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Signal enhancement in supercritical fluid chromatography-diode-array detection with multiple injection

Sun, Mingzhe LU ; Turner, Charlotta LU and Sandahl, Margareta LU (2019) In Journal of Separation Science 42(24). p.3727-3737
Abstract

To circumvent the detrimental effects of large-volume injection with fixed-loop injector in modern supercritical fluid chromatography, the feasibility of performing multiple injection was investigated. By accumulating analytes from a certain number of continual small-volume injections, compounds can be concentrated on the column head, and this leads to signal enhancement compared with a single injection. The signal to noise enhancement of different compounds appeared to be associated with their retention on different stationary phases and with type of sample diluent. The diethylamine column gave the best signal to noise enhancement when acetonitrile was used as sample diluent and the 2-picolylamine column showed the best overall... (More)

To circumvent the detrimental effects of large-volume injection with fixed-loop injector in modern supercritical fluid chromatography, the feasibility of performing multiple injection was investigated. By accumulating analytes from a certain number of continual small-volume injections, compounds can be concentrated on the column head, and this leads to signal enhancement compared with a single injection. The signal to noise enhancement of different compounds appeared to be associated with their retention on different stationary phases and with type of sample diluent. The diethylamine column gave the best signal to noise enhancement when acetonitrile was used as sample diluent and the 2-picolylamine column showed the best overall performance with water as the sample diluent. The advantage of multiple injection over one-time large-volume injection was proven with sulfanilamide, with both acetonitrile and water as sample diluents. The multiple injection approach exhibited comparable within- and between-day precision of retention time and peak area with those of single injections. The potential of the multiple injection approach was demonstrated in the analysis of sulfanilamide-spiked honey extract and diclofenac-spiked ground water sample. The limitations of this approach were also discussed.

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Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
large injection volume, multiple injection, signal enhancement, supercritical fluid chromatography
in
Journal of Separation Science
volume
42
issue
24
pages
3727 - 3737
publisher
John Wiley & Sons
external identifiers
  • pmid:31625267
  • scopus:85075524824
ISSN
1615-9306
DOI
10.1002/jssc.201900614
language
English
LU publication?
yes
id
e82f5dbc-c710-48de-a567-4886d3f35db8
date added to LUP
2019-12-09 10:14:52
date last changed
2020-01-14 11:34:24
@article{e82f5dbc-c710-48de-a567-4886d3f35db8,
  abstract     = {<p>To circumvent the detrimental effects of large-volume injection with fixed-loop injector in modern supercritical fluid chromatography, the feasibility of performing multiple injection was investigated. By accumulating analytes from a certain number of continual small-volume injections, compounds can be concentrated on the column head, and this leads to signal enhancement compared with a single injection. The signal to noise enhancement of different compounds appeared to be associated with their retention on different stationary phases and with type of sample diluent. The diethylamine column gave the best signal to noise enhancement when acetonitrile was used as sample diluent and the 2-picolylamine column showed the best overall performance with water as the sample diluent. The advantage of multiple injection over one-time large-volume injection was proven with sulfanilamide, with both acetonitrile and water as sample diluents. The multiple injection approach exhibited comparable within- and between-day precision of retention time and peak area with those of single injections. The potential of the multiple injection approach was demonstrated in the analysis of sulfanilamide-spiked honey extract and diclofenac-spiked ground water sample. The limitations of this approach were also discussed.</p>},
  author       = {Sun, Mingzhe and Turner, Charlotta and Sandahl, Margareta},
  issn         = {1615-9306},
  language     = {eng},
  month        = {10},
  number       = {24},
  pages        = {3727--3737},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Separation Science},
  title        = {Signal enhancement in supercritical fluid chromatography-diode-array detection with multiple injection},
  url          = {http://dx.doi.org/10.1002/jssc.201900614},
  doi          = {10.1002/jssc.201900614},
  volume       = {42},
  year         = {2019},
}