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Focal traumatic brain injury induces neuroplastic molecular responses in lumbar spinal cord

Kononenko, Olga; Watanabe, Hiroyuki LU ; Stålhandske, Lada; Zarelius, Ann; Clausen, Fredrik; Yakovleva, Tatiana; Bakalkin, Georgy and Marklund, Niklas LU (2019) In Restorative Neurology and Neuroscience 37(2). p.87-96
Abstract

Background/Objectives: Motor impairment induced by traumatic brain injury (TBI) may be mediated through changes in spinal molecular systems regulating neuronal plasticity. We assessed whether a focal controlled cortical impact (CCI) TBI in the rat alters expression of the Tgfb1, c-Fos, Bdnf, and Gap43 neuroplasticity genes in lumbar spinal cord. Approach/Methods: Adult male Sprague-Dawley rats (n = 8) were subjected to a right-side CCI over the anterior sensorimotor hindlimb representation area or sham-injury (n = 8). Absolute expression levels of Tgfb1, c-Fos, Bdnf, and Gapd43 genes were measured by droplet digital PCR in ipsi-and contralesional, dorsal and ventral quadrants of the L4 and L5 spinal cord. The neuronal activity marker... (More)

Background/Objectives: Motor impairment induced by traumatic brain injury (TBI) may be mediated through changes in spinal molecular systems regulating neuronal plasticity. We assessed whether a focal controlled cortical impact (CCI) TBI in the rat alters expression of the Tgfb1, c-Fos, Bdnf, and Gap43 neuroplasticity genes in lumbar spinal cord. Approach/Methods: Adult male Sprague-Dawley rats (n = 8) were subjected to a right-side CCI over the anterior sensorimotor hindlimb representation area or sham-injury (n = 8). Absolute expression levels of Tgfb1, c-Fos, Bdnf, and Gapd43 genes were measured by droplet digital PCR in ipsi-and contralesional, dorsal and ventral quadrants of the L4 and L5 spinal cord. The neuronal activity marker c-Fos was analysed by immunohistochemistry in the dorsal L4 and L5 segments. The contra- vs. ipsilesional expression pattern was examined as the asymmetry index, AI. Results: The Tgfb1 mRNA levels were significantly higher in the CCI vs. sham-injured rats, and in the contra- vs. ipsilesional dorsal domains in the CCI group. The number of c-Fos-positive cells was elevated in the L4 and L5 segments; and on the contralesional compared to the ipsilesional side in the CCI group. The c-Fos AI in the dorsal laminae was significantly increased by CCI. Conclusions: The results support the hypothesis that focal TBI induces plastic alterations in the lumbar spinal cord that may contribute to either motor recovery or maladaptive motor responses.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
c-Fos, plasticity, spinal cord, Tgfb1, Traumatic brain injury
in
Restorative Neurology and Neuroscience
volume
37
issue
2
pages
10 pages
publisher
IOS Press
external identifiers
  • scopus:85064630764
ISSN
0922-6028
DOI
10.3233/RNN-180882
language
English
LU publication?
yes
id
ec12badc-ea94-4ba9-b75e-68cc153cd8ec
date added to LUP
2019-05-07 12:06:04
date last changed
2019-10-06 04:39:18
@article{ec12badc-ea94-4ba9-b75e-68cc153cd8ec,
  abstract     = {<p>Background/Objectives: Motor impairment induced by traumatic brain injury (TBI) may be mediated through changes in spinal molecular systems regulating neuronal plasticity. We assessed whether a focal controlled cortical impact (CCI) TBI in the rat alters expression of the Tgfb1, c-Fos, Bdnf, and Gap43 neuroplasticity genes in lumbar spinal cord. Approach/Methods: Adult male Sprague-Dawley rats (n = 8) were subjected to a right-side CCI over the anterior sensorimotor hindlimb representation area or sham-injury (n = 8). Absolute expression levels of Tgfb1, c-Fos, Bdnf, and Gapd43 genes were measured by droplet digital PCR in ipsi-and contralesional, dorsal and ventral quadrants of the L4 and L5 spinal cord. The neuronal activity marker c-Fos was analysed by immunohistochemistry in the dorsal L4 and L5 segments. The contra- vs. ipsilesional expression pattern was examined as the asymmetry index, AI. Results: The Tgfb1 mRNA levels were significantly higher in the CCI vs. sham-injured rats, and in the contra- vs. ipsilesional dorsal domains in the CCI group. The number of c-Fos-positive cells was elevated in the L4 and L5 segments; and on the contralesional compared to the ipsilesional side in the CCI group. The c-Fos AI in the dorsal laminae was significantly increased by CCI. Conclusions: The results support the hypothesis that focal TBI induces plastic alterations in the lumbar spinal cord that may contribute to either motor recovery or maladaptive motor responses.</p>},
  author       = {Kononenko, Olga and Watanabe, Hiroyuki and Stålhandske, Lada and Zarelius, Ann and Clausen, Fredrik and Yakovleva, Tatiana and Bakalkin, Georgy and Marklund, Niklas},
  issn         = {0922-6028},
  keyword      = {c-Fos,plasticity,spinal cord,Tgfb1,Traumatic brain injury},
  language     = {eng},
  number       = {2},
  pages        = {87--96},
  publisher    = {IOS Press},
  series       = {Restorative Neurology and Neuroscience},
  title        = {Focal traumatic brain injury induces neuroplastic molecular responses in lumbar spinal cord},
  url          = {http://dx.doi.org/10.3233/RNN-180882},
  volume       = {37},
  year         = {2019},
}