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Cystatin C : a new marker of biocompatibility or a good marker for the redistribution of LMW proteins during hemodialysis?

Thysell, Hans LU ; Grubb, Anders LU orcid ; Lindholm, Tore ; Ljunggren, Lennart and Mårtensson, Lena (1988) In ASAIO Transactions 34(3). p.202-204
Abstract

The mechanism(s) behind the larger relative increase of Plasma beta 2 microglobulin (P-beta 2m) than that of Plasma albumin (P-alb) during Cuprophan hemodialysis is disputed. To elucidate this phenomenon P-alb, P-beta 2m (MW 11,800) and Plasma cystatin (P-cC; MW 13,000) an inhibitor of cystein proteinases, were determined before and after a Cuprophan or polysulphone hemodialysis (4-7 hr, QB 200 ml/min) in 30 stable regular dialysis treatment (RDT) patients. Body weight (BW) decreased by 2.5 +/- 1.4% (mean +/- SD). P-alb, P-beta 2m and P-cC increased by 11.4 +/- 14.8%, 15.4 +/- 11.5%, and 22.1 +/- 14.3%, respectively, during Cuprophan dialysis. The relative increase of P-cC was larger than that of P-beta 2m (P less than 0.05) and that of... (More)

The mechanism(s) behind the larger relative increase of Plasma beta 2 microglobulin (P-beta 2m) than that of Plasma albumin (P-alb) during Cuprophan hemodialysis is disputed. To elucidate this phenomenon P-alb, P-beta 2m (MW 11,800) and Plasma cystatin (P-cC; MW 13,000) an inhibitor of cystein proteinases, were determined before and after a Cuprophan or polysulphone hemodialysis (4-7 hr, QB 200 ml/min) in 30 stable regular dialysis treatment (RDT) patients. Body weight (BW) decreased by 2.5 +/- 1.4% (mean +/- SD). P-alb, P-beta 2m and P-cC increased by 11.4 +/- 14.8%, 15.4 +/- 11.5%, and 22.1 +/- 14.3%, respectively, during Cuprophan dialysis. The relative increase of P-cC was larger than that of P-beta 2m (P less than 0.05) and that of P-alb (P less than 0.02). During polysulphone dialysis BW decreased by 4.1 +/- 1.8%. P-alb, P-beta 2m, and P-cC increased almost equally by 28.1 +/- 18, 26.5 +/- 19.2, and 26.8 +/- 14.4%, respectively. These results are hard to interpret. Is the increase in P-cC a new marker of biocompatibility or does it reflect the true shift of low molecular weight (LMW) proteins between the interstitial and the plasma volume during hemodialysis better than P-beta 2m? In vitro studies indicate that small amounts of both Serum beta 2m (S-beta 2m) and Serum cystatin C (S-cC) are adsorbed to or sieved through the Cuprophan membrane, findings which render the kinetics of LMW proteins during hemodialysis still more complex.

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organization
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type
Contribution to journal
publication status
published
subject
keywords
Biocompatible Materials, Blood Proteins, Body Weight, Cellulose/analogs & derivatives, Cystatin C, Cystatins, Humans, Membranes, Artificial, Polymers, Protease Inhibitors/blood, Renal Dialysis, Serum Albumin/analysis, Sulfones, beta 2-Microglobulin/analysis
in
ASAIO Transactions
volume
34
issue
3
pages
202 - 204
publisher
Amercian Society of Artificial Internal Organs
external identifiers
  • pmid:3058174
  • scopus:0023698888
ISSN
0889-7190
language
English
LU publication?
yes
id
f1910299-69f2-47a5-b3a3-be4196e71532
alternative location
https://journals.lww.com/asaiojournal/Abstract/1988/07000/Cystatin_C__A_New_Marker_of_Biocompatibility_or_a.12.aspx
date added to LUP
2021-10-27 08:45:21
date last changed
2024-01-12 02:58:29
@article{f1910299-69f2-47a5-b3a3-be4196e71532,
  abstract     = {{<p>The mechanism(s) behind the larger relative increase of Plasma beta 2 microglobulin (P-beta 2m) than that of Plasma albumin (P-alb) during Cuprophan hemodialysis is disputed. To elucidate this phenomenon P-alb, P-beta 2m (MW 11,800) and Plasma cystatin (P-cC; MW 13,000) an inhibitor of cystein proteinases, were determined before and after a Cuprophan or polysulphone hemodialysis (4-7 hr, QB 200 ml/min) in 30 stable regular dialysis treatment (RDT) patients. Body weight (BW) decreased by 2.5 +/- 1.4% (mean +/- SD). P-alb, P-beta 2m and P-cC increased by 11.4 +/- 14.8%, 15.4 +/- 11.5%, and 22.1 +/- 14.3%, respectively, during Cuprophan dialysis. The relative increase of P-cC was larger than that of P-beta 2m (P less than 0.05) and that of P-alb (P less than 0.02). During polysulphone dialysis BW decreased by 4.1 +/- 1.8%. P-alb, P-beta 2m, and P-cC increased almost equally by 28.1 +/- 18, 26.5 +/- 19.2, and 26.8 +/- 14.4%, respectively. These results are hard to interpret. Is the increase in P-cC a new marker of biocompatibility or does it reflect the true shift of low molecular weight (LMW) proteins between the interstitial and the plasma volume during hemodialysis better than P-beta 2m? In vitro studies indicate that small amounts of both Serum beta 2m (S-beta 2m) and Serum cystatin C (S-cC) are adsorbed to or sieved through the Cuprophan membrane, findings which render the kinetics of LMW proteins during hemodialysis still more complex.</p>}},
  author       = {{Thysell, Hans and Grubb, Anders and Lindholm, Tore and Ljunggren, Lennart and Mårtensson, Lena}},
  issn         = {{0889-7190}},
  keywords     = {{Biocompatible Materials; Blood Proteins; Body Weight; Cellulose/analogs & derivatives; Cystatin C; Cystatins; Humans; Membranes, Artificial; Polymers; Protease Inhibitors/blood; Renal Dialysis; Serum Albumin/analysis; Sulfones; beta 2-Microglobulin/analysis}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{202--204}},
  publisher    = {{Amercian Society of Artificial Internal Organs}},
  series       = {{ASAIO Transactions}},
  title        = {{Cystatin C : a new marker of biocompatibility or a good marker for the redistribution of LMW proteins during hemodialysis?}},
  url          = {{https://journals.lww.com/asaiojournal/Abstract/1988/07000/Cystatin_C__A_New_Marker_of_Biocompatibility_or_a.12.aspx}},
  volume       = {{34}},
  year         = {{1988}},
}