The expression of myosin heavy chain (MHC) genes in human skeletal muscle is related to metabolic characteristics involved in the pathogenesis of type 2 diabetes.
Olsson, Anders H LU ; Rönn, Tina LU ; Elgzyri, Tarq LU ; Hansson, Ola LU
; Eriksson, Karl-Fredrik
LU
; Groop, Leif
LU
; Vaag, Allan
LU
; Poulsen, Pernille
and Ling, Charlotte
LU
(2011)
In Molecular Genetics and Metabolism
103.
p.275-281
- Abstract
- Type 2 diabetes patients exhibit a reduction in oxidative muscle fibres and an increase in glycolytic muscle fibres. In this study, we investigated whether both genetic and non-genetic factors influence the mRNA expression levels of three myosin heavy chain (MHC) genes represented in different fibre types. Specifically, we examined the MHC7 (slow-twitch oxidative fibre), MHCIIa (fast-twitch oxidative fibre) and MHCIIx/d (fast-twitch glycolytic fibre) genes in human skeletal muscle. We further investigated the use of MHC mRNA expression as a proxy to determine fibre-type composition, as measured by traditional ATP staining. Two cohorts of age-matched Swedish men were studied to determine the relationship of muscle mRNA expression of MHC7,... (More)
- Type 2 diabetes patients exhibit a reduction in oxidative muscle fibres and an increase in glycolytic muscle fibres. In this study, we investigated whether both genetic and non-genetic factors influence the mRNA expression levels of three myosin heavy chain (MHC) genes represented in different fibre types. Specifically, we examined the MHC7 (slow-twitch oxidative fibre), MHCIIa (fast-twitch oxidative fibre) and MHCIIx/d (fast-twitch glycolytic fibre) genes in human skeletal muscle. We further investigated the use of MHC mRNA expression as a proxy to determine fibre-type composition, as measured by traditional ATP staining. Two cohorts of age-matched Swedish men were studied to determine the relationship of muscle mRNA expression of MHC7, MHCIIa, and MHCIIx/d with muscle fibre composition. A classical twin approach, including young and elderly Danish twin pairs, was utilised to examine if differences in expression levels were due to genetic or environmental factors. Although MHCIIx/d mRNA expression correlated positively with the level of type IIx/d muscle fibres in the two cohorts (P<0.05), a relatively low magnitude of correlation suggests that mRNA does not fully correlate with fibre-type composition. Heritability estimates and genetic analysis suggest that the levels of MHC7, MHCIIa and MHCIIx/d expression are primarily under non-genetic influence, and MHCIIa indicated an age-related decline. PGC-1α exhibited a positive relationship with the expression of all three MHC genes (P<0.05); meanwhile, PGC-1β related positively with MHCIIa expression and negatively with MHCIIx/d expression (P<0.05). While MHCIIa expression related positively with insulin-stimulated glucose uptake (P<0.01), MHCIIx/d expression related negatively with insulin-stimulated glucose uptake (P<0.05). Our findings suggest that the expression levels of the MHC genes are associated with age and both PGC-1α and PGC-1β and indicate that the MHC genes may to some extent be used to determine fibre-type composition in human skeletal muscle. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1937408
- author
- Olsson, Anders H
LU
; Rönn, Tina
LU
; Elgzyri, Tarq
LU
; Hansson, Ola
LU
; Eriksson, Karl-Fredrik
LU
; Groop, Leif
LU
; Vaag, Allan
LU
; Poulsen, Pernille
and Ling, Charlotte
LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Molecular Genetics and Metabolism
- volume
- 103
- pages
- 275 - 281
- publisher
- Elsevier
- external identifiers
-
- wos:000291919500011
- pmid:21470888
- scopus:79958030339
- pmid:21470888
- ISSN
- 1096-7192
- DOI
- 10.1016/j.ymgme.2011.03.017
- language
- English
- LU publication?
- yes
- id
- e8cbd6f3-90fd-4288-b5a9-d1cf2ab1d997 (old id 1937408)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21470888?dopt=Abstract
- date added to LUP
- 2016-04-04 08:32:09
- date last changed
- 2024-05-11 23:36:51
@article{e8cbd6f3-90fd-4288-b5a9-d1cf2ab1d997,
abstract = {{Type 2 diabetes patients exhibit a reduction in oxidative muscle fibres and an increase in glycolytic muscle fibres. In this study, we investigated whether both genetic and non-genetic factors influence the mRNA expression levels of three myosin heavy chain (MHC) genes represented in different fibre types. Specifically, we examined the MHC7 (slow-twitch oxidative fibre), MHCIIa (fast-twitch oxidative fibre) and MHCIIx/d (fast-twitch glycolytic fibre) genes in human skeletal muscle. We further investigated the use of MHC mRNA expression as a proxy to determine fibre-type composition, as measured by traditional ATP staining. Two cohorts of age-matched Swedish men were studied to determine the relationship of muscle mRNA expression of MHC7, MHCIIa, and MHCIIx/d with muscle fibre composition. A classical twin approach, including young and elderly Danish twin pairs, was utilised to examine if differences in expression levels were due to genetic or environmental factors. Although MHCIIx/d mRNA expression correlated positively with the level of type IIx/d muscle fibres in the two cohorts (P<0.05), a relatively low magnitude of correlation suggests that mRNA does not fully correlate with fibre-type composition. Heritability estimates and genetic analysis suggest that the levels of MHC7, MHCIIa and MHCIIx/d expression are primarily under non-genetic influence, and MHCIIa indicated an age-related decline. PGC-1α exhibited a positive relationship with the expression of all three MHC genes (P<0.05); meanwhile, PGC-1β related positively with MHCIIa expression and negatively with MHCIIx/d expression (P<0.05). While MHCIIa expression related positively with insulin-stimulated glucose uptake (P<0.01), MHCIIx/d expression related negatively with insulin-stimulated glucose uptake (P<0.05). Our findings suggest that the expression levels of the MHC genes are associated with age and both PGC-1α and PGC-1β and indicate that the MHC genes may to some extent be used to determine fibre-type composition in human skeletal muscle.}},
author = {{Olsson, Anders H and Rönn, Tina and Elgzyri, Tarq and Hansson, Ola and Eriksson, Karl-Fredrik and Groop, Leif and Vaag, Allan and Poulsen, Pernille and Ling, Charlotte}},
issn = {{1096-7192}},
language = {{eng}},
pages = {{275--281}},
publisher = {{Elsevier}},
series = {{Molecular Genetics and Metabolism}},
title = {{The expression of myosin heavy chain (MHC) genes in human skeletal muscle is related to metabolic characteristics involved in the pathogenesis of type 2 diabetes.}},
url = {{http://dx.doi.org/10.1016/j.ymgme.2011.03.017}},
doi = {{10.1016/j.ymgme.2011.03.017}},
volume = {{103}},
year = {{2011}},
}