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A new expression vector for production of enzymes in the yeast Saccharomyces (lachancea) kluyveri.

Rasmussen, Anna LU ; Lv, Y ; Schnackerz, Klaus LU and Piskur, Jure LU (2011) In Nucleosides, Nucleotides & Nucleic Acids 30(12). p.1227-1232
Abstract
We overexpressed and purified enzymes involved in the pyrimidine catabolic pathway in the yeast Saccharomyces (Lachancea) kluyveri. A new vector was therefore designed, providing the first specific expression system in Saccharomyces kluyveri. The URC1 gene was overexpressed and a soluble protein obtained and successfully purified using the C-terminally added His-tag. Our system will be used for further studies of the structure and function of the enzymes belonging to the URC pyrimidine degradation pathway.
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Expression vector, Saccharomyces (Lachancea) kluyveri, URC pathway, catabolism of pyrimidines
in
Nucleosides, Nucleotides & Nucleic Acids
volume
30
issue
12
pages
1227 - 1232
publisher
Taylor & Francis
external identifiers
  • wos:000298738500031
  • pmid:22132979
  • scopus:84855904015
ISSN
1525-7770
DOI
10.1080/15257770.2011.603713
language
English
LU publication?
yes
id
f2665c9b-a074-4024-8cd6-c51bfbfc8758 (old id 2274697)
date added to LUP
2016-04-01 11:06:41
date last changed
2022-01-26 05:27:40
@article{f2665c9b-a074-4024-8cd6-c51bfbfc8758,
  abstract     = {{We overexpressed and purified enzymes involved in the pyrimidine catabolic pathway in the yeast Saccharomyces (Lachancea) kluyveri. A new vector was therefore designed, providing the first specific expression system in Saccharomyces kluyveri. The URC1 gene was overexpressed and a soluble protein obtained and successfully purified using the C-terminally added His-tag. Our system will be used for further studies of the structure and function of the enzymes belonging to the URC pyrimidine degradation pathway.}},
  author       = {{Rasmussen, Anna and Lv, Y and Schnackerz, Klaus and Piskur, Jure}},
  issn         = {{1525-7770}},
  keywords     = {{Expression vector; Saccharomyces (Lachancea) kluyveri; URC pathway; catabolism of pyrimidines}},
  language     = {{eng}},
  number       = {{12}},
  pages        = {{1227--1232}},
  publisher    = {{Taylor & Francis}},
  series       = {{Nucleosides, Nucleotides & Nucleic Acids}},
  title        = {{A new expression vector for production of enzymes in the yeast Saccharomyces (lachancea) kluyveri.}},
  url          = {{http://dx.doi.org/10.1080/15257770.2011.603713}},
  doi          = {{10.1080/15257770.2011.603713}},
  volume       = {{30}},
  year         = {{2011}},
}