Convergence of chromogranin and amyloid metabolism in the brain.
Johansson, Per LU ; Mattsson, Niklas LU
; Hansson, Oskar
LU
; Wallin, A.
; Johansson, J.-O.
; Andreasson, U.
; Andersen, O
; Haghighi, S.
; Olsson, M.
and Stridsberg, M
, et al.
(2010)
Alzheimer's Association International Conference on Alzheimer's Disease 2010
In Alzheimer's & Dementia
6(4).
p.511-511
- Abstract
- Background: Much is unknown regarding the regulation of amyloid precursor protein (APP) processing in the human central nervous system. It has been hypothesized that amyloidogenic APP-processing preferentially occurs in the regulated secretory pathway of neurons. To test this hypothesis we looked for correlations of APP-derived molecules in CSF with chromogranin (Cg) derived peptides, representing the regulated secretion. Methods: Patients with Alzheimer's disease (AD, N = 32), multiple sclerosis (MS, N = 50) and healthy controls (N = 70) were enrolled. CSF was analyzed for the amyloid peptides Aβ1-42, Aβx-42, Aβx-40, Aβx-38, α-cleaved soluble APP (α-sAPP), β-cleaved soluble APP (β-sAPP), and peptides derived from CgB and SgII... (More)
- Background: Much is unknown regarding the regulation of amyloid precursor protein (APP) processing in the human central nervous system. It has been hypothesized that amyloidogenic APP-processing preferentially occurs in the regulated secretory pathway of neurons. To test this hypothesis we looked for correlations of APP-derived molecules in CSF with chromogranin (Cg) derived peptides, representing the regulated secretion. Methods: Patients with Alzheimer's disease (AD, N = 32), multiple sclerosis (MS, N = 50) and healthy controls (N = 70) were enrolled. CSF was analyzed for the amyloid peptides Aβ1-42, Aβx-42, Aβx-40, Aβx-38, α-cleaved soluble APP (α-sAPP), β-cleaved soluble APP (β-sAPP), and peptides derived from CgB and SgII (Secretogranin-II, CgC). We investigated CSF levels of the protease BACE1, which processes APP into Aβ, in relation to Cg-levels. Finally, we measured Cg levels in cell media from untreated and BACE1-inhibited SH-SY5Y human neuroblastoma cells. Results: CSF Cg levels correlated to sAPP and Aβ peptides in AD, MS and controls, and to CSF BACE1. Cell medium from BACE1-inhibited cells had decreased CgB levels. Conclusions: These results suggest that a large part of APP in the human central nervous system is processed in the regulated secretory pathway of neurons. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/e78cf298-f013-48de-88f5-d6679d7cb49f
- author
- Johansson, Per
LU
; Mattsson, Niklas
LU
; Hansson, Oskar
LU
; Wallin, A.
; Johansson, J.-O.
; Andreasson, U.
; Andersen, O
; Haghighi, S.
; Olsson, M.
and Stridsberg, M
, et al. (More)
- Johansson, Per
LU
; Mattsson, Niklas
LU
; Hansson, Oskar
LU
; Wallin, A.
; Johansson, J.-O.
; Andreasson, U.
; Andersen, O
; Haghighi, S.
; Olsson, M.
; Stridsberg, M
; Svensson, J.
; Blennow, K.
and Zetterberg, H.
(Less)
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Alzheimer's & Dementia
- volume
- 6
- issue
- 4
- pages
- 511 - 511
- publisher
- Wiley
- conference name
- Alzheimer's Association International Conference on Alzheimer's Disease 2010
- conference location
- Honolulu, United States
- conference dates
- 2010-07-10 - 2010-07-15
- ISSN
- 1552-5279
- DOI
- 10.1016/j.jalz.2010.05.1705
- project
- Endocrine and diagnostic aspects of cognitive impairment
- language
- English
- LU publication?
- yes
- id
- e78cf298-f013-48de-88f5-d6679d7cb49f
- date added to LUP
- 2016-12-02 20:50:20
- date last changed
- 2023-04-18 17:53:03
@misc{e78cf298-f013-48de-88f5-d6679d7cb49f,
abstract = {{Background: Much is unknown regarding the regulation of amyloid precursor protein (APP) processing in the human central nervous system. It has been hypothesized that amyloidogenic APP-processing preferentially occurs in the regulated secretory pathway of neurons. To test this hypothesis we looked for correlations of APP-derived molecules in CSF with chromogranin (Cg) derived peptides, representing the regulated secretion. Methods: Patients with Alzheimer's disease (AD, N = 32), multiple sclerosis (MS, N = 50) and healthy controls (N = 70) were enrolled. CSF was analyzed for the amyloid peptides Aβ1-42, Aβx-42, Aβx-40, Aβx-38, α-cleaved soluble APP (α-sAPP), β-cleaved soluble APP (β-sAPP), and peptides derived from CgB and SgII (Secretogranin-II, CgC). We investigated CSF levels of the protease BACE1, which processes APP into Aβ, in relation to Cg-levels. Finally, we measured Cg levels in cell media from untreated and BACE1-inhibited SH-SY5Y human neuroblastoma cells. Results: CSF Cg levels correlated to sAPP and Aβ peptides in AD, MS and controls, and to CSF BACE1. Cell medium from BACE1-inhibited cells had decreased CgB levels. Conclusions: These results suggest that a large part of APP in the human central nervous system is processed in the regulated secretory pathway of neurons.}},
author = {{Johansson, Per and Mattsson, Niklas and Hansson, Oskar and Wallin, A. and Johansson, J.-O. and Andreasson, U. and Andersen, O and Haghighi, S. and Olsson, M. and Stridsberg, M and Svensson, J. and Blennow, K. and Zetterberg, H.}},
issn = {{1552-5279}},
language = {{eng}},
note = {{Conference Abstract}},
number = {{4}},
pages = {{511--511}},
publisher = {{Wiley}},
series = {{Alzheimer's & Dementia}},
title = {{Convergence of chromogranin and amyloid metabolism in the brain.}},
url = {{http://dx.doi.org/10.1016/j.jalz.2010.05.1705}},
doi = {{10.1016/j.jalz.2010.05.1705}},
volume = {{6}},
year = {{2010}},
}