The triolein/aqueous interface and lipase activity studied by spectroscopic ellipsometry and coarse grained simulations
(2018) In Chemistry and Physics of Lipids 211. p.37-43- Abstract
In spite of the importance of the triglyceride aqueous interface for processes like emulsification, surfactant interactions and lipase activity, relatively little is known about this interface compared to that between alkanes and water. Here, the contact between triolein and water was investigated in terms of water inclusion in the oil phase and orientation of the molecules at the interface. Coarse grained models of triglycerides in contact with water were constructed and correlated with experimental results of the changes in thickness and refractive index, obtained using spectroscopic ellipsometry of spin-coated triolein films. The topography of the layer was revealed by atomic force microscopy. Dry triolein and a triolein sample after... (More)
In spite of the importance of the triglyceride aqueous interface for processes like emulsification, surfactant interactions and lipase activity, relatively little is known about this interface compared to that between alkanes and water. Here, the contact between triolein and water was investigated in terms of water inclusion in the oil phase and orientation of the molecules at the interface. Coarse grained models of triglycerides in contact with water were constructed and correlated with experimental results of the changes in thickness and refractive index, obtained using spectroscopic ellipsometry of spin-coated triolein films. The topography of the layer was revealed by atomic force microscopy. Dry triolein and a triolein sample after equilibration with water were also compared structurally using small-angle X-ray scattering. Additionally, the kinetics of adsorption/activity of three different variants of the Thermomyces lanuginosus lipase (TLL) were investigated. The results show that uptake of water in the triolein phase leads to increase in thickness of the layer. The observed increase of thickness was further enhanced by an active lipase but reduced when an inactive mutant of the enzyme was applied.
(Less)
- author
- Stamm, Arne ; Svendsen, Allan ; Skjold-Jørgensen, Jakob ; Vissing, Thomas ; Berts, Ida and Nylander, Tommy LU
- organization
- publishing date
- 2018
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Coarse graining simulations, Lipase activity, Oil phase hydration, Small-angle x-ray scattering, Spectroscopic ellipsometry, Thermomyces lanuginosus lipase, Triolein
- in
- Chemistry and Physics of Lipids
- volume
- 211
- pages
- 7 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:85034650250
- pmid:29129569
- ISSN
- 0009-3084
- DOI
- 10.1016/j.chemphyslip.2017.10.011
- language
- English
- LU publication?
- yes
- id
- f393038f-4691-47c3-8e1f-2219d2195b88
- date added to LUP
- 2017-12-11 12:25:31
- date last changed
- 2024-04-29 01:40:18
@article{f393038f-4691-47c3-8e1f-2219d2195b88,
abstract = {{<p>In spite of the importance of the triglyceride aqueous interface for processes like emulsification, surfactant interactions and lipase activity, relatively little is known about this interface compared to that between alkanes and water. Here, the contact between triolein and water was investigated in terms of water inclusion in the oil phase and orientation of the molecules at the interface. Coarse grained models of triglycerides in contact with water were constructed and correlated with experimental results of the changes in thickness and refractive index, obtained using spectroscopic ellipsometry of spin-coated triolein films. The topography of the layer was revealed by atomic force microscopy. Dry triolein and a triolein sample after equilibration with water were also compared structurally using small-angle X-ray scattering. Additionally, the kinetics of adsorption/activity of three different variants of the Thermomyces lanuginosus lipase (TLL) were investigated. The results show that uptake of water in the triolein phase leads to increase in thickness of the layer. The observed increase of thickness was further enhanced by an active lipase but reduced when an inactive mutant of the enzyme was applied.</p>}},
author = {{Stamm, Arne and Svendsen, Allan and Skjold-Jørgensen, Jakob and Vissing, Thomas and Berts, Ida and Nylander, Tommy}},
issn = {{0009-3084}},
keywords = {{Coarse graining simulations; Lipase activity; Oil phase hydration; Small-angle x-ray scattering; Spectroscopic ellipsometry; Thermomyces lanuginosus lipase; Triolein}},
language = {{eng}},
pages = {{37--43}},
publisher = {{Elsevier}},
series = {{Chemistry and Physics of Lipids}},
title = {{The triolein/aqueous interface and lipase activity studied by spectroscopic ellipsometry and coarse grained simulations}},
url = {{http://dx.doi.org/10.1016/j.chemphyslip.2017.10.011}},
doi = {{10.1016/j.chemphyslip.2017.10.011}},
volume = {{211}},
year = {{2018}},
}