Rapid fragmentation of the endoplasmic reticulum in cortical neurons of the mouse brain in situ following cardiac arrest.
(2011) In Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 31. p.1663-1667- Abstract
- Neuronal endoplasmic reticulum (ER), continuous from soma to dendritic spines, undergoes rapid fragmentation in response to N-methyl-D-aspartate (NMDA) receptor stimulation in hippocampal slices and neuronal primary cultures. Here, we show that ER fragments in the mouse brain following cardiac arrest (CA) induced brain ischemia. The ER structure was assessed in vivo in cortical pyramidal neurons in transgenic mice expressing ER-targeted GFP using two-photon laser scanning microscopy with fluorescence recovery after photobleaching (FRAP). Endoplasmic reticulum fragmentation occurred 1 to 2 minutes after CA and once induced, fragmentation was rapid (<15 seconds). We propose that acute ER fragmentation may be a protective response against... (More)
- Neuronal endoplasmic reticulum (ER), continuous from soma to dendritic spines, undergoes rapid fragmentation in response to N-methyl-D-aspartate (NMDA) receptor stimulation in hippocampal slices and neuronal primary cultures. Here, we show that ER fragments in the mouse brain following cardiac arrest (CA) induced brain ischemia. The ER structure was assessed in vivo in cortical pyramidal neurons in transgenic mice expressing ER-targeted GFP using two-photon laser scanning microscopy with fluorescence recovery after photobleaching (FRAP). Endoplasmic reticulum fragmentation occurred 1 to 2 minutes after CA and once induced, fragmentation was rapid (<15 seconds). We propose that acute ER fragmentation may be a protective response against severe ischemic stress.Journal of Cerebral Blood Flow & Metabolism advance online publication, 6 April 2011; doi:10.1038/jcbfm.2011.37. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1937483
- author
- Kucharz, Krzysztof LU ; Wieloch, Tadeusz LU and Toresson, Håkan LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
- volume
- 31
- pages
- 1663 - 1667
- publisher
- Nature Publishing Group
- external identifiers
-
- wos:000293342100002
- pmid:21468089
- scopus:79960982266
- pmid:21468089
- ISSN
- 1559-7016
- DOI
- 10.1038/jcbfm.2011.37
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Clinical Memory Research Unit (013242610), Laboratory for Experimental Brain Research (013041000)
- id
- f491c76d-e6d5-4aa0-9c31-778aa09e4c7e (old id 1937483)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21468089?dopt=Abstract
- date added to LUP
- 2016-04-01 15:02:10
- date last changed
- 2022-03-22 03:09:05
@article{f491c76d-e6d5-4aa0-9c31-778aa09e4c7e, abstract = {{Neuronal endoplasmic reticulum (ER), continuous from soma to dendritic spines, undergoes rapid fragmentation in response to N-methyl-D-aspartate (NMDA) receptor stimulation in hippocampal slices and neuronal primary cultures. Here, we show that ER fragments in the mouse brain following cardiac arrest (CA) induced brain ischemia. The ER structure was assessed in vivo in cortical pyramidal neurons in transgenic mice expressing ER-targeted GFP using two-photon laser scanning microscopy with fluorescence recovery after photobleaching (FRAP). Endoplasmic reticulum fragmentation occurred 1 to 2 minutes after CA and once induced, fragmentation was rapid (<15 seconds). We propose that acute ER fragmentation may be a protective response against severe ischemic stress.Journal of Cerebral Blood Flow & Metabolism advance online publication, 6 April 2011; doi:10.1038/jcbfm.2011.37.}}, author = {{Kucharz, Krzysztof and Wieloch, Tadeusz and Toresson, Håkan}}, issn = {{1559-7016}}, language = {{eng}}, pages = {{1663--1667}}, publisher = {{Nature Publishing Group}}, series = {{Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism}}, title = {{Rapid fragmentation of the endoplasmic reticulum in cortical neurons of the mouse brain in situ following cardiac arrest.}}, url = {{https://lup.lub.lu.se/search/files/4308012/1971416.pdf}}, doi = {{10.1038/jcbfm.2011.37}}, volume = {{31}}, year = {{2011}}, }