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Cell sorting using electrokinetic deterministic lateral displacement

Ho, Bao D. LU ; Beech, Jason P. LU and Tegenfeldt, Jonas O. LU orcid (2020) In Micromachines 12(1).
Abstract

We show that by combining deterministic lateral displacement (DLD) with electrokinetics, it is possible to sort cells based on differences in their membrane and/or internal structures. Using heat to deactivate cells, which change their viability and structure, we then demonstrate sorting of a mixture of viable and non-viable cells for two different cell types. For Escherichia coli, the size change due to deactivation is insufficient to allow size-based DLD separation. Our method instead leverages the considerable change in zeta potential to achieve separation at low frequency. Conversely, for Saccharomyces cerevisiae (Baker’s yeast) the heat treatment does not result in any significant change of zeta potential. Instead, we perform the... (More)

We show that by combining deterministic lateral displacement (DLD) with electrokinetics, it is possible to sort cells based on differences in their membrane and/or internal structures. Using heat to deactivate cells, which change their viability and structure, we then demonstrate sorting of a mixture of viable and non-viable cells for two different cell types. For Escherichia coli, the size change due to deactivation is insufficient to allow size-based DLD separation. Our method instead leverages the considerable change in zeta potential to achieve separation at low frequency. Conversely, for Saccharomyces cerevisiae (Baker’s yeast) the heat treatment does not result in any significant change of zeta potential. Instead, we perform the sorting at higher frequency and utilize what we believe is a change in dielectrophoretic mobility for the separation. We expect our work to form a basis for the development of simple, low-cost, continuous label-free methods that can separate cells and bioparticles based on their intrinsic properties.

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Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Charge-based separation, Dielectrophoresis, Electrokinetic deterministic lateral displacement
in
Micromachines
volume
12
issue
1
article number
30
pages
14 pages
publisher
MDPI AG
external identifiers
  • pmid:33396630
  • scopus:85099513085
ISSN
2072-666X
DOI
10.3390/mi12010030
language
English
LU publication?
yes
id
f3d719f8-bc34-4865-a64d-bfa3b38d547f
date added to LUP
2021-01-29 11:27:12
date last changed
2024-11-14 23:20:00
@article{f3d719f8-bc34-4865-a64d-bfa3b38d547f,
  abstract     = {{<p>We show that by combining deterministic lateral displacement (DLD) with electrokinetics, it is possible to sort cells based on differences in their membrane and/or internal structures. Using heat to deactivate cells, which change their viability and structure, we then demonstrate sorting of a mixture of viable and non-viable cells for two different cell types. For Escherichia coli, the size change due to deactivation is insufficient to allow size-based DLD separation. Our method instead leverages the considerable change in zeta potential to achieve separation at low frequency. Conversely, for Saccharomyces cerevisiae (Baker’s yeast) the heat treatment does not result in any significant change of zeta potential. Instead, we perform the sorting at higher frequency and utilize what we believe is a change in dielectrophoretic mobility for the separation. We expect our work to form a basis for the development of simple, low-cost, continuous label-free methods that can separate cells and bioparticles based on their intrinsic properties.</p>}},
  author       = {{Ho, Bao D. and Beech, Jason P. and Tegenfeldt, Jonas O.}},
  issn         = {{2072-666X}},
  keywords     = {{Charge-based separation; Dielectrophoresis; Electrokinetic deterministic lateral displacement}},
  language     = {{eng}},
  number       = {{1}},
  publisher    = {{MDPI AG}},
  series       = {{Micromachines}},
  title        = {{Cell sorting using electrokinetic deterministic lateral displacement}},
  url          = {{http://dx.doi.org/10.3390/mi12010030}},
  doi          = {{10.3390/mi12010030}},
  volume       = {{12}},
  year         = {{2020}},
}