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Transformation of the nematode-trapping fungus Arthrobotrys oligospora

Tunlid, A. LU ; Åhman, J. LU and Oliver, R. P. (1999) In FEMS Microbiology Letters 173(1). p.111-116
Abstract

The nematode-trapping fungus Arthrobotrys oligospora was transformed to hygromycin resistance using the hygromycin-B phosphotransferase gene from Escherichia coli under the control of various heterologous fungal promoters. Plasmid DNA was introduced into fungal protoplasts by polyethylene glycol/CaCl2 treatment. Transformation frequencies varied between 1-6 transformants per μg DNA. Seven out of 13 integration events analyzed from transformants were single copy integrations, whereas the remaining were multiple and more complex integrations. The addition of restriction enzymes during transformations increased the frequency of single copy integrations. Co-transformation, using the E. coli uidA gene encoding the β-glucuronidase... (More)

The nematode-trapping fungus Arthrobotrys oligospora was transformed to hygromycin resistance using the hygromycin-B phosphotransferase gene from Escherichia coli under the control of various heterologous fungal promoters. Plasmid DNA was introduced into fungal protoplasts by polyethylene glycol/CaCl2 treatment. Transformation frequencies varied between 1-6 transformants per μg DNA. Seven out of 13 integration events analyzed from transformants were single copy integrations, whereas the remaining were multiple and more complex integrations. The addition of restriction enzymes during transformations increased the frequency of single copy integrations. Co-transformation, using the E. coli uidA gene encoding the β-glucuronidase reporter gene under the control of an Aspergillus nidulans promoter, occurred at frequencies of up to 63%. Copyright (C) 1999 Federation of European Microbiological Societies.

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type
Contribution to journal
publication status
published
subject
keywords
β-Glucuronidase reporter gene, Hygromycin resistance, Nematode-trapping fungi, Restriction mediated integration, Transformation, Biologi
in
FEMS Microbiology Letters
volume
173
issue
1
pages
111 - 116
publisher
Oxford University Press
external identifiers
  • scopus:0033046242
  • pmid:10220888
ISSN
0378-1097
DOI
10.1016/S0378-1097(99)00059-2
language
English
LU publication?
yes
id
f3bb6326-d184-4923-9458-79859eb57129
date added to LUP
2019-10-23 16:53:47
date last changed
2024-06-13 06:03:47
@article{f3bb6326-d184-4923-9458-79859eb57129,
  abstract     = {{<p>The nematode-trapping fungus Arthrobotrys oligospora was transformed to hygromycin resistance using the hygromycin-B phosphotransferase gene from Escherichia coli under the control of various heterologous fungal promoters. Plasmid DNA was introduced into fungal protoplasts by polyethylene glycol/CaCl<sub>2</sub> treatment. Transformation frequencies varied between 1-6 transformants per μg DNA. Seven out of 13 integration events analyzed from transformants were single copy integrations, whereas the remaining were multiple and more complex integrations. The addition of restriction enzymes during transformations increased the frequency of single copy integrations. Co-transformation, using the E. coli uidA gene encoding the β-glucuronidase reporter gene under the control of an Aspergillus nidulans promoter, occurred at frequencies of up to 63%. Copyright (C) 1999 Federation of European Microbiological Societies.</p>}},
  author       = {{Tunlid, A. and Åhman, J. and Oliver, R. P.}},
  issn         = {{0378-1097}},
  keywords     = {{β-Glucuronidase reporter gene; Hygromycin resistance; Nematode-trapping fungi; Restriction mediated integration; Transformation; Biologi}},
  language     = {{eng}},
  month        = {{04}},
  number       = {{1}},
  pages        = {{111--116}},
  publisher    = {{Oxford University Press}},
  series       = {{FEMS Microbiology Letters}},
  title        = {{Transformation of the nematode-trapping fungus Arthrobotrys oligospora}},
  url          = {{http://dx.doi.org/10.1016/S0378-1097(99)00059-2}},
  doi          = {{10.1016/S0378-1097(99)00059-2}},
  volume       = {{173}},
  year         = {{1999}},
}