Evaluation of electron mediators for the electromicrobial reduction of chloropyruvate by Proteus vulgaris cells
(1997) In Enzyme and Microbial Technology 20(2). p.150-156- Abstract
Different electron mediators were evaluated for the electromicrobial reduction of chloropyruvate to chlorolactate. The reaction was catalyzed by a mediatol-dependent D-lactate dehydrogenase in whole cells of Proteus vulgaris and yielded D-(S)-chlorolactate with high optical purity (> 97% e.e.). The ideal mediatol should favor a high rate in the enzymatic reaction and a low rate in the nonenzymatic dehalogenation of the substrate. The latter reaction causes the formation of pyruvate and subsequently lactate. Viologens as mediators provided better yields than cobalt sepulchrate, safranine O, and an anthraquinone derivative. The best results were obtained with 1,1'-carbamoylmethylviologen as mediator.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/e6d48bd4-a955-47c4-a0dc-4c596424eb32
- author
- Andersson, Mats LU ; Holmberg, Hans and Adlercreutz, Patrick LU
- organization
- publishing date
- 1997-02-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Artificial electron mediators, D-chlorolactic acid, Electromicrobial reduction, Proteus vulgaris
- in
- Enzyme and Microbial Technology
- volume
- 20
- issue
- 2
- pages
- 7 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0031081804
- ISSN
- 0141-0229
- DOI
- 10.1016/S0141-0229(96)00107-X
- language
- English
- LU publication?
- yes
- id
- e6d48bd4-a955-47c4-a0dc-4c596424eb32
- date added to LUP
- 2019-06-20 16:31:29
- date last changed
- 2022-01-31 22:13:27
@article{e6d48bd4-a955-47c4-a0dc-4c596424eb32, abstract = {{<p>Different electron mediators were evaluated for the electromicrobial reduction of chloropyruvate to chlorolactate. The reaction was catalyzed by a mediatol-dependent D-lactate dehydrogenase in whole cells of Proteus vulgaris and yielded D-(S)-chlorolactate with high optical purity (> 97% e.e.). The ideal mediatol should favor a high rate in the enzymatic reaction and a low rate in the nonenzymatic dehalogenation of the substrate. The latter reaction causes the formation of pyruvate and subsequently lactate. Viologens as mediators provided better yields than cobalt sepulchrate, safranine O, and an anthraquinone derivative. The best results were obtained with 1,1'-carbamoylmethylviologen as mediator.</p>}}, author = {{Andersson, Mats and Holmberg, Hans and Adlercreutz, Patrick}}, issn = {{0141-0229}}, keywords = {{Artificial electron mediators; D-chlorolactic acid; Electromicrobial reduction; Proteus vulgaris}}, language = {{eng}}, month = {{02}}, number = {{2}}, pages = {{150--156}}, publisher = {{Elsevier}}, series = {{Enzyme and Microbial Technology}}, title = {{Evaluation of electron mediators for the electromicrobial reduction of chloropyruvate by Proteus vulgaris cells}}, url = {{http://dx.doi.org/10.1016/S0141-0229(96)00107-X}}, doi = {{10.1016/S0141-0229(96)00107-X}}, volume = {{20}}, year = {{1997}}, }