C-13 incorporation into signature fatty acids as an assay for carbon allocation in arbuscular mycorrhiza
(2005) In Applied and Environmental Microbiology 71(5). p.2592-2599- Abstract
- The ubiquitous arbuscular mycorrhizal fungi consume significant amounts of plant assimilated C, but this C flow has been difficult to quantify. The neutral lipid fatty acid 16:1ω 5 is a quantitative signature for most arbuscular mycorrhizal fungi in roots and soil. We measured carbon transfer from four plant species to the arbuscular mycorrhizal fungus Glomus intraradices by estimating C-13 enrichment of 16:1ω 5 and compared it with C-13 enrichment of total root and mycelial C. Carbon allocation to mycelia was detected within 1 day in monoxenic arbuscular mycorrhizal root cultures labeled with [C-13] glucose. The 13 C enrichment of neutral lipid fatty acid 16:1ω 5 extracted from roots increased from 0.14% 1 day after labeling to 2.2% 7... (More)
- The ubiquitous arbuscular mycorrhizal fungi consume significant amounts of plant assimilated C, but this C flow has been difficult to quantify. The neutral lipid fatty acid 16:1ω 5 is a quantitative signature for most arbuscular mycorrhizal fungi in roots and soil. We measured carbon transfer from four plant species to the arbuscular mycorrhizal fungus Glomus intraradices by estimating C-13 enrichment of 16:1ω 5 and compared it with C-13 enrichment of total root and mycelial C. Carbon allocation to mycelia was detected within 1 day in monoxenic arbuscular mycorrhizal root cultures labeled with [C-13] glucose. The 13 C enrichment of neutral lipid fatty acid 16:1ω 5 extracted from roots increased from 0.14% 1 day after labeling to 2.2% 7 days after labeling. The colonized roots usually were more enriched for C-13 in the arbuscular mycorrhizal fungal neutral lipid fatty acid 16:1w5 than for the root specific neutral lipid fatty acid 18:2ω 6,9. We labeled plant assimilates by using (CO2)-C-13 in whole-plant experiments. The extraradical mycelium often was more enriched for C-13 than was the intraradical mycelium, suggesting rapid translocation of carbon to and more active growth by the extraradical mycelium. Since there was a good correlation between 13C enrichment in neutral lipid fatty acid 16:1ω 5 and total C-13 in extraradical mycelia in different systems (r(2) = 0.94), we propose that the total amount of labeled C in intraradical and extraradical mycelium can be calculated from the C-13 enrichment of 16:1ω 5. The method described enables evaluation of C flow from plants to arbuscular mycorrhizal fungi to be made without extraction, purification and identification of fungal mycelia. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/146797
- author
- Olsson, Pål Axel LU ; van Aarle, I M ; Gavito, M E ; Bengtson, Per LU and Bengtsson, Göran LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Applied and Environmental Microbiology
- volume
- 71
- issue
- 5
- pages
- 2592 - 2599
- publisher
- American Society for Microbiology
- external identifiers
-
- wos:000229105300050
- pmid:15870350
- scopus:18444375576
- ISSN
- 0099-2240
- DOI
- 10.1128/AEM.71.5.2592-2599.2005
- language
- English
- LU publication?
- yes
- id
- e6d8bf89-b292-4834-aa53-c60e5d999d0a (old id 146797)
- date added to LUP
- 2016-04-01 11:43:12
- date last changed
- 2024-01-07 17:58:42
@article{e6d8bf89-b292-4834-aa53-c60e5d999d0a, abstract = {{The ubiquitous arbuscular mycorrhizal fungi consume significant amounts of plant assimilated C, but this C flow has been difficult to quantify. The neutral lipid fatty acid 16:1ω 5 is a quantitative signature for most arbuscular mycorrhizal fungi in roots and soil. We measured carbon transfer from four plant species to the arbuscular mycorrhizal fungus Glomus intraradices by estimating C-13 enrichment of 16:1ω 5 and compared it with C-13 enrichment of total root and mycelial C. Carbon allocation to mycelia was detected within 1 day in monoxenic arbuscular mycorrhizal root cultures labeled with [C-13] glucose. The 13 C enrichment of neutral lipid fatty acid 16:1ω 5 extracted from roots increased from 0.14% 1 day after labeling to 2.2% 7 days after labeling. The colonized roots usually were more enriched for C-13 in the arbuscular mycorrhizal fungal neutral lipid fatty acid 16:1w5 than for the root specific neutral lipid fatty acid 18:2ω 6,9. We labeled plant assimilates by using (CO2)-C-13 in whole-plant experiments. The extraradical mycelium often was more enriched for C-13 than was the intraradical mycelium, suggesting rapid translocation of carbon to and more active growth by the extraradical mycelium. Since there was a good correlation between 13C enrichment in neutral lipid fatty acid 16:1ω 5 and total C-13 in extraradical mycelia in different systems (r(2) = 0.94), we propose that the total amount of labeled C in intraradical and extraradical mycelium can be calculated from the C-13 enrichment of 16:1ω 5. The method described enables evaluation of C flow from plants to arbuscular mycorrhizal fungi to be made without extraction, purification and identification of fungal mycelia.}}, author = {{Olsson, Pål Axel and van Aarle, I M and Gavito, M E and Bengtson, Per and Bengtsson, Göran}}, issn = {{0099-2240}}, language = {{eng}}, number = {{5}}, pages = {{2592--2599}}, publisher = {{American Society for Microbiology}}, series = {{Applied and Environmental Microbiology}}, title = {{C-13 incorporation into signature fatty acids as an assay for carbon allocation in arbuscular mycorrhiza}}, url = {{http://dx.doi.org/10.1128/AEM.71.5.2592-2599.2005}}, doi = {{10.1128/AEM.71.5.2592-2599.2005}}, volume = {{71}}, year = {{2005}}, }