Mathematical modeling and statistical analysis of calcium-regulated insulin granule exocytosis in beta-cells from mice and humans.
Pedersen, Morten Gram LU ; Cortese, Giuliana and Eliasson, Lena LU
(2011)
In Progress in Biophysics and Molecular Biology
107(2).
p.257-264
- Abstract
- Insulin is released from pancreatic beta-cells as a result of Ca(2+)-evoked exocytosis of dense-core granules. Secretion is biphasic, which has been suggested to correspond to the release of different granule pools. Here we review and carefully reanalyze previously published patch-clamp data on depolarization-evoked Ca(2+)-currents and corresponding capacitance measurements. Using a statistical mixed-effects model, we show that the data indicate that pool depletion is negligible in response to short depolarizations in mouse beta-cells. We then review mathematical models of granule dynamics and exocytosis in rodent beta-cells and present a mathematical description of Ca(2+)-evoked exocytosis in human beta-cells, which show clear differences... (More)
- Insulin is released from pancreatic beta-cells as a result of Ca(2+)-evoked exocytosis of dense-core granules. Secretion is biphasic, which has been suggested to correspond to the release of different granule pools. Here we review and carefully reanalyze previously published patch-clamp data on depolarization-evoked Ca(2+)-currents and corresponding capacitance measurements. Using a statistical mixed-effects model, we show that the data indicate that pool depletion is negligible in response to short depolarizations in mouse beta-cells. We then review mathematical models of granule dynamics and exocytosis in rodent beta-cells and present a mathematical description of Ca(2+)-evoked exocytosis in human beta-cells, which show clear differences to their rodent counterparts. The model suggests that L- and P/Q-type Ca(2+)-channels are involved to a similar degree in exocytosis during electrical activity in human beta-cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2151103
- author
- Pedersen, Morten Gram
LU
; Cortese, Giuliana
and Eliasson, Lena
LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Progress in Biophysics and Molecular Biology
- volume
- 107
- issue
- 2
- pages
- 257 - 264
- publisher
- Elsevier
- external identifiers
-
- wos:000297489800006
- pmid:21839108
- scopus:82455199164
- pmid:21839108
- ISSN
- 1873-1732
- DOI
- 10.1016/j.pbiomolbio.2011.07.012
- language
- English
- LU publication?
- yes
- id
- e76f9bb5-4bfd-441e-b454-af132d72df21 (old id 2151103)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21839108?dopt=Abstract
- date added to LUP
- 2016-04-01 10:15:40
- date last changed
- 2022-02-17 08:21:42
@article{e76f9bb5-4bfd-441e-b454-af132d72df21,
abstract = {{Insulin is released from pancreatic beta-cells as a result of Ca(2+)-evoked exocytosis of dense-core granules. Secretion is biphasic, which has been suggested to correspond to the release of different granule pools. Here we review and carefully reanalyze previously published patch-clamp data on depolarization-evoked Ca(2+)-currents and corresponding capacitance measurements. Using a statistical mixed-effects model, we show that the data indicate that pool depletion is negligible in response to short depolarizations in mouse beta-cells. We then review mathematical models of granule dynamics and exocytosis in rodent beta-cells and present a mathematical description of Ca(2+)-evoked exocytosis in human beta-cells, which show clear differences to their rodent counterparts. The model suggests that L- and P/Q-type Ca(2+)-channels are involved to a similar degree in exocytosis during electrical activity in human beta-cells.}},
author = {{Pedersen, Morten Gram and Cortese, Giuliana and Eliasson, Lena}},
issn = {{1873-1732}},
language = {{eng}},
number = {{2}},
pages = {{257--264}},
publisher = {{Elsevier}},
series = {{Progress in Biophysics and Molecular Biology}},
title = {{Mathematical modeling and statistical analysis of calcium-regulated insulin granule exocytosis in beta-cells from mice and humans.}},
url = {{https://lup.lub.lu.se/search/files/1693666/2342428.pdf}},
doi = {{10.1016/j.pbiomolbio.2011.07.012}},
volume = {{107}},
year = {{2011}},
}