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Time-resolved fluorescence imaging (TRFI) for direct immunofluorescence of PSA and alpha-1-antichymotrypsin in prostatic tissue sections

Bjartell, A. LU ; Siivola, P. ; Hulkko, S. ; Pettersson, K. ; Rundt, K. ; Lilja, H. LU orcid and Lövgren, T. (1999) In Prostate Cancer and Prostatic Diseases 2(3). p.140-147
Abstract

We have developed a direct immunofluorescence technique utilising chelates of the lanthanide ions europium and terbium conjugated to monoclonal IgGs (Mabs) against prostate-specific antigen (PSA) and alpha-1- antichymotrypsin (ACT) for the detection and quantification on the same tissue section. Strong signals without disturbance from tissue autofluorescence were demonstrated in paraffin sections of ten benign and six malignant prostate tissue specimens. The signal intensity increased linearly with the amount of labelled Mab until epitope saturation began. The highest concentrations of bound IgG in tissue sections were 27.3 fmol/pixel for ACT and 7.2 for PSA. Time-resolved fluorescence imaging (TRFI) offers an attractive method for... (More)

We have developed a direct immunofluorescence technique utilising chelates of the lanthanide ions europium and terbium conjugated to monoclonal IgGs (Mabs) against prostate-specific antigen (PSA) and alpha-1- antichymotrypsin (ACT) for the detection and quantification on the same tissue section. Strong signals without disturbance from tissue autofluorescence were demonstrated in paraffin sections of ten benign and six malignant prostate tissue specimens. The signal intensity increased linearly with the amount of labelled Mab until epitope saturation began. The highest concentrations of bound IgG in tissue sections were 27.3 fmol/pixel for ACT and 7.2 for PSA. Time-resolved fluorescence imaging (TRFI) offers an attractive method for histochemical studies based on specific and quantitative detection of fluorescent lanthanide chelates.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Europium, Image analysis, Immunohistochemistry, Lanthanide chelates, Prostate cancer, Terbium
in
Prostate Cancer and Prostatic Diseases
volume
2
issue
3
pages
8 pages
publisher
Nature Publishing Group
external identifiers
  • scopus:0032858373
ISSN
1365-7852
DOI
10.1038/sj.pcan.4500307
language
English
LU publication?
yes
id
f364d898-61a3-41c0-9bb8-4c8081601383
date added to LUP
2022-12-06 17:45:08
date last changed
2023-01-06 19:37:17
@article{f364d898-61a3-41c0-9bb8-4c8081601383,
  abstract     = {{<p>We have developed a direct immunofluorescence technique utilising chelates of the lanthanide ions europium and terbium conjugated to monoclonal IgGs (Mabs) against prostate-specific antigen (PSA) and alpha-1- antichymotrypsin (ACT) for the detection and quantification on the same tissue section. Strong signals without disturbance from tissue autofluorescence were demonstrated in paraffin sections of ten benign and six malignant prostate tissue specimens. The signal intensity increased linearly with the amount of labelled Mab until epitope saturation began. The highest concentrations of bound IgG in tissue sections were 27.3 fmol/pixel for ACT and 7.2 for PSA. Time-resolved fluorescence imaging (TRFI) offers an attractive method for histochemical studies based on specific and quantitative detection of fluorescent lanthanide chelates.</p>}},
  author       = {{Bjartell, A. and Siivola, P. and Hulkko, S. and Pettersson, K. and Rundt, K. and Lilja, H. and Lövgren, T.}},
  issn         = {{1365-7852}},
  keywords     = {{Europium; Image analysis; Immunohistochemistry; Lanthanide chelates; Prostate cancer; Terbium}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{140--147}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Prostate Cancer and Prostatic Diseases}},
  title        = {{Time-resolved fluorescence imaging (TRFI) for direct immunofluorescence of PSA and alpha-1-antichymotrypsin in prostatic tissue sections}},
  url          = {{http://dx.doi.org/10.1038/sj.pcan.4500307}},
  doi          = {{10.1038/sj.pcan.4500307}},
  volume       = {{2}},
  year         = {{1999}},
}