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Convergence of chromogranin and amyloid metabolism in the brain.

Johansson, Per LU ; Mattsson, Niklas LU ; Hansson, Oskar LU ; Wallin, A.; Johansson, J.-O.; Andreasson, U.; Andersen, O; Haghighi, S.; Olsson, M. and Stridsberg, M, et al. (2010) Alzheimer's Association International Conference on Alzheimer's Disease 2010 In Alzheimer's & Dementia 6(4). p.511-511
Abstract (Swedish)
Background: Much is unknown regarding the regulation of amyloid precursor protein (APP) processing in the human central nervous system. It has been hypothesized that amyloidogenic APP-processing preferentially occurs in the regulated secretory pathway of neurons. To test this hypothesis we looked for correlations of APP-derived molecules in CSF with chromogranin (Cg) derived peptides, representing the regulated secretion. Methods: Patients with Alzheimer's disease (AD, N = 32), multiple sclerosis (MS, N = 50) and healthy controls (N = 70) were enrolled. CSF was analyzed for the amyloid peptides Aβ1-42, Aβx-42, Aβx-40, Aβx-38, α-cleaved soluble APP (α-sAPP), β-cleaved soluble APP (β-sAPP), and peptides derived from CgB and SgII... (More)
Background: Much is unknown regarding the regulation of amyloid precursor protein (APP) processing in the human central nervous system. It has been hypothesized that amyloidogenic APP-processing preferentially occurs in the regulated secretory pathway of neurons. To test this hypothesis we looked for correlations of APP-derived molecules in CSF with chromogranin (Cg) derived peptides, representing the regulated secretion. Methods: Patients with Alzheimer's disease (AD, N = 32), multiple sclerosis (MS, N = 50) and healthy controls (N = 70) were enrolled. CSF was analyzed for the amyloid peptides Aβ1-42, Aβx-42, Aβx-40, Aβx-38, α-cleaved soluble APP (α-sAPP), β-cleaved soluble APP (β-sAPP), and peptides derived from CgB and SgII (Secretogranin-II, CgC). We investigated CSF levels of the protease BACE1, which processes APP into Aβ, in relation to Cg-levels. Finally, we measured Cg levels in cell media from untreated and BACE1-inhibited SH-SY5Y human neuroblastoma cells. Results: CSF Cg levels correlated to sAPP and Aβ peptides in AD, MS and controls, and to CSF BACE1. Cell medium from BACE1-inhibited cells had decreased CgB levels. Conclusions: These results suggest that a large part of APP in the human central nervous system is processed in the regulated secretory pathway of neurons. (Less)
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published
subject
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Alzheimer's & Dementia
volume
6
issue
4
pages
511 - 511
publisher
Elsevier
conference name
Alzheimer's Association International Conference on Alzheimer's Disease 2010
ISSN
1552-5279
DOI
10.1016/j.jalz.2010.05.1705
language
English
LU publication?
yes
id
e78cf298-f013-48de-88f5-d6679d7cb49f
date added to LUP
2016-12-02 20:50:20
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2018-05-29 11:33:09
@misc{e78cf298-f013-48de-88f5-d6679d7cb49f,
  abstract     = {Background: Much is unknown regarding the regulation of amyloid precursor protein (APP) processing in the human central nervous system. It has been hypothesized that amyloidogenic APP-processing preferentially occurs in the regulated secretory pathway of neurons. To test this hypothesis we looked for correlations of APP-derived molecules in CSF with chromogranin (Cg) derived peptides, representing the regulated secretion. Methods: Patients with Alzheimer's disease (AD, N = 32), multiple sclerosis (MS, N = 50) and healthy controls (N = 70) were enrolled. CSF was analyzed for the amyloid peptides Aβ1-42, Aβx-42, Aβx-40, Aβx-38, α-cleaved soluble APP (α-sAPP), β-cleaved soluble APP (β-sAPP), and peptides derived from CgB and SgII (Secretogranin-II, CgC). We investigated CSF levels of the protease BACE1, which processes APP into Aβ, in relation to Cg-levels. Finally, we measured Cg levels in cell media from untreated and BACE1-inhibited SH-SY5Y human neuroblastoma cells. Results: CSF Cg levels correlated to sAPP and Aβ peptides in AD, MS and controls, and to CSF BACE1. Cell medium from BACE1-inhibited cells had decreased CgB levels. Conclusions: These results suggest that a large part of APP in the human central nervous system is processed in the regulated secretory pathway of neurons.},
  author       = {Johansson, Per and Mattsson, Niklas and Hansson, Oskar and Wallin, A. and Johansson, J.-O. and Andreasson, U. and Andersen, O and Haghighi, S. and Olsson, M. and Stridsberg, M and Svensson, J. and Blennow, K. and Zetterberg, H.},
  issn         = {1552-5279},
  language     = {eng},
  note         = {Conference Abstract},
  number       = {4},
  pages        = {511--511},
  publisher    = {Elsevier},
  series       = {Alzheimer's & Dementia},
  title        = {Convergence of chromogranin and amyloid metabolism in the brain.},
  url          = {http://dx.doi.org/10.1016/j.jalz.2010.05.1705},
  volume       = {6},
  year         = {2010},
}