Advanced

The triolein/aqueous interface and lipase activity studied by spectroscopic ellipsometry and coarse grained simulations

Stamm, Arne; Svendsen, Allan; Skjold-Jørgensen, Jakob; Vissing, Thomas; Berts, Ida and Nylander, Tommy LU (2017) In Chemistry and Physics of Lipids
Abstract

In spite of the importance of the triglyceride aqueous interface for processes like emulsification, surfactant interactions and lipase activity, relatively little is known about this interface compared to that between alkanes and water. Here, the contact between triolein and water was investigated in terms of water inclusion in the oil phase and orientation of the molecules at the interface. Coarse grained models of triglycerides in contact with water were constructed and correlated with experimental results of the changes in thickness and refractive index, obtained using spectroscopic ellipsometry of spin-coated triolein films. The topography of the layer was revealed by atomic force microscopy. Dry triolein and a triolein sample after... (More)

In spite of the importance of the triglyceride aqueous interface for processes like emulsification, surfactant interactions and lipase activity, relatively little is known about this interface compared to that between alkanes and water. Here, the contact between triolein and water was investigated in terms of water inclusion in the oil phase and orientation of the molecules at the interface. Coarse grained models of triglycerides in contact with water were constructed and correlated with experimental results of the changes in thickness and refractive index, obtained using spectroscopic ellipsometry of spin-coated triolein films. The topography of the layer was revealed by atomic force microscopy. Dry triolein and a triolein sample after equilibration with water were also compared structurally using small-angle X-ray scattering. Additionally, the kinetics of adsorption/activity of three different variants of the Thermomyces lanuginosus lipase (TLL) were investigated. The results show that uptake of water in the triolein phase leads to increase in thickness of the layer. The observed increase of thickness was further enhanced by an active lipase but reduced when an inactive mutant of the enzyme was applied.

(Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
Coarse graining simulations, Lipase activity, Oil phase hydration, Small-angle x-ray scattering, Spectroscopic ellipsometry, Thermomyces lanuginosus lipase, Triolein
in
Chemistry and Physics of Lipids
publisher
Elsevier
external identifiers
  • scopus:85034650250
ISSN
0009-3084
DOI
10.1016/j.chemphyslip.2017.10.011
language
English
LU publication?
yes
id
f393038f-4691-47c3-8e1f-2219d2195b88
date added to LUP
2017-12-11 12:25:31
date last changed
2018-01-07 12:28:08
@article{f393038f-4691-47c3-8e1f-2219d2195b88,
  abstract     = {<p>In spite of the importance of the triglyceride aqueous interface for processes like emulsification, surfactant interactions and lipase activity, relatively little is known about this interface compared to that between alkanes and water. Here, the contact between triolein and water was investigated in terms of water inclusion in the oil phase and orientation of the molecules at the interface. Coarse grained models of triglycerides in contact with water were constructed and correlated with experimental results of the changes in thickness and refractive index, obtained using spectroscopic ellipsometry of spin-coated triolein films. The topography of the layer was revealed by atomic force microscopy. Dry triolein and a triolein sample after equilibration with water were also compared structurally using small-angle X-ray scattering. Additionally, the kinetics of adsorption/activity of three different variants of the Thermomyces lanuginosus lipase (TLL) were investigated. The results show that uptake of water in the triolein phase leads to increase in thickness of the layer. The observed increase of thickness was further enhanced by an active lipase but reduced when an inactive mutant of the enzyme was applied.</p>},
  author       = {Stamm, Arne and Svendsen, Allan and Skjold-Jørgensen, Jakob and Vissing, Thomas and Berts, Ida and Nylander, Tommy},
  issn         = {0009-3084},
  keyword      = {Coarse graining simulations,Lipase activity,Oil phase hydration,Small-angle x-ray scattering,Spectroscopic ellipsometry,Thermomyces lanuginosus lipase,Triolein},
  language     = {eng},
  month        = {11},
  publisher    = {Elsevier},
  series       = {Chemistry and Physics of Lipids},
  title        = {The triolein/aqueous interface and lipase activity studied by spectroscopic ellipsometry and coarse grained simulations},
  url          = {http://dx.doi.org/10.1016/j.chemphyslip.2017.10.011},
  year         = {2017},
}