Water activity control in enzymatic esterification processes
Wehtje, Ernst LU ; Kaur, Jasmedh ; Adlercreutz, Patrick LU
; Chand, Subhash
and Mattiasson, Bo
LU
(1997)
In Enzyme and Microbial Technology
21(7).
p.502-510
- Abstract
Lipase-catalyzed (Lipozyme IM 20 and Novozyme SP 435) esterification reactions in 5-methyl-2-hexanone were studied in order to investigate the practical application of a water activity control system consisting of a semipermeable silicone tubing and saturated salt solutions. The system used successfully in different reactor configurations [butch, continuous stirred-tank reactor (CSTR), and tubular] was described. The dimensions of the tubing could be calculated given the diffusion coefficient of water (D(aw)) through the tubing, the amount of enzyme, and the maximum accepted increase in water activity. Tubular reactors with continuous water activity control were constructed. The enzyme preparation was packed in a silicone tubing which... (More)
Lipase-catalyzed (Lipozyme IM 20 and Novozyme SP 435) esterification reactions in 5-methyl-2-hexanone were studied in order to investigate the practical application of a water activity control system consisting of a semipermeable silicone tubing and saturated salt solutions. The system used successfully in different reactor configurations [butch, continuous stirred-tank reactor (CSTR), and tubular] was described. The dimensions of the tubing could be calculated given the diffusion coefficient of water (D(aw)) through the tubing, the amount of enzyme, and the maximum accepted increase in water activity. Tubular reactors with continuous water activity control were constructed. The enzyme preparation was packed in a silicone tubing which was submerged into a saturated salt solution. A reactor Lipozyme was successfully used for two months without any decrease in product yield (72% conversion at a(w) = 0.33). By connecting a second tubular reactor (containing immobilized lipase and molecular sieves), the yield increased to 95%. A two-stage tubular reactor was used to catalyze esterification in pure substrates (equimolar amounts). The yield (after the second reactor) reached equilibrium (95% ester) at a water activity of 0.33.
(Less)
- author
- Wehtje, Ernst
LU
; Kaur, Jasmedh
; Adlercreutz, Patrick
LU
; Chand, Subhash
and Mattiasson, Bo
LU
- organization
- publishing date
- 1997-11-15
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Diffusion coefficient, Lipase, Molecular sieves, Neat substrate reactions, Reactor design, Silicone tubings
- in
- Enzyme and Microbial Technology
- volume
- 21
- issue
- 7
- pages
- 9 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0343907203
- ISSN
- 0141-0229
- DOI
- 10.1016/S0141-0229(97)00027-6
- language
- English
- LU publication?
- yes
- id
- eb865ec3-951e-410c-ac91-c4c794d4a32d
- date added to LUP
- 2019-06-20 16:12:49
- date last changed
- 2025-04-04 14:08:35
@article{eb865ec3-951e-410c-ac91-c4c794d4a32d,
abstract = {{<p>Lipase-catalyzed (Lipozyme IM 20 and Novozyme SP 435) esterification reactions in 5-methyl-2-hexanone were studied in order to investigate the practical application of a water activity control system consisting of a semipermeable silicone tubing and saturated salt solutions. The system used successfully in different reactor configurations [butch, continuous stirred-tank reactor (CSTR), and tubular] was described. The dimensions of the tubing could be calculated given the diffusion coefficient of water (D(aw)) through the tubing, the amount of enzyme, and the maximum accepted increase in water activity. Tubular reactors with continuous water activity control were constructed. The enzyme preparation was packed in a silicone tubing which was submerged into a saturated salt solution. A reactor Lipozyme was successfully used for two months without any decrease in product yield (72% conversion at a(w) = 0.33). By connecting a second tubular reactor (containing immobilized lipase and molecular sieves), the yield increased to 95%. A two-stage tubular reactor was used to catalyze esterification in pure substrates (equimolar amounts). The yield (after the second reactor) reached equilibrium (95% ester) at a water activity of 0.33.</p>}},
author = {{Wehtje, Ernst and Kaur, Jasmedh and Adlercreutz, Patrick and Chand, Subhash and Mattiasson, Bo}},
issn = {{0141-0229}},
keywords = {{Diffusion coefficient; Lipase; Molecular sieves; Neat substrate reactions; Reactor design; Silicone tubings}},
language = {{eng}},
month = {{11}},
number = {{7}},
pages = {{502--510}},
publisher = {{Elsevier}},
series = {{Enzyme and Microbial Technology}},
title = {{Water activity control in enzymatic esterification processes}},
url = {{http://dx.doi.org/10.1016/S0141-0229(97)00027-6}},
doi = {{10.1016/S0141-0229(97)00027-6}},
volume = {{21}},
year = {{1997}},
}