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Post-thaw viability and functionality of cryopreserved rat fetal brain cells cocultured with Sertoli cells

Cameron, D F; Othberg, A I LU ; Borlongan, C V; Rashed, S; Anton, A; Saporta, S and Sanberg, P R (1997) In Cell Transplantation 6(2). p.9-185
Abstract

Testis-derived Sertoli cells have been used to create an immune "privileged" site outside of the testis to facilitate cell transplantation protocols for diabetes and neurodegenerative diseases. In addition to secreting immunoprotective factors, Sertoli cells also secrete growth and trophic factors that appear to enhance the posttransplantation viability of isolated cells and, likewise, the postthaw viability of isolated, cryopreserved cells. It would be beneficial if Sertoli cells could be cryopreserved with the transplantable cell type without deleterious effects on the cells. This report describes a protocol for the cocryopreservation of rat Sertoli cells with rat ventral mesencephalic neurons, neurons from the lateral and medial... (More)

Testis-derived Sertoli cells have been used to create an immune "privileged" site outside of the testis to facilitate cell transplantation protocols for diabetes and neurodegenerative diseases. In addition to secreting immunoprotective factors, Sertoli cells also secrete growth and trophic factors that appear to enhance the posttransplantation viability of isolated cells and, likewise, the postthaw viability of isolated, cryopreserved cells. It would be beneficial if Sertoli cells could be cryopreserved with the transplantable cell type without deleterious effects on the cells. This report describes a protocol for the cocryopreservation of rat Sertoli cells with rat ventral mesencephalic neurons, neurons from the lateral and medial ganglionic eminences and the hNT neuron cell line, and reports on the effects of Sertoli cells on the the postthaw viability of these neurons. Results of trypan blue exclusion analysis indicated that the presence of Sertoli cells did not deleteriously effect cryopreserved neurons and may improve their postthaw recoverability and viability in general. Specifically, results of the tyrosine hydroxylase immunostaining showed that Sertoli cells significantly enhance the postthaw viability of ventral mesencephalic dopaminergic cells in vitro.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Animals, Brain, Cell Survival, Coculture Techniques, Cryopreservation, Culture Media, Conditioned, Fetus, Freezing, Male, Neurons, Rats, Rats, Sprague-Dawley, Sertoli Cells, Tyrosine 3-Monooxygenase, Journal Article, Research Support, Non-U.S. Gov't
in
Cell Transplantation
volume
6
issue
2
pages
5 pages
publisher
Cognizant Communication Corporation
external identifiers
  • scopus:0030949885
ISSN
0963-6897
DOI
10.1016/S0963-6897(97)00022-5
language
English
LU publication?
no
id
f27bde89-e9f0-4881-a270-f21dd351985d
date added to LUP
2016-12-11 10:06:16
date last changed
2017-01-01 08:42:56
@article{f27bde89-e9f0-4881-a270-f21dd351985d,
  abstract     = {<p>Testis-derived Sertoli cells have been used to create an immune "privileged" site outside of the testis to facilitate cell transplantation protocols for diabetes and neurodegenerative diseases. In addition to secreting immunoprotective factors, Sertoli cells also secrete growth and trophic factors that appear to enhance the posttransplantation viability of isolated cells and, likewise, the postthaw viability of isolated, cryopreserved cells. It would be beneficial if Sertoli cells could be cryopreserved with the transplantable cell type without deleterious effects on the cells. This report describes a protocol for the cocryopreservation of rat Sertoli cells with rat ventral mesencephalic neurons, neurons from the lateral and medial ganglionic eminences and the hNT neuron cell line, and reports on the effects of Sertoli cells on the the postthaw viability of these neurons. Results of trypan blue exclusion analysis indicated that the presence of Sertoli cells did not deleteriously effect cryopreserved neurons and may improve their postthaw recoverability and viability in general. Specifically, results of the tyrosine hydroxylase immunostaining showed that Sertoli cells significantly enhance the postthaw viability of ventral mesencephalic dopaminergic cells in vitro.</p>},
  author       = {Cameron, D F and Othberg, A I and Borlongan, C V and Rashed, S and Anton, A and Saporta, S and Sanberg, P R},
  issn         = {0963-6897},
  keyword      = {Animals,Brain,Cell Survival,Coculture Techniques,Cryopreservation,Culture Media, Conditioned,Fetus,Freezing,Male,Neurons,Rats,Rats, Sprague-Dawley,Sertoli Cells,Tyrosine 3-Monooxygenase,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  month        = {03},
  number       = {2},
  pages        = {9--185},
  publisher    = {Cognizant Communication Corporation},
  series       = {Cell Transplantation},
  title        = {Post-thaw viability and functionality of cryopreserved rat fetal brain cells cocultured with Sertoli cells},
  url          = {http://dx.doi.org/10.1016/S0963-6897(97)00022-5},
  volume       = {6},
  year         = {1997},
}