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Neurite outgrowth in cultured mouse pelvic ganglia - Effects of neurotrophins and bladder tissue

Ekman, Mari LU ; Zhu, Baoyi LU ; Swärd, Karl LU and Uvelius, Bengt LU (2017) In Autonomic Neuroscience: Basic and Clinical 205. p.41-49
Abstract

Neurotrophic factors regulate survival and growth of neurons. The urinary bladder is innervated via both sympathetic and parasympathetic neurons located in the major pelvic ganglion. The aim of the present study was to characterize the effects of the neurotrophins nerve growth factor (NGF), brain derived neurotrophic factor (BDNF) and neurotrophin 3 (NT-3) on the sprouting rate of sympathetic and parasympathetic neurites from the female mouse ganglion. The pelvic ganglion was dissected out and attached to a petri dish and cultured in vitro. All three factors (BDNF, NT-3 and NGF) stimulated neurite outgrowth of both sympathetic and parasympathetic neurites although BDNF and NT-3 had a higher stimulatory effect on parasympathetic ganglion... (More)

Neurotrophic factors regulate survival and growth of neurons. The urinary bladder is innervated via both sympathetic and parasympathetic neurons located in the major pelvic ganglion. The aim of the present study was to characterize the effects of the neurotrophins nerve growth factor (NGF), brain derived neurotrophic factor (BDNF) and neurotrophin 3 (NT-3) on the sprouting rate of sympathetic and parasympathetic neurites from the female mouse ganglion. The pelvic ganglion was dissected out and attached to a petri dish and cultured in vitro. All three factors (BDNF, NT-3 and NGF) stimulated neurite outgrowth of both sympathetic and parasympathetic neurites although BDNF and NT-3 had a higher stimulatory effect on parasympathetic ganglion cells. The neurotrophin receptors TrkA, TrkB and TrkC were all expressed in neurons of the ganglia. Co-culture of ganglia with urinary bladder tissue, but not diaphragm tissue, increased the sprouting rate of neurites. Active forms of BDNF and NT-3 were detected in urinary bladder tissue using western blotting whereas tissue from the diaphragm expressed NGF. Neurite outgrowth from the pelvic ganglion was inhibited by a TrkB receptor antagonist. We therefore suggest that the urinary bladder releases trophic factors, including BDNF and NT-3, which regulate neurite outgrowth via activation of neuronal Trk-receptors. These findings could influence future strategies for developing pharmaceuticals to improve re-innervation due to bladder pathologies.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
BNDF, NGF, NT-3, Parasympathetic, Sympathetic, Trk receptors
in
Autonomic Neuroscience: Basic and Clinical
volume
205
pages
41 - 49
publisher
Elsevier
external identifiers
  • scopus:85015985503
  • wos:000405047600006
ISSN
1566-0702
DOI
10.1016/j.autneu.2017.03.004
language
English
LU publication?
yes
id
e971804f-e787-4a68-8e77-d7d931c44241
date added to LUP
2017-04-19 15:00:38
date last changed
2017-09-18 13:32:44
@article{e971804f-e787-4a68-8e77-d7d931c44241,
  abstract     = {<p>Neurotrophic factors regulate survival and growth of neurons. The urinary bladder is innervated via both sympathetic and parasympathetic neurons located in the major pelvic ganglion. The aim of the present study was to characterize the effects of the neurotrophins nerve growth factor (NGF), brain derived neurotrophic factor (BDNF) and neurotrophin 3 (NT-3) on the sprouting rate of sympathetic and parasympathetic neurites from the female mouse ganglion. The pelvic ganglion was dissected out and attached to a petri dish and cultured in vitro. All three factors (BDNF, NT-3 and NGF) stimulated neurite outgrowth of both sympathetic and parasympathetic neurites although BDNF and NT-3 had a higher stimulatory effect on parasympathetic ganglion cells. The neurotrophin receptors TrkA, TrkB and TrkC were all expressed in neurons of the ganglia. Co-culture of ganglia with urinary bladder tissue, but not diaphragm tissue, increased the sprouting rate of neurites. Active forms of BDNF and NT-3 were detected in urinary bladder tissue using western blotting whereas tissue from the diaphragm expressed NGF. Neurite outgrowth from the pelvic ganglion was inhibited by a TrkB receptor antagonist. We therefore suggest that the urinary bladder releases trophic factors, including BDNF and NT-3, which regulate neurite outgrowth via activation of neuronal Trk-receptors. These findings could influence future strategies for developing pharmaceuticals to improve re-innervation due to bladder pathologies.</p>},
  author       = {Ekman, Mari and Zhu, Baoyi and Swärd, Karl and Uvelius, Bengt},
  issn         = {1566-0702},
  keyword      = {BNDF,NGF,NT-3,Parasympathetic,Sympathetic,Trk receptors},
  language     = {eng},
  month        = {03},
  pages        = {41--49},
  publisher    = {Elsevier},
  series       = {Autonomic Neuroscience: Basic and Clinical},
  title        = {Neurite outgrowth in cultured mouse pelvic ganglia - Effects of neurotrophins and bladder tissue},
  url          = {http://dx.doi.org/10.1016/j.autneu.2017.03.004},
  volume       = {205},
  year         = {2017},
}