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Evaluation of electron mediators for the electromicrobial reduction of chloropyruvate by Proteus vulgaris cells

Andersson, Mats LU ; Holmberg, Hans and Adlercreutz, Patrick LU orcid (1997) In Enzyme and Microbial Technology 20(2). p.150-156
Abstract

Different electron mediators were evaluated for the electromicrobial reduction of chloropyruvate to chlorolactate. The reaction was catalyzed by a mediatol-dependent D-lactate dehydrogenase in whole cells of Proteus vulgaris and yielded D-(S)-chlorolactate with high optical purity (> 97% e.e.). The ideal mediatol should favor a high rate in the enzymatic reaction and a low rate in the nonenzymatic dehalogenation of the substrate. The latter reaction causes the formation of pyruvate and subsequently lactate. Viologens as mediators provided better yields than cobalt sepulchrate, safranine O, and an anthraquinone derivative. The best results were obtained with 1,1'-carbamoylmethylviologen as mediator.

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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Artificial electron mediators, D-chlorolactic acid, Electromicrobial reduction, Proteus vulgaris
in
Enzyme and Microbial Technology
volume
20
issue
2
pages
7 pages
publisher
Elsevier
external identifiers
  • scopus:0031081804
ISSN
0141-0229
DOI
10.1016/S0141-0229(96)00107-X
language
English
LU publication?
yes
id
e6d48bd4-a955-47c4-a0dc-4c596424eb32
date added to LUP
2019-06-20 16:31:29
date last changed
2022-01-31 22:13:27
@article{e6d48bd4-a955-47c4-a0dc-4c596424eb32,
  abstract     = {{<p>Different electron mediators were evaluated for the electromicrobial reduction of chloropyruvate to chlorolactate. The reaction was catalyzed by a mediatol-dependent D-lactate dehydrogenase in whole cells of Proteus vulgaris and yielded D-(S)-chlorolactate with high optical purity (&gt; 97% e.e.). The ideal mediatol should favor a high rate in the enzymatic reaction and a low rate in the nonenzymatic dehalogenation of the substrate. The latter reaction causes the formation of pyruvate and subsequently lactate. Viologens as mediators provided better yields than cobalt sepulchrate, safranine O, and an anthraquinone derivative. The best results were obtained with 1,1'-carbamoylmethylviologen as mediator.</p>}},
  author       = {{Andersson, Mats and Holmberg, Hans and Adlercreutz, Patrick}},
  issn         = {{0141-0229}},
  keywords     = {{Artificial electron mediators; D-chlorolactic acid; Electromicrobial reduction; Proteus vulgaris}},
  language     = {{eng}},
  month        = {{02}},
  number       = {{2}},
  pages        = {{150--156}},
  publisher    = {{Elsevier}},
  series       = {{Enzyme and Microbial Technology}},
  title        = {{Evaluation of electron mediators for the electromicrobial reduction of chloropyruvate by Proteus vulgaris cells}},
  url          = {{http://dx.doi.org/10.1016/S0141-0229(96)00107-X}},
  doi          = {{10.1016/S0141-0229(96)00107-X}},
  volume       = {{20}},
  year         = {{1997}},
}