Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Verification of Pathologic ECV Estimated with Clinical T1 Mapping Methods Using an Isotope-based Reference

Nordlund, David LU ; Kopic, Sascha LU ; Jablonowski, Robert LU ; Xanthis, Christos LU ; Bidhult, Sebastian LU ; Berg, Jonathan LU orcid ; Kanski, Mikael LU ; Chow, Kelvin ; Aletras, Anthony H. LU orcid and Arheden, Håkan LU (2025) In Radiology: Cardiothoracic Imaging 7(4).
Abstract

Purpose: To verify the accuracy of clinically used cardiac MRI T1 mapping sequences for measuring extracellular volume fraction (ECV) throughout the spectrum of pathologic values using an independent reference standard. Materials and Methods: Acute myocardial ischemia was induced in six pigs using endovascular balloon occlusion in the left anterior descending artery between October 2018 and November 2018. After 7 days of reperfusion, ECV was measured in vivo using four sequences: Three modified Look-Locker inversion recovery (MOLLI) and one saturation recovery single-shot acquisition (SASHA) sequences. ECV was also measured using ex vivo SPECT following injection of technetium 99m diethylenetriamine pentaacetic acid. ECV was calculated... (More)

Purpose: To verify the accuracy of clinically used cardiac MRI T1 mapping sequences for measuring extracellular volume fraction (ECV) throughout the spectrum of pathologic values using an independent reference standard. Materials and Methods: Acute myocardial ischemia was induced in six pigs using endovascular balloon occlusion in the left anterior descending artery between October 2018 and November 2018. After 7 days of reperfusion, ECV was measured in vivo using four sequences: Three modified Look-Locker inversion recovery (MOLLI) and one saturation recovery single-shot acquisition (SASHA) sequences. ECV was also measured using ex vivo SPECT following injection of technetium 99m diethylenetriamine pentaacetic acid. ECV was calculated for corresponding regions of interest of necrosis on cardiac MR and SPECT images and in myocardium remote from injury. Mixed model analysis was used to test for correlation, while the paired t test was used to test for differences in T1 and ECV. Results: ECV measurements with all T1 mapping sequences showed high correlation to the reference standard (r2range, 0.71–0.99). Measurements with MOLLI 5(3)3 and MOLLI 5s(3s)3s overestimated ECV in the infarct region compared with the reference standard (bias ± 2 SDs: 8.2% ± 8.5 and 4.9% ± 8.7; P =.005 and P =.04, respectively). All sequences showed overestimation of ECV in the remote region (range of bias, 3%–14% points, all MOLLI P values ≤.001, SASHA P =.04). Conclusion: This study verified the accuracy of clinically used T1 mapping sequences in measuring ECV in absolute values over a spectrum of pathologic values, using an independent radioisotope-based three-dimensionally acquired reference standard.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Animal Studies, Cardiac, Edema, Imaging Sequences, Ischemia/Infarction, MR Imaging
in
Radiology: Cardiothoracic Imaging
volume
7
issue
4
article number
e240441
external identifiers
  • pmid:40772845
  • scopus:105018502181
DOI
10.1148/ryct.240441
language
English
LU publication?
yes
additional info
Publisher Copyright: © 2025, Radiological Society of North America Inc. All rights reserved.
id
f41c0f26-7af0-4baf-b962-27c08266ece8
date added to LUP
2025-12-19 10:59:54
date last changed
2025-12-20 03:00:24
@article{f41c0f26-7af0-4baf-b962-27c08266ece8,
  abstract     = {{<p>Purpose: To verify the accuracy of clinically used cardiac MRI T1 mapping sequences for measuring extracellular volume fraction (ECV) throughout the spectrum of pathologic values using an independent reference standard. Materials and Methods: Acute myocardial ischemia was induced in six pigs using endovascular balloon occlusion in the left anterior descending artery between October 2018 and November 2018. After 7 days of reperfusion, ECV was measured in vivo using four sequences: Three modified Look-Locker inversion recovery (MOLLI) and one saturation recovery single-shot acquisition (SASHA) sequences. ECV was also measured using ex vivo SPECT following injection of technetium 99m diethylenetriamine pentaacetic acid. ECV was calculated for corresponding regions of interest of necrosis on cardiac MR and SPECT images and in myocardium remote from injury. Mixed model analysis was used to test for correlation, while the paired t test was used to test for differences in T1 and ECV. Results: ECV measurements with all T1 mapping sequences showed high correlation to the reference standard (r<sup>2</sup>range, 0.71–0.99). Measurements with MOLLI 5(3)3 and MOLLI 5s(3s)3s overestimated ECV in the infarct region compared with the reference standard (bias ± 2 SDs: 8.2% ± 8.5 and 4.9% ± 8.7; P =.005 and P =.04, respectively). All sequences showed overestimation of ECV in the remote region (range of bias, 3%–14% points, all MOLLI P values ≤.001, SASHA P =.04). Conclusion: This study verified the accuracy of clinically used T1 mapping sequences in measuring ECV in absolute values over a spectrum of pathologic values, using an independent radioisotope-based three-dimensionally acquired reference standard.</p>}},
  author       = {{Nordlund, David and Kopic, Sascha and Jablonowski, Robert and Xanthis, Christos and Bidhult, Sebastian and Berg, Jonathan and Kanski, Mikael and Chow, Kelvin and Aletras, Anthony H. and Arheden, Håkan}},
  keywords     = {{Animal Studies; Cardiac; Edema; Imaging Sequences; Ischemia/Infarction; MR Imaging}},
  language     = {{eng}},
  number       = {{4}},
  series       = {{Radiology: Cardiothoracic Imaging}},
  title        = {{Verification of Pathologic ECV Estimated with Clinical T1 Mapping Methods Using an Isotope-based Reference}},
  url          = {{http://dx.doi.org/10.1148/ryct.240441}},
  doi          = {{10.1148/ryct.240441}},
  volume       = {{7}},
  year         = {{2025}},
}