Genetic dissection of lymphopenia from autoimmunity by introgression of mutated Ian5 gene onto the F344 rat.
(2003) In Journal of Autoimmunity 21(4). p.315-324- Abstract
- Peripheral T cell lymphopenia (lyp) in the BioBreeding (BB) rat is linked to a frameshift mutation in Ian5, a member of the Immune Associated Nucleotide (Ian) gene family on rat chromosome 4. This lymphopenia leads to type 1 (insulin-dependent) diabetes mellitus (T1DM) at rates up to 100% when combined with the BB rat MHC RT1 u/u genotype. In order, to better study the lymphopenia phenotype without possible confounding effects of diabetes or other autoimmune disease, we generated congenic F344.lyp rats by introgression of lyp on diabetes-resistant MHC RT1 lv1/lv1 F344 rats. Analysis of thymic CD4 and CD8 T lymphocytes revealed no difference in the percentage of CD4(-)CD8(+)and CD4(+)CD8(-)subsets in lyp/lyp compared to +/+ F344 rats. The... (More)
- Peripheral T cell lymphopenia (lyp) in the BioBreeding (BB) rat is linked to a frameshift mutation in Ian5, a member of the Immune Associated Nucleotide (Ian) gene family on rat chromosome 4. This lymphopenia leads to type 1 (insulin-dependent) diabetes mellitus (T1DM) at rates up to 100% when combined with the BB rat MHC RT1 u/u genotype. In order, to better study the lymphopenia phenotype without possible confounding effects of diabetes or other autoimmune disease, we generated congenic F344.lyp rats by introgression of lyp on diabetes-resistant MHC RT1 lv1/lv1 F344 rats. Analysis of thymic CD4 and CD8 T lymphocytes revealed no difference in the percentage of CD4(-)CD8(+)and CD4(+)CD8(-)subsets in lyp/lyp compared to +/+ F344 rats. The same subsets was however dramatically reduced in blood (P=0.005), spleen (P=0.019) and mesenteric lymph nodes (MLN) (P<0.0001). Compared to F344 +/+ rats double positive CD4(+)CD8(+)T cells were increased only in lyp/lyp spleen (P=0.034) while double n (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1127389
- author
- Moralejo, Daniel H ; Park, Hyunhee A ; Speros, Sara J ; MacMurray, Armand J ; Kwitek, Anne E ; Jacob, Howard J ; Lander, Eric S and Lernmark, Åke LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Autoimmunity
- volume
- 21
- issue
- 4
- pages
- 315 - 324
- publisher
- Elsevier
- external identifiers
-
- scopus:0242659141
- ISSN
- 0896-8411
- DOI
- 10.1016/S0896-8411(03)00138-0
- language
- English
- LU publication?
- yes
- id
- efa2e295-df21-4565-96e0-d7c2876aed76 (old id 1127389)
- date added to LUP
- 2016-04-01 11:41:31
- date last changed
- 2022-03-05 05:03:01
@article{efa2e295-df21-4565-96e0-d7c2876aed76, abstract = {{Peripheral T cell lymphopenia (lyp) in the BioBreeding (BB) rat is linked to a frameshift mutation in Ian5, a member of the Immune Associated Nucleotide (Ian) gene family on rat chromosome 4. This lymphopenia leads to type 1 (insulin-dependent) diabetes mellitus (T1DM) at rates up to 100% when combined with the BB rat MHC RT1 u/u genotype. In order, to better study the lymphopenia phenotype without possible confounding effects of diabetes or other autoimmune disease, we generated congenic F344.lyp rats by introgression of lyp on diabetes-resistant MHC RT1 lv1/lv1 F344 rats. Analysis of thymic CD4 and CD8 T lymphocytes revealed no difference in the percentage of CD4(-)CD8(+)and CD4(+)CD8(-)subsets in lyp/lyp compared to +/+ F344 rats. The same subsets was however dramatically reduced in blood (P=0.005), spleen (P=0.019) and mesenteric lymph nodes (MLN) (P<0.0001). Compared to F344 +/+ rats double positive CD4(+)CD8(+)T cells were increased only in lyp/lyp spleen (P=0.034) while double n}}, author = {{Moralejo, Daniel H and Park, Hyunhee A and Speros, Sara J and MacMurray, Armand J and Kwitek, Anne E and Jacob, Howard J and Lander, Eric S and Lernmark, Åke}}, issn = {{0896-8411}}, language = {{eng}}, number = {{4}}, pages = {{315--324}}, publisher = {{Elsevier}}, series = {{Journal of Autoimmunity}}, title = {{Genetic dissection of lymphopenia from autoimmunity by introgression of mutated Ian5 gene onto the F344 rat.}}, url = {{http://dx.doi.org/10.1016/S0896-8411(03)00138-0}}, doi = {{10.1016/S0896-8411(03)00138-0}}, volume = {{21}}, year = {{2003}}, }