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Structural model of human alpha1-microglobulin : proposed scheme for the interaction with the Gla domain of anticoagulant protein C

Villoutreix, B O ; Akerström, B LU and Lindqvist, A LU (2000) In Blood Coagulation and Fibrinolysis 11(3). p.75-261
Abstract

Alpha1-microglobulin (alpha1m) is a small glycoprotein with immunomodulatory properties. It is a member of the lipocalin family, a group of proteins that exhibit a well-conserved three-dimensional structure despite low sequence identity and that are known to bind small hydrophobic ligands. The types of ligands carried by alpha1m are still unknown, but it is known that this protein has yellow-brown chromophores attached to three lysines at position 92, 118 and 130. Alpha1m has one unpaired cysteine residue (Cys 34) that can form a disulphide bond with other proteins that also possess an exposed free unpaired cysteine. For instance, alpha1m interacts with the protein C (PC) Gla domain containing the Arg9Cys or Ser12Cys substitution. In... (More)

Alpha1-microglobulin (alpha1m) is a small glycoprotein with immunomodulatory properties. It is a member of the lipocalin family, a group of proteins that exhibit a well-conserved three-dimensional structure despite low sequence identity and that are known to bind small hydrophobic ligands. The types of ligands carried by alpha1m are still unknown, but it is known that this protein has yellow-brown chromophores attached to three lysines at position 92, 118 and 130. Alpha1m has one unpaired cysteine residue (Cys 34) that can form a disulphide bond with other proteins that also possess an exposed free unpaired cysteine. For instance, alpha1m interacts with the protein C (PC) Gla domain containing the Arg9Cys or Ser12Cys substitution. In order to gain insights about the alpha1m molecule and analyze the intriguing alpha1m-Gla domain interaction, it was decided to use bioinformatics. The three-dimensional structures of alpha1m and PC Gla domain were predicted. Alpha1m Cys 34 is solvent exposed and located near the entrance of the ligand-binding pocket. The chromophore-carrying lysines are found buried into the pocket, and the area around the entrance of this cavity displays about 10 positively charged residues. This electropositive region in alpha1m complements the essentially electronegative Gla domain and may play a role during intermolecular interactions. In addition, a few hydrophobic residues surround alpha1m Cys 34 and could be of importance during its interaction with macromolecular ligands.

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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Amino Acid Sequence, Humans, Membrane Glycoproteins/chemistry, Models, Molecular, Molecular Sequence Data, Protein Binding, Protein C/chemistry, Protein Conformation, Structure-Activity Relationship, Trypsin Inhibitor, Kunitz Soybean
in
Blood Coagulation and Fibrinolysis
volume
11
issue
3
pages
75 - 261
publisher
Lippincott Williams & Wilkins
external identifiers
  • pmid:10870807
ISSN
0957-5235
language
English
LU publication?
yes
id
ecf89b63-1f34-4325-8752-de6c64288d5e
date added to LUP
2019-05-22 10:08:30
date last changed
2020-11-19 04:02:12
@article{ecf89b63-1f34-4325-8752-de6c64288d5e,
  abstract     = {<p>Alpha1-microglobulin (alpha1m) is a small glycoprotein with immunomodulatory properties. It is a member of the lipocalin family, a group of proteins that exhibit a well-conserved three-dimensional structure despite low sequence identity and that are known to bind small hydrophobic ligands. The types of ligands carried by alpha1m are still unknown, but it is known that this protein has yellow-brown chromophores attached to three lysines at position 92, 118 and 130. Alpha1m has one unpaired cysteine residue (Cys 34) that can form a disulphide bond with other proteins that also possess an exposed free unpaired cysteine. For instance, alpha1m interacts with the protein C (PC) Gla domain containing the Arg9Cys or Ser12Cys substitution. In order to gain insights about the alpha1m molecule and analyze the intriguing alpha1m-Gla domain interaction, it was decided to use bioinformatics. The three-dimensional structures of alpha1m and PC Gla domain were predicted. Alpha1m Cys 34 is solvent exposed and located near the entrance of the ligand-binding pocket. The chromophore-carrying lysines are found buried into the pocket, and the area around the entrance of this cavity displays about 10 positively charged residues. This electropositive region in alpha1m complements the essentially electronegative Gla domain and may play a role during intermolecular interactions. In addition, a few hydrophobic residues surround alpha1m Cys 34 and could be of importance during its interaction with macromolecular ligands.</p>},
  author       = {Villoutreix, B O and Akerström, B and Lindqvist, A},
  issn         = {0957-5235},
  language     = {eng},
  number       = {3},
  pages        = {75--261},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {Blood Coagulation and Fibrinolysis},
  title        = {Structural model of human alpha1-microglobulin : proposed scheme for the interaction with the Gla domain of anticoagulant protein C},
  volume       = {11},
  year         = {2000},
}