Effects of early endotoxemia and dextran-induced anaphylaxis on the size-selectivity of the glomerular filtration barrier in rats.
(2009) In American Journal of Physiology: Renal, Fluid and Electrolyte Physiology 296(2). p.242-248- Abstract
- This study was performed to investigate the glomerular permeability alterations responsible for the microalbuminuria occurring in endotoxemia and during anaphylactic shock. In anaesthetized Wistar rats, the left ureter was catheterized for urine collection, while simultaneously, blood access was achieved. Endotoxemia was induced by Lipopolysaccharide (LPS) from E. Coli, and glomerular permeability assessed at 60, 90 (ENDO-(60)/90; n=7) and 120 min (ENDO-120; n=7). Anaphylaxis was induced by a bolus dose of Dextran-70, and glomerular permeability assessed at 5 min (ANA-5; n=8) and 40 min (ANA-40; n=9). Sham animals, were followed for either 5 or 120 min. The glomerular sieving coefficients () to FITC-Ficoll (70/400) were determined from... (More)
- This study was performed to investigate the glomerular permeability alterations responsible for the microalbuminuria occurring in endotoxemia and during anaphylactic shock. In anaesthetized Wistar rats, the left ureter was catheterized for urine collection, while simultaneously, blood access was achieved. Endotoxemia was induced by Lipopolysaccharide (LPS) from E. Coli, and glomerular permeability assessed at 60, 90 (ENDO-(60)/90; n=7) and 120 min (ENDO-120; n=7). Anaphylaxis was induced by a bolus dose of Dextran-70, and glomerular permeability assessed at 5 min (ANA-5; n=8) and 40 min (ANA-40; n=9). Sham animals, were followed for either 5 or 120 min. The glomerular sieving coefficients () to FITC-Ficoll (70/400) were determined from plasma and urine samples and assessed using size-exclusion chromatography (HPLC). 2 h after start of the LPS infusion, but not at 60 or 90 min, for Ficoll70A had increased markedly (from 2.91 x 10(-5) +/- 6.33 x 10(-6) to 7.78 x 10(-5) +/- 6.21 x 10(-6) (P<0.001)). In anaphylaxis there was a large increase in for Ficolls >60 A in mol. radius already at 5 min, but the glomerular permeability was completely restored at 40 min. In conclusion, there was a transient, immediate increment of glomerular permeability in dextran-induced anaphylaxis, which was completely reversible within 40 min. By contrast, endotoxemia caused an increase in glomerular permeability that was manifest first after 2 h. In both cases to large Ficoll molecules were markedly increased, reflecting an increase in the number of large pores in the glomerular filter. Key words: capillary permeability, Ficoll, sieving coefficient, albumin. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1271536
- author
- Axelsson, Josefin LU ; Rippe, Anna LU ; Venturoli, Daniele LU ; Sward, Per and Rippe, Bengt LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- systemic inflammatory response syndrome, endotoxin
- in
- American Journal of Physiology: Renal, Fluid and Electrolyte Physiology
- volume
- 296
- issue
- 2
- pages
- 242 - 248
- publisher
- American Physiological Society
- external identifiers
-
- wos:000262782700003
- pmid:19004933
- scopus:60549093839
- ISSN
- 0363-6127
- DOI
- 10.1152/ajprenal.90263.2008
- language
- English
- LU publication?
- yes
- id
- f4905807-ca09-4797-a704-9438a5aebd3f (old id 1271536)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/19004933?dopt=Abstract
- date added to LUP
- 2016-04-01 12:08:54
- date last changed
- 2022-03-21 00:12:00
@article{f4905807-ca09-4797-a704-9438a5aebd3f, abstract = {{This study was performed to investigate the glomerular permeability alterations responsible for the microalbuminuria occurring in endotoxemia and during anaphylactic shock. In anaesthetized Wistar rats, the left ureter was catheterized for urine collection, while simultaneously, blood access was achieved. Endotoxemia was induced by Lipopolysaccharide (LPS) from E. Coli, and glomerular permeability assessed at 60, 90 (ENDO-(60)/90; n=7) and 120 min (ENDO-120; n=7). Anaphylaxis was induced by a bolus dose of Dextran-70, and glomerular permeability assessed at 5 min (ANA-5; n=8) and 40 min (ANA-40; n=9). Sham animals, were followed for either 5 or 120 min. The glomerular sieving coefficients () to FITC-Ficoll (70/400) were determined from plasma and urine samples and assessed using size-exclusion chromatography (HPLC). 2 h after start of the LPS infusion, but not at 60 or 90 min, for Ficoll70A had increased markedly (from 2.91 x 10(-5) +/- 6.33 x 10(-6) to 7.78 x 10(-5) +/- 6.21 x 10(-6) (P<0.001)). In anaphylaxis there was a large increase in for Ficolls >60 A in mol. radius already at 5 min, but the glomerular permeability was completely restored at 40 min. In conclusion, there was a transient, immediate increment of glomerular permeability in dextran-induced anaphylaxis, which was completely reversible within 40 min. By contrast, endotoxemia caused an increase in glomerular permeability that was manifest first after 2 h. In both cases to large Ficoll molecules were markedly increased, reflecting an increase in the number of large pores in the glomerular filter. Key words: capillary permeability, Ficoll, sieving coefficient, albumin.}}, author = {{Axelsson, Josefin and Rippe, Anna and Venturoli, Daniele and Sward, Per and Rippe, Bengt}}, issn = {{0363-6127}}, keywords = {{systemic inflammatory response syndrome; endotoxin}}, language = {{eng}}, number = {{2}}, pages = {{242--248}}, publisher = {{American Physiological Society}}, series = {{American Journal of Physiology: Renal, Fluid and Electrolyte Physiology}}, title = {{Effects of early endotoxemia and dextran-induced anaphylaxis on the size-selectivity of the glomerular filtration barrier in rats.}}, url = {{http://dx.doi.org/10.1152/ajprenal.90263.2008}}, doi = {{10.1152/ajprenal.90263.2008}}, volume = {{296}}, year = {{2009}}, }