Multiplex detection of human herpesviruses from archival specimens by using matrix-assisted laser desorption ionization-time of flight mass spectrometry.
(2008) In Journal of Clinical Microbiology 46(2). p.540-545- Abstract
- The human herpesviruses are involved in a variety of diseases. Large-scale evaluation of the clinical and epidemiological importance of different herpesviruses requires high-throughput methods. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a method that has a multiplex capacity enabling simultaneous detection of several viruses in a single sample. PCR-based methods for the multiplex detection of all known human herpesviruses were developed on the MALDI-TOF MS system. A variety of 882 archival samples, including bronchoalveolar lavage, conjunctival fluid, sore secretion, blister material, plasma, serum, and urine, analyzed for herpesviruses using PCR-based reference methods, were used to... (More)
- The human herpesviruses are involved in a variety of diseases. Large-scale evaluation of the clinical and epidemiological importance of different herpesviruses requires high-throughput methods. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a method that has a multiplex capacity enabling simultaneous detection of several viruses in a single sample. PCR-based methods for the multiplex detection of all known human herpesviruses were developed on the MALDI-TOF MS system. A variety of 882 archival samples, including bronchoalveolar lavage, conjunctival fluid, sore secretion, blister material, plasma, serum, and urine, analyzed for herpesviruses using PCR-based reference methods, were used to evaluate the MALDI-TOF MS method. The overall concordance rate between the MALDI-TOF MS method and the reference methods was 95.6% (kappa = 0.90). In summary, the MALDI-TOF MS method is well suited for large-scale detection of all known human herpesviruses in a wide variety of archival biological specimens. (Less)
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/record/1035062
- author
- Sjöholm, Malin LU ; Dillner, Joakim LU and Carlson, Joyce LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Clinical Microbiology
- volume
- 46
- issue
- 2
- pages
- 540 - 545
- publisher
- American Society for Microbiology
- external identifiers
-
- pmid:18094141
- wos:000253100300022
- scopus:38949194239
- ISSN
- 1098-660X
- DOI
- 10.1128/JCM.01565-07
- language
- English
- LU publication?
- yes
- id
- ef9cd724-9576-400c-9427-a56fae123bba (old id 1035062)
- date added to LUP
- 2008-07-31 12:36:08
- date last changed
- 2018-11-21 20:21:25
@article{ef9cd724-9576-400c-9427-a56fae123bba,
abstract = {The human herpesviruses are involved in a variety of diseases. Large-scale evaluation of the clinical and epidemiological importance of different herpesviruses requires high-throughput methods. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a method that has a multiplex capacity enabling simultaneous detection of several viruses in a single sample. PCR-based methods for the multiplex detection of all known human herpesviruses were developed on the MALDI-TOF MS system. A variety of 882 archival samples, including bronchoalveolar lavage, conjunctival fluid, sore secretion, blister material, plasma, serum, and urine, analyzed for herpesviruses using PCR-based reference methods, were used to evaluate the MALDI-TOF MS method. The overall concordance rate between the MALDI-TOF MS method and the reference methods was 95.6% (kappa = 0.90). In summary, the MALDI-TOF MS method is well suited for large-scale detection of all known human herpesviruses in a wide variety of archival biological specimens.},
author = {Sjöholm, Malin and Dillner, Joakim and Carlson, Joyce},
issn = {1098-660X},
language = {eng},
number = {2},
pages = {540--545},
publisher = {American Society for Microbiology},
series = {Journal of Clinical Microbiology},
title = {Multiplex detection of human herpesviruses from archival specimens by using matrix-assisted laser desorption ionization-time of flight mass spectrometry.},
url = {http://dx.doi.org/10.1128/JCM.01565-07},
volume = {46},
year = {2008},
}