Influence of strain and cultivation procedure on the performance of simultaneous saccharification and fermentation of steam pretreated spruce
(2006) In Enzyme and Microbial Technology 38(1-2). p.279-286- Abstract
- Yeast to be used in simultaneous saccharification and fermentation (SSF) of lignocelluloses materials has to be prepared in a separate cultivation step. The effects of the cultivation procedure on the performance of SSF of steam pretreated softwood were studied in the current work. The yeast used in the SSF was either directly commercially available Baker's yeast (as packaged yeast) or the same strain of yeast produced from the hydrolysate obtained in the pretreatment of the softwood material. A second strain of Saccharomyces cerevisiae TMB3000. isolated from spent sulphite liquor, was also compared with the commercial Baker's yeast. The strains were tested in SSF at substrate loads of 3, 5 and 8% dry weight of water insoluble material.... (More)
- Yeast to be used in simultaneous saccharification and fermentation (SSF) of lignocelluloses materials has to be prepared in a separate cultivation step. The effects of the cultivation procedure on the performance of SSF of steam pretreated softwood were studied in the current work. The yeast used in the SSF was either directly commercially available Baker's yeast (as packaged yeast) or the same strain of yeast produced from the hydrolysate obtained in the pretreatment of the softwood material. A second strain of Saccharomyces cerevisiae TMB3000. isolated from spent sulphite liquor, was also compared with the commercial Baker's yeast. The strains were tested in SSF at substrate loads of 3, 5 and 8% dry weight of water insoluble material. Final ethanol yields were above 85% of the theoretical (based on the available hexoses) in all cases, except for the package yeast for the 8% substrate load, in which case the final yield was less than 65%. The cultivation procedure was found to have a significant impact on the performance during SSF, as well as in small-scale fermentations of hydrolysate liquor without solid material. The Baker's yeast cultivated on the hydrolysate from the steam pretreatment had in all cases a higher productivity, in particular at the highest substrate load. Cultivated Baker's yeast had a slightly higher productivity than TMB3000. The results suggest that the adaptation of the yeast to the inhibitors present in the medium is an important factor that must be considered in the design of SSF processes. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/421689
- author
- Alkasrawi, Malek LU ; Rudolf, Andreas LU ; Lidén, Gunnar LU and Zacchi, Guido LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- ethanol, steam pretreated spruce, fermentation, simultaneous saccharification and, strain, cultivation procedure
- in
- Enzyme and Microbial Technology
- volume
- 38
- issue
- 1-2
- pages
- 279 - 286
- publisher
- Elsevier
- external identifiers
-
- wos:000234199300041
- scopus:27944495636
- ISSN
- 0141-0229
- DOI
- 10.1016/j.enzmictec.2005.08.024
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Chemical Engineering (011001014), Analytical Chemistry (S/LTH) (011001004)
- id
- eb5213ff-950d-4f77-be88-bcdce0bdc824 (old id 421689)
- date added to LUP
- 2016-04-01 11:59:21
- date last changed
- 2023-11-11 08:24:20
@article{eb5213ff-950d-4f77-be88-bcdce0bdc824,
abstract = {{Yeast to be used in simultaneous saccharification and fermentation (SSF) of lignocelluloses materials has to be prepared in a separate cultivation step. The effects of the cultivation procedure on the performance of SSF of steam pretreated softwood were studied in the current work. The yeast used in the SSF was either directly commercially available Baker's yeast (as packaged yeast) or the same strain of yeast produced from the hydrolysate obtained in the pretreatment of the softwood material. A second strain of Saccharomyces cerevisiae TMB3000. isolated from spent sulphite liquor, was also compared with the commercial Baker's yeast. The strains were tested in SSF at substrate loads of 3, 5 and 8% dry weight of water insoluble material. Final ethanol yields were above 85% of the theoretical (based on the available hexoses) in all cases, except for the package yeast for the 8% substrate load, in which case the final yield was less than 65%. The cultivation procedure was found to have a significant impact on the performance during SSF, as well as in small-scale fermentations of hydrolysate liquor without solid material. The Baker's yeast cultivated on the hydrolysate from the steam pretreatment had in all cases a higher productivity, in particular at the highest substrate load. Cultivated Baker's yeast had a slightly higher productivity than TMB3000. The results suggest that the adaptation of the yeast to the inhibitors present in the medium is an important factor that must be considered in the design of SSF processes.}},
author = {{Alkasrawi, Malek and Rudolf, Andreas and Lidén, Gunnar and Zacchi, Guido}},
issn = {{0141-0229}},
keywords = {{ethanol; steam pretreated spruce; fermentation; simultaneous saccharification and; strain; cultivation procedure}},
language = {{eng}},
number = {{1-2}},
pages = {{279--286}},
publisher = {{Elsevier}},
series = {{Enzyme and Microbial Technology}},
title = {{Influence of strain and cultivation procedure on the performance of simultaneous saccharification and fermentation of steam pretreated spruce}},
url = {{http://dx.doi.org/10.1016/j.enzmictec.2005.08.024}},
doi = {{10.1016/j.enzmictec.2005.08.024}},
volume = {{38}},
year = {{2006}},
}