Physical characterization of porous materials and correlation with the activity of immobilized enzyme in organic medium
Barros, Raúl J. LU ; Wehtje, Ernst LU ; Garcia, Fernando A.P. and Adlercreutz, Patrick LU
(1998)
In Biocatalysis and Biotransformation
16(1).
p.67-85
- Abstract
A series of commonly used porous supports was characterized by determination of particle size distribution, porosity, surface area (total and distributions with pore diameters) and skeletal density. The performance of immobilized α-chymotrypsin catalyzed dipeptide synthesis in an acetonitrile medium was correlated with these physical properties.At high enzyme loading, when internal mass transfer limitations are expected to occur, the activity can be correlated with the support characteristic parameter. This is a combination of physical properties such as particle size, porosity, and volumetric porosity, which influence the substrate diffusion rate. At low enzyme loading the important parameter is the accessible surface area, which will... (More)
A series of commonly used porous supports was characterized by determination of particle size distribution, porosity, surface area (total and distributions with pore diameters) and skeletal density. The performance of immobilized α-chymotrypsin catalyzed dipeptide synthesis in an acetonitrile medium was correlated with these physical properties.At high enzyme loading, when internal mass transfer limitations are expected to occur, the activity can be correlated with the support characteristic parameter. This is a combination of physical properties such as particle size, porosity, and volumetric porosity, which influence the substrate diffusion rate. At low enzyme loading the important parameter is the accessible surface area, which will determine how the enzyme is distributed in the pores of the support. When assessing the effect of the support material on enzymatic activity, the geometric considerations studied here should always be contemplated before making any assumptions about direct effects of support material on enzymatic catalytic properties.
(Less)
- author
- Barros, Raúl J.
LU
; Wehtje, Ernst
LU
; Garcia, Fernando A.P.
and Adlercreutz, Patrick
LU
- organization
- publishing date
- 1998-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Immobilized α-chymotrypsin, Internal diffusion, Organic medium, Porous supports, Thiele modulus
- in
- Biocatalysis and Biotransformation
- volume
- 16
- issue
- 1
- pages
- 19 pages
- publisher
- Informa Healthcare
- external identifiers
-
- scopus:0031958027
- ISSN
- 1024-2422
- DOI
- 10.3109/10242429809040111
- language
- English
- LU publication?
- yes
- id
- ef9994dd-8aba-4084-ab72-a651efc7d1f3
- date added to LUP
- 2019-06-20 16:12:23
- date last changed
- 2025-04-04 14:39:33
@article{ef9994dd-8aba-4084-ab72-a651efc7d1f3,
abstract = {{<p>A series of commonly used porous supports was characterized by determination of particle size distribution, porosity, surface area (total and distributions with pore diameters) and skeletal density. The performance of immobilized α-chymotrypsin catalyzed dipeptide synthesis in an acetonitrile medium was correlated with these physical properties.At high enzyme loading, when internal mass transfer limitations are expected to occur, the activity can be correlated with the support characteristic parameter. This is a combination of physical properties such as particle size, porosity, and volumetric porosity, which influence the substrate diffusion rate. At low enzyme loading the important parameter is the accessible surface area, which will determine how the enzyme is distributed in the pores of the support. When assessing the effect of the support material on enzymatic activity, the geometric considerations studied here should always be contemplated before making any assumptions about direct effects of support material on enzymatic catalytic properties.</p>}},
author = {{Barros, Raúl J. and Wehtje, Ernst and Garcia, Fernando A.P. and Adlercreutz, Patrick}},
issn = {{1024-2422}},
keywords = {{Immobilized α-chymotrypsin; Internal diffusion; Organic medium; Porous supports; Thiele modulus}},
language = {{eng}},
month = {{01}},
number = {{1}},
pages = {{67--85}},
publisher = {{Informa Healthcare}},
series = {{Biocatalysis and Biotransformation}},
title = {{Physical characterization of porous materials and correlation with the activity of immobilized enzyme in organic medium}},
url = {{http://dx.doi.org/10.3109/10242429809040111}},
doi = {{10.3109/10242429809040111}},
volume = {{16}},
year = {{1998}},
}