Focal traumatic brain injury induces neuroplastic molecular responses in lumbar spinal cord
(2019) In Restorative Neurology and Neuroscience 37(2). p.87-96- Abstract
Background/Objectives: Motor impairment induced by traumatic brain injury (TBI) may be mediated through changes in spinal molecular systems regulating neuronal plasticity. We assessed whether a focal controlled cortical impact (CCI) TBI in the rat alters expression of the Tgfb1, c-Fos, Bdnf, and Gap43 neuroplasticity genes in lumbar spinal cord. Approach/Methods: Adult male Sprague-Dawley rats (n = 8) were subjected to a right-side CCI over the anterior sensorimotor hindlimb representation area or sham-injury (n = 8). Absolute expression levels of Tgfb1, c-Fos, Bdnf, and Gapd43 genes were measured by droplet digital PCR in ipsi-and contralesional, dorsal and ventral quadrants of the L4 and L5 spinal cord. The neuronal activity marker... (More)
Background/Objectives: Motor impairment induced by traumatic brain injury (TBI) may be mediated through changes in spinal molecular systems regulating neuronal plasticity. We assessed whether a focal controlled cortical impact (CCI) TBI in the rat alters expression of the Tgfb1, c-Fos, Bdnf, and Gap43 neuroplasticity genes in lumbar spinal cord. Approach/Methods: Adult male Sprague-Dawley rats (n = 8) were subjected to a right-side CCI over the anterior sensorimotor hindlimb representation area or sham-injury (n = 8). Absolute expression levels of Tgfb1, c-Fos, Bdnf, and Gapd43 genes were measured by droplet digital PCR in ipsi-and contralesional, dorsal and ventral quadrants of the L4 and L5 spinal cord. The neuronal activity marker c-Fos was analysed by immunohistochemistry in the dorsal L4 and L5 segments. The contra- vs. ipsilesional expression pattern was examined as the asymmetry index, AI. Results: The Tgfb1 mRNA levels were significantly higher in the CCI vs. sham-injured rats, and in the contra- vs. ipsilesional dorsal domains in the CCI group. The number of c-Fos-positive cells was elevated in the L4 and L5 segments; and on the contralesional compared to the ipsilesional side in the CCI group. The c-Fos AI in the dorsal laminae was significantly increased by CCI. Conclusions: The results support the hypothesis that focal TBI induces plastic alterations in the lumbar spinal cord that may contribute to either motor recovery or maladaptive motor responses.
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- author
- Kononenko, Olga ; Watanabe, Hiroyuki LU ; Stålhandske, Lada ; Zarelius, Ann ; Clausen, Fredrik ; Yakovleva, Tatiana ; Bakalkin, Georgy and Marklund, Niklas LU
- organization
- publishing date
- 2019
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- c-Fos, plasticity, spinal cord, Tgfb1, Traumatic brain injury
- in
- Restorative Neurology and Neuroscience
- volume
- 37
- issue
- 2
- pages
- 10 pages
- publisher
- IOS Press
- external identifiers
-
- scopus:85064630764
- pmid:30856132
- ISSN
- 0922-6028
- DOI
- 10.3233/RNN-180882
- language
- English
- LU publication?
- yes
- id
- ec12badc-ea94-4ba9-b75e-68cc153cd8ec
- date added to LUP
- 2019-05-07 12:06:04
- date last changed
- 2024-07-09 11:44:57
@article{ec12badc-ea94-4ba9-b75e-68cc153cd8ec, abstract = {{<p>Background/Objectives: Motor impairment induced by traumatic brain injury (TBI) may be mediated through changes in spinal molecular systems regulating neuronal plasticity. We assessed whether a focal controlled cortical impact (CCI) TBI in the rat alters expression of the Tgfb1, c-Fos, Bdnf, and Gap43 neuroplasticity genes in lumbar spinal cord. Approach/Methods: Adult male Sprague-Dawley rats (n = 8) were subjected to a right-side CCI over the anterior sensorimotor hindlimb representation area or sham-injury (n = 8). Absolute expression levels of Tgfb1, c-Fos, Bdnf, and Gapd43 genes were measured by droplet digital PCR in ipsi-and contralesional, dorsal and ventral quadrants of the L4 and L5 spinal cord. The neuronal activity marker c-Fos was analysed by immunohistochemistry in the dorsal L4 and L5 segments. The contra- vs. ipsilesional expression pattern was examined as the asymmetry index, AI. Results: The Tgfb1 mRNA levels were significantly higher in the CCI vs. sham-injured rats, and in the contra- vs. ipsilesional dorsal domains in the CCI group. The number of c-Fos-positive cells was elevated in the L4 and L5 segments; and on the contralesional compared to the ipsilesional side in the CCI group. The c-Fos AI in the dorsal laminae was significantly increased by CCI. Conclusions: The results support the hypothesis that focal TBI induces plastic alterations in the lumbar spinal cord that may contribute to either motor recovery or maladaptive motor responses.</p>}}, author = {{Kononenko, Olga and Watanabe, Hiroyuki and Stålhandske, Lada and Zarelius, Ann and Clausen, Fredrik and Yakovleva, Tatiana and Bakalkin, Georgy and Marklund, Niklas}}, issn = {{0922-6028}}, keywords = {{c-Fos; plasticity; spinal cord; Tgfb1; Traumatic brain injury}}, language = {{eng}}, number = {{2}}, pages = {{87--96}}, publisher = {{IOS Press}}, series = {{Restorative Neurology and Neuroscience}}, title = {{Focal traumatic brain injury induces neuroplastic molecular responses in lumbar spinal cord}}, url = {{http://dx.doi.org/10.3233/RNN-180882}}, doi = {{10.3233/RNN-180882}}, volume = {{37}}, year = {{2019}}, }