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A Rat Model of Alzheimer’s Disease Based on Abeta42 and Pro-oxidative Substances Exhibits Cognitive Deficit and Alterations in Glutamatergic and Cholinergic Neurotransmitter Systems.

Petrasek, Tomas ; Skurlova, Martina ; Maleninska, Kristyna ; Vojtechova, Iveta ; Kristofikova, Zdena ; Matuskova, Hana LU ; Sirova, Jana ; Vales, Karel ; Ripova, Daniela and Stuchlik, Ales (2016) In Frontiers in Aging Neuroscience 2016. p.1-12
Abstract
Alzheimer’s disease (AD) is one of the most serious human, medical, and socioeconomic burdens. Here we tested the hypothesis that a rat model of AD (Samaritan; Taconic Pharmaceuticals, USA) based on the application of amyloid beta42 (Abeta42) and the pro-oxidative substances ferrous sulfate heptahydrate and L-buthionine-(S, R)-sulfoximine, will exhibit cognitive deficits and disruption of the glutamatergic and cholinergic systems in the brain. Behavioral methods included the Morris water maze (MWM; long-term memory version) and the active allothetic place avoidance (AAPA) task (acquisition and reversal), testing spatial memory and different aspects of hippocampal function. Neurochemical methods included testing of the NR1/NR2A/NR2B... (More)
Alzheimer’s disease (AD) is one of the most serious human, medical, and socioeconomic burdens. Here we tested the hypothesis that a rat model of AD (Samaritan; Taconic Pharmaceuticals, USA) based on the application of amyloid beta42 (Abeta42) and the pro-oxidative substances ferrous sulfate heptahydrate and L-buthionine-(S, R)-sulfoximine, will exhibit cognitive deficits and disruption of the glutamatergic and cholinergic systems in the brain. Behavioral methods included the Morris water maze (MWM; long-term memory version) and the active allothetic place avoidance (AAPA) task (acquisition and reversal), testing spatial memory and different aspects of hippocampal function. Neurochemical methods included testing of the NR1/NR2A/NR2B subunits of NMDA receptors in the frontal cortex and CHT1 transporters in the hippocampus, in both cases in the right and left hemisphere separately. Our results show that Samaritan rats™ exhibit marked impairment in both the MWM and active place avoidance tasks, suggesting a deficit of spatial learning and memory. Moreover, Samaritan rats exhibited significant changes in NR2A expression and CHT1 activity compared to controls rats, mimicking the situation in patients with early stage AD. Taken together, our results corroborate the hypothesis that Samaritan rats are a promising model of AD in its early stages. (Less)
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publishing date
type
Contribution to journal
publication status
published
subject
in
Frontiers in Aging Neuroscience
volume
2016
article number
00083
pages
1 - 12
publisher
Frontiers Media S. A.
external identifiers
  • scopus:84974533479
ISSN
1663-4365
DOI
10.3389/fnagi.2016.00083
language
English
LU publication?
no
id
ea1b0f0a-31e1-4664-b76d-78c556a8563c
date added to LUP
2020-02-01 10:31:11
date last changed
2022-02-16 02:27:09
@article{ea1b0f0a-31e1-4664-b76d-78c556a8563c,
  abstract     = {{Alzheimer’s disease (AD) is one of the most serious human, medical, and socioeconomic burdens. Here we tested the hypothesis that a rat model of AD (Samaritan; Taconic Pharmaceuticals, USA) based on the application of amyloid beta42 (Abeta42) and the pro-oxidative substances ferrous sulfate heptahydrate and L-buthionine-(S, R)-sulfoximine, will exhibit cognitive deficits and disruption of the glutamatergic and cholinergic systems in the brain. Behavioral methods included the Morris water maze (MWM; long-term memory version) and the active allothetic place avoidance (AAPA) task (acquisition and reversal), testing spatial memory and different aspects of hippocampal function. Neurochemical methods included testing of the NR1/NR2A/NR2B subunits of NMDA receptors in the frontal cortex and CHT1 transporters in the hippocampus, in both cases in the right and left hemisphere separately. Our results show that Samaritan rats™ exhibit marked impairment in both the MWM and active place avoidance tasks, suggesting a deficit of spatial learning and memory. Moreover, Samaritan rats exhibited significant changes in NR2A expression and CHT1 activity compared to controls rats, mimicking the situation in patients with early stage AD. Taken together, our results corroborate the hypothesis that Samaritan rats are a promising model of AD in its early stages.}},
  author       = {{Petrasek, Tomas and Skurlova, Martina and Maleninska, Kristyna and Vojtechova, Iveta and Kristofikova, Zdena and Matuskova, Hana and Sirova, Jana and Vales, Karel and Ripova, Daniela and Stuchlik, Ales}},
  issn         = {{1663-4365}},
  language     = {{eng}},
  pages        = {{1--12}},
  publisher    = {{Frontiers Media S. A.}},
  series       = {{Frontiers in Aging Neuroscience}},
  title        = {{A Rat Model of Alzheimer’s Disease Based on Abeta42 and Pro-oxidative Substances Exhibits Cognitive Deficit and Alterations in Glutamatergic and Cholinergic Neurotransmitter Systems.}},
  url          = {{http://dx.doi.org/10.3389/fnagi.2016.00083}},
  doi          = {{10.3389/fnagi.2016.00083}},
  volume       = {{2016}},
  year         = {{2016}},
}