf},
  doi          = {10.1016/j.biomaterials.2013.08.074},
  volume       = {34},
  year         = {2013},
}