Transformation of the nematode-trapping fungus Arthrobotrys oligospora
(1999) In FEMS Microbiology Letters 173(1). p.111-116- Abstract
The nematode-trapping fungus Arthrobotrys oligospora was transformed to hygromycin resistance using the hygromycin-B phosphotransferase gene from Escherichia coli under the control of various heterologous fungal promoters. Plasmid DNA was introduced into fungal protoplasts by polyethylene glycol/CaCl2 treatment. Transformation frequencies varied between 1-6 transformants per μg DNA. Seven out of 13 integration events analyzed from transformants were single copy integrations, whereas the remaining were multiple and more complex integrations. The addition of restriction enzymes during transformations increased the frequency of single copy integrations. Co-transformation, using the E. coli uidA gene encoding the β-glucuronidase... (More)
The nematode-trapping fungus Arthrobotrys oligospora was transformed to hygromycin resistance using the hygromycin-B phosphotransferase gene from Escherichia coli under the control of various heterologous fungal promoters. Plasmid DNA was introduced into fungal protoplasts by polyethylene glycol/CaCl2 treatment. Transformation frequencies varied between 1-6 transformants per μg DNA. Seven out of 13 integration events analyzed from transformants were single copy integrations, whereas the remaining were multiple and more complex integrations. The addition of restriction enzymes during transformations increased the frequency of single copy integrations. Co-transformation, using the E. coli uidA gene encoding the β-glucuronidase reporter gene under the control of an Aspergillus nidulans promoter, occurred at frequencies of up to 63%. Copyright (C) 1999 Federation of European Microbiological Societies.
(Less)
- author
- Tunlid, A. LU ; Åhman, J. LU and Oliver, R. P.
- organization
- publishing date
- 1999-04-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- β-Glucuronidase reporter gene, Hygromycin resistance, Nematode-trapping fungi, Restriction mediated integration, Transformation, Biologi
- in
- FEMS Microbiology Letters
- volume
- 173
- issue
- 1
- pages
- 111 - 116
- publisher
- Oxford University Press
- external identifiers
-
- pmid:10220888
- scopus:0033046242
- ISSN
- 0378-1097
- DOI
- 10.1016/S0378-1097(99)00059-2
- language
- English
- LU publication?
- yes
- id
- f3bb6326-d184-4923-9458-79859eb57129
- date added to LUP
- 2019-10-23 16:53:47
- date last changed
- 2024-06-13 06:03:47
@article{f3bb6326-d184-4923-9458-79859eb57129, abstract = {{<p>The nematode-trapping fungus Arthrobotrys oligospora was transformed to hygromycin resistance using the hygromycin-B phosphotransferase gene from Escherichia coli under the control of various heterologous fungal promoters. Plasmid DNA was introduced into fungal protoplasts by polyethylene glycol/CaCl<sub>2</sub> treatment. Transformation frequencies varied between 1-6 transformants per μg DNA. Seven out of 13 integration events analyzed from transformants were single copy integrations, whereas the remaining were multiple and more complex integrations. The addition of restriction enzymes during transformations increased the frequency of single copy integrations. Co-transformation, using the E. coli uidA gene encoding the β-glucuronidase reporter gene under the control of an Aspergillus nidulans promoter, occurred at frequencies of up to 63%. Copyright (C) 1999 Federation of European Microbiological Societies.</p>}}, author = {{Tunlid, A. and Åhman, J. and Oliver, R. P.}}, issn = {{0378-1097}}, keywords = {{β-Glucuronidase reporter gene; Hygromycin resistance; Nematode-trapping fungi; Restriction mediated integration; Transformation; Biologi}}, language = {{eng}}, month = {{04}}, number = {{1}}, pages = {{111--116}}, publisher = {{Oxford University Press}}, series = {{FEMS Microbiology Letters}}, title = {{Transformation of the nematode-trapping fungus Arthrobotrys oligospora}}, url = {{http://dx.doi.org/10.1016/S0378-1097(99)00059-2}}, doi = {{10.1016/S0378-1097(99)00059-2}}, volume = {{173}}, year = {{1999}}, }