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Atorvastatin activates PPAR-γ and attenuates the inflammatory response in human monocytes

Grip, Olof LU ; Janciauskiene, S. LU and Lindgren, S. LU (2002) In Inflammation Research 51(2). p.58-62
Abstract

Objective: To investigate the ability of statins to activate the nuclear receptor peroxisome proliferator-activated receptor gamma (PPAR-γ) in primary human monocytes in culture. Materials and methods: Human peripheral monocytes were incubated with atorvastatin (0.1-10 μmol/1) for up to 24 hours. PPAR-γ expression was analysed by electrophoretic mobility shift assay. Pro-inflammatory cytokines were measured by enzyme-linked immunosorbent assays, and oxygen consumption was determined polarographically with a Clark-type oxygen electrode. Results: We found that atorvastatin activates PPAR-γ and inhibits the production of tumour necrosis factor-alpha up to 38% (p < 0.05), monocyte chemoattractant protein-1 up to 85% (p < 0.05), and... (More)

Objective: To investigate the ability of statins to activate the nuclear receptor peroxisome proliferator-activated receptor gamma (PPAR-γ) in primary human monocytes in culture. Materials and methods: Human peripheral monocytes were incubated with atorvastatin (0.1-10 μmol/1) for up to 24 hours. PPAR-γ expression was analysed by electrophoretic mobility shift assay. Pro-inflammatory cytokines were measured by enzyme-linked immunosorbent assays, and oxygen consumption was determined polarographically with a Clark-type oxygen electrode. Results: We found that atorvastatin activates PPAR-γ and inhibits the production of tumour necrosis factor-alpha up to 38% (p < 0.05), monocyte chemoattractant protein-1 up to 85% (p < 0.05), and gelatinase B up to 73% (p < 0.05), in a concentration-dependent manner. Moreover, atorvastatin shows concentration-dependent inhibition of cellular oxygen consumption up to 41%. Conclusions: These findings contribute to the growing knowledge of the anti-inflammatory effects of statins, and have led us to the suggestion that statins may control inflammatory responses by the regulation of intracellular lipid homeostasis.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Atorvastatin, Inflammation, Monocytes, PPAR-γ
in
Inflammation Research
volume
51
issue
2
pages
58 - 62
publisher
Birkhaüser
external identifiers
  • scopus:0036186165
  • pmid:11926313
ISSN
1023-3830
DOI
10.1007/BF02684000
language
English
LU publication?
yes
id
f3aad159-89d5-4708-b006-68530bfc09f3
date added to LUP
2019-08-07 13:29:27
date last changed
2019-12-03 02:17:28
@article{f3aad159-89d5-4708-b006-68530bfc09f3,
  abstract     = {<p>Objective: To investigate the ability of statins to activate the nuclear receptor peroxisome proliferator-activated receptor gamma (PPAR-γ) in primary human monocytes in culture. Materials and methods: Human peripheral monocytes were incubated with atorvastatin (0.1-10 μmol/1) for up to 24 hours. PPAR-γ expression was analysed by electrophoretic mobility shift assay. Pro-inflammatory cytokines were measured by enzyme-linked immunosorbent assays, and oxygen consumption was determined polarographically with a Clark-type oxygen electrode. Results: We found that atorvastatin activates PPAR-γ and inhibits the production of tumour necrosis factor-alpha up to 38% (p &lt; 0.05), monocyte chemoattractant protein-1 up to 85% (p &lt; 0.05), and gelatinase B up to 73% (p &lt; 0.05), in a concentration-dependent manner. Moreover, atorvastatin shows concentration-dependent inhibition of cellular oxygen consumption up to 41%. Conclusions: These findings contribute to the growing knowledge of the anti-inflammatory effects of statins, and have led us to the suggestion that statins may control inflammatory responses by the regulation of intracellular lipid homeostasis.</p>},
  author       = {Grip, Olof and Janciauskiene, S. and Lindgren, S.},
  issn         = {1023-3830},
  language     = {eng},
  number       = {2},
  pages        = {58--62},
  publisher    = {Birkhaüser},
  series       = {Inflammation Research},
  title        = {Atorvastatin activates PPAR-γ and attenuates the inflammatory response in human monocytes},
  url          = {http://dx.doi.org/10.1007/BF02684000},
  doi          = {10.1007/BF02684000},
  volume       = {51},
  year         = {2002},
}