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Proteomic differences between focal and diffuse traumatic brain injury in human brain tissue

Abu Hamdeh, Sami ; Shevchenko, Ganna ; Mi, Jia ; Musunuri, Sravani ; Bergquist, Jonas and Marklund, Niklas LU orcid (2018) In Scientific Reports 8(1). p.1-15
Abstract

The early molecular response to severe traumatic brain injury (TBI) was evaluated using biopsies of structurally normal-appearing cortex, obtained at location for intracranial pressure (ICP) monitoring, from 16 severe TBI patients. Mass spectrometry (MS; label free and stable isotope dimethyl labeling) quantitation proteomics showed a strikingly different molecular pattern in TBI in comparison to cortical biopsies from 11 idiopathic normal pressure hydrocephalus patients. Diffuse TBI showed increased expression of peptides related to neurodegeneration (Tau and Fascin, p < 0.05), reduced expression related to antioxidant defense (Glutathione S-transferase Mu 3, Peroxiredoxin-6, Thioredoxin-dependent peroxide reductase; p < 0.05)... (More)

The early molecular response to severe traumatic brain injury (TBI) was evaluated using biopsies of structurally normal-appearing cortex, obtained at location for intracranial pressure (ICP) monitoring, from 16 severe TBI patients. Mass spectrometry (MS; label free and stable isotope dimethyl labeling) quantitation proteomics showed a strikingly different molecular pattern in TBI in comparison to cortical biopsies from 11 idiopathic normal pressure hydrocephalus patients. Diffuse TBI showed increased expression of peptides related to neurodegeneration (Tau and Fascin, p < 0.05), reduced expression related to antioxidant defense (Glutathione S-transferase Mu 3, Peroxiredoxin-6, Thioredoxin-dependent peroxide reductase; p < 0.05) and increased expression of potential biomarkers (e.g. Neurogranin, Fatty acid-binding protein, heart p < 0.05) compared to focal TBI. Proteomics of human brain biopsies displayed considerable molecular heterogeneity among the different TBI subtypes with consequences for the pathophysiology and development of targeted treatments for TBI.

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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Scientific Reports
volume
8
issue
1
article number
6807
pages
1 - 15
publisher
Nature Publishing Group
external identifiers
  • pmid:29717219
  • scopus:85046409895
ISSN
2045-2322
DOI
10.1038/s41598-018-25060-0
language
English
LU publication?
yes
id
ef854d11-c40d-4aa7-8fd4-a515c2ce2084
date added to LUP
2018-09-13 15:19:02
date last changed
2025-04-15 11:34:57
@article{ef854d11-c40d-4aa7-8fd4-a515c2ce2084,
  abstract     = {{<p>The early molecular response to severe traumatic brain injury (TBI) was evaluated using biopsies of structurally normal-appearing cortex, obtained at location for intracranial pressure (ICP) monitoring, from 16 severe TBI patients. Mass spectrometry (MS; label free and stable isotope dimethyl labeling) quantitation proteomics showed a strikingly different molecular pattern in TBI in comparison to cortical biopsies from 11 idiopathic normal pressure hydrocephalus patients. Diffuse TBI showed increased expression of peptides related to neurodegeneration (Tau and Fascin, p &lt; 0.05), reduced expression related to antioxidant defense (Glutathione S-transferase Mu 3, Peroxiredoxin-6, Thioredoxin-dependent peroxide reductase; p &lt; 0.05) and increased expression of potential biomarkers (e.g. Neurogranin, Fatty acid-binding protein, heart p &lt; 0.05) compared to focal TBI. Proteomics of human brain biopsies displayed considerable molecular heterogeneity among the different TBI subtypes with consequences for the pathophysiology and development of targeted treatments for TBI.</p>}},
  author       = {{Abu Hamdeh, Sami and Shevchenko, Ganna and Mi, Jia and Musunuri, Sravani and Bergquist, Jonas and Marklund, Niklas}},
  issn         = {{2045-2322}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{1}},
  pages        = {{1--15}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Scientific Reports}},
  title        = {{Proteomic differences between focal and diffuse traumatic brain injury in human brain tissue}},
  url          = {{http://dx.doi.org/10.1038/s41598-018-25060-0}},
  doi          = {{10.1038/s41598-018-25060-0}},
  volume       = {{8}},
  year         = {{2018}},
}