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Cytokines induce an epithelial cell cytokine response

Hedges, S.; Svensson, M. LU ; Agace, W. LU and Svanborg, C. LU (1995) In Advances in Experimental Medicine and Biology 371(A). p.189-193
Abstract
Intravesical inoculation of gram negative bacteria or isolated bacterial products into mice induces an IL-6 response which can be measured within miutes of the infection.1,2 In such infections, IL-6 is initially detected in the urine and subsequently in the serum. Similar IL-6 responses were found in human patients deliberately colonized in the urinary tract with E. coli Hu7343. IL-6 was secreted intermittantly into the urine in response to continuous bacterial infection in humans, but was not detected in serum. The rapid secretion of IL-6 into urine after bacterial stimulation, and the separation of local from systemic secretion in both humans and mice suggested that IL-6 was produced at the site of infection. Since epithelial cells... (More)
Intravesical inoculation of gram negative bacteria or isolated bacterial products into mice induces an IL-6 response which can be measured within miutes of the infection.1,2 In such infections, IL-6 is initially detected in the urine and subsequently in the serum. Similar IL-6 responses were found in human patients deliberately colonized in the urinary tract with E. coli Hu7343. IL-6 was secreted intermittantly into the urine in response to continuous bacterial infection in humans, but was not detected in serum. The rapid secretion of IL-6 into urine after bacterial stimulation, and the separation of local from systemic secretion in both humans and mice suggested that IL-6 was produced at the site of infection. Since epithelial cells dominate the naive urinary tract mucosal surface, we suggested that epithelial cells are one source of mucosally produced cytokines.4 Epithelial cell lines of urinary tract origin secrete IL-6 and IL-8, and can produce other cytokines after bacterial stimulation.5–7 In this paper we demonstrate that epithelial cell lines of urinary tract origin can respond to exogenous cytokines. This response includes the up-regulation of a variety of cytokine mRNA species as well as secretion of IL-6 and IL-8. Furthermore, this response is specific to the stimulus used, with different cytokine mRNA species induced by different cytokine stimuli. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Advances in Experimental Medicine and Biology
volume
371
issue
A
pages
5 pages
publisher
Kluwer
external identifiers
  • scopus:0029120247
ISSN
0065-2598
DOI
10.1007/978-1-4615-1941-6_38
language
English
LU publication?
yes
id
ed4ccecc-567e-4df4-8a04-9bd49b19a539
date added to LUP
2019-05-30 13:47:37
date last changed
2019-08-16 14:28:52
@article{ed4ccecc-567e-4df4-8a04-9bd49b19a539,
  abstract     = {Intravesical inoculation of gram negative bacteria or isolated bacterial products into mice induces an IL-6 response which can be measured within miutes of the infection.1,2 In such infections, IL-6 is initially detected in the urine and subsequently in the serum. Similar IL-6 responses were found in human patients deliberately colonized in the urinary tract with E. coli Hu7343. IL-6 was secreted intermittantly into the urine in response to continuous bacterial infection in humans, but was not detected in serum. The rapid secretion of IL-6 into urine after bacterial stimulation, and the separation of local from systemic secretion in both humans and mice suggested that IL-6 was produced at the site of infection. Since epithelial cells dominate the naive urinary tract mucosal surface, we suggested that epithelial cells are one source of mucosally produced cytokines.4 Epithelial cell lines of urinary tract origin secrete IL-6 and IL-8, and can produce other cytokines after bacterial stimulation.5–7 In this paper we demonstrate that epithelial cell lines of urinary tract origin can respond to exogenous cytokines. This response includes the up-regulation of a variety of cytokine mRNA species as well as secretion of IL-6 and IL-8. Furthermore, this response is specific to the stimulus used, with different cytokine mRNA species induced by different cytokine stimuli.},
  author       = {Hedges, S. and Svensson, M. and Agace, W. and Svanborg, C.},
  issn         = {0065-2598},
  language     = {eng},
  month        = {01},
  number       = {A},
  pages        = {189--193},
  publisher    = {Kluwer},
  series       = {Advances in Experimental Medicine and Biology},
  title        = {Cytokines induce an epithelial cell cytokine response},
  url          = {http://dx.doi.org/10.1007/978-1-4615-1941-6_38},
  volume       = {371},
  year         = {1995},
}