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Detergent Induced Removal of b-Lactoglobulin from Stainless Steel Surfaces at Influenced by Surface Pretreatment

Karlsson, Camilla A-C LU ; Wahlgren, Marie C LU ; Olsson, Claes-Olof A and Trägårdh, Christian A LU (1999) In Journal of Colloid and Interface Science 220. p.471-473
Abstract (Swedish)
The adsorption of β-lactoglobulin to stainless steel and its subsequent removal were followed using in situ null ellipsometry. The influence of the surface pretreatment on the protein removal by the surfactant SDS and by sodium hydroxide was studied. All surfaces were precleaned in strongly alkaline solution. Some surfaces received no further pretreatment, while others were either passivated in nitric acid or plasma-cleaned prior to experiments. Stainless steel surfaces subjected to different surface pretreatments showed considerable differences in cleaning behavior. Cleaning, using NaOH, of surfaces which had been precleaned with alkali only or with plasma resulted in practically complete β-lactoglobulin removal. In contrast, appreciable... (More)
The adsorption of β-lactoglobulin to stainless steel and its subsequent removal were followed using in situ null ellipsometry. The influence of the surface pretreatment on the protein removal by the surfactant SDS and by sodium hydroxide was studied. All surfaces were precleaned in strongly alkaline solution. Some surfaces received no further pretreatment, while others were either passivated in nitric acid or plasma-cleaned prior to experiments. Stainless steel surfaces subjected to different surface pretreatments showed considerable differences in cleaning behavior. Cleaning, using NaOH, of surfaces which had been precleaned with alkali only or with plasma resulted in practically complete β-lactoglobulin removal. In contrast, appreciable amounts of protein remained on passivated stainless steel. Protein removal by SDS was limited and comparable for all three surface pretreatments investigated. Only minor effects on the protein adsorption tendency were observed. The amounts of β-lactoglobulin adsorbed tended to be somewhat lower on the passivated surfaces. (Less)
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author
organization
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Contribution to journal
publication status
published
subject
in
Journal of Colloid and Interface Science
volume
220
pages
3 pages
publisher
Elsevier
external identifiers
  • scopus:0033572359
ISSN
1095-7103
DOI
10.1006/jcis.1999.6503
language
English
LU publication?
yes
id
efbd92f6-865f-4b9c-8ba7-9c812cd07f5a
date added to LUP
2016-04-15 19:28:26
date last changed
2017-07-16 04:24:51
@article{efbd92f6-865f-4b9c-8ba7-9c812cd07f5a,
  abstract     = {The adsorption of β-lactoglobulin to stainless steel and its subsequent removal were followed using in situ null ellipsometry. The influence of the surface pretreatment on the protein removal by the surfactant SDS and by sodium hydroxide was studied. All surfaces were precleaned in strongly alkaline solution. Some surfaces received no further pretreatment, while others were either passivated in nitric acid or plasma-cleaned prior to experiments. Stainless steel surfaces subjected to different surface pretreatments showed considerable differences in cleaning behavior. Cleaning, using NaOH, of surfaces which had been precleaned with alkali only or with plasma resulted in practically complete β-lactoglobulin removal. In contrast, appreciable amounts of protein remained on passivated stainless steel. Protein removal by SDS was limited and comparable for all three surface pretreatments investigated. Only minor effects on the protein adsorption tendency were observed. The amounts of β-lactoglobulin adsorbed tended to be somewhat lower on the passivated surfaces.},
  author       = {Karlsson, Camilla A-C and Wahlgren, Marie C and Olsson, Claes-Olof A and Trägårdh, Christian A},
  issn         = {1095-7103},
  language     = {eng},
  pages        = {471--473},
  publisher    = {Elsevier},
  series       = {Journal of Colloid and Interface Science},
  title        = {Detergent Induced Removal of b-Lactoglobulin from Stainless Steel Surfaces at Influenced by Surface Pretreatment},
  url          = {http://dx.doi.org/10.1006/jcis.1999.6503},
  volume       = {220},
  year         = {1999},
}