Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Determination of the self-diffusion coefficient of intracellular water using PGSE NMR with variable gradient pulse length.

Åslund, Ingrid LU and Topgaard, Daniel LU (2009) In Journal of Magnetic Resonance 201(2). p.250-254
Abstract
A new pulsed-gradient spin-echo NMR protocol for assessing the local self-diffusion coefficient D(0) of water confined within living cells is proposed. Equations for the apparent mean-square displacement <Z(2)> as a function of the effective diffusion time t(d) and the duration of the displacement-encoding gradient pulses delta are derived. The standard method of estimating D(0) - reducing t(d) until the influence of collisions between the water molecules and the plasma membrane can be neglected - often fails because of the small size of typical cells. As demonstrated here, the decrease of the apparent <Z(2)> with increasing delta at constant t(d) can be utilized to measure D(0).
Please use this url to cite or link to this publication:
author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Magnetic Resonance
volume
201
issue
2
pages
250 - 254
publisher
Academic Press
external identifiers
  • wos:000272006600017
  • pmid:19800273
  • scopus:70350617673
  • pmid:19800273
ISSN
1096-0856
DOI
10.1016/j.jmr.2009.09.006
language
English
LU publication?
yes
id
ef7e32ba-1b6b-4f38-84cc-d17419856a70 (old id 1500734)
date added to LUP
2016-04-01 13:15:54
date last changed
2022-03-29 06:29:56
@article{ef7e32ba-1b6b-4f38-84cc-d17419856a70,
  abstract     = {{A new pulsed-gradient spin-echo NMR protocol for assessing the local self-diffusion coefficient D(0) of water confined within living cells is proposed. Equations for the apparent mean-square displacement &lt;Z(2)&gt; as a function of the effective diffusion time t(d) and the duration of the displacement-encoding gradient pulses delta are derived. The standard method of estimating D(0) - reducing t(d) until the influence of collisions between the water molecules and the plasma membrane can be neglected - often fails because of the small size of typical cells. As demonstrated here, the decrease of the apparent &lt;Z(2)&gt; with increasing delta at constant t(d) can be utilized to measure D(0).}},
  author       = {{Åslund, Ingrid and Topgaard, Daniel}},
  issn         = {{1096-0856}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{250--254}},
  publisher    = {{Academic Press}},
  series       = {{Journal of Magnetic Resonance}},
  title        = {{Determination of the self-diffusion coefficient of intracellular water using PGSE NMR with variable gradient pulse length.}},
  url          = {{http://dx.doi.org/10.1016/j.jmr.2009.09.006}},
  doi          = {{10.1016/j.jmr.2009.09.006}},
  volume       = {{201}},
  year         = {{2009}},
}