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Carbonic anhydrases in human keratinocytes and their regulation by all-trans retinoic acid and 1α,25-dihydroxyvitamin D3

Suri, Bani Kaur; Schmidtchen, Artur LU and Verma, Navin Kumar (2019) In Experimental Dermatology
Abstract

Carbonic anhydrases (CAs) are ubiquitously expressed enzymes and catalyse an important physiological reaction of interconverting the hydration of carbon dioxide to bicarbonates, which is crucial for maintaining acid/base equilibrium in certain tissues. While 15 different isoforms of CAs are present in various cell types in human tissues, their expression pattern in the epidermis remains to be investigated. Here, we report the expression of 5 CA isoforms (CAII, CAIX, CAXI, CAXII and CAXIII) in human primary keratinocytes. Further, we demonstrate that the expression of CAII and CAIX in these cells is significantly up-regulated by the biologically active metabolites of vitamin A (all-trans retinoic acid) and vitamin D... (More)

Carbonic anhydrases (CAs) are ubiquitously expressed enzymes and catalyse an important physiological reaction of interconverting the hydration of carbon dioxide to bicarbonates, which is crucial for maintaining acid/base equilibrium in certain tissues. While 15 different isoforms of CAs are present in various cell types in human tissues, their expression pattern in the epidermis remains to be investigated. Here, we report the expression of 5 CA isoforms (CAII, CAIX, CAXI, CAXII and CAXIII) in human primary keratinocytes. Further, we demonstrate that the expression of CAII and CAIX in these cells is significantly up-regulated by the biologically active metabolites of vitamin A (all-trans retinoic acid) and vitamin D (1α,25-dihydroxyvitamin D3), respectively. Taken together, apart from providing new information on the expression of CAs in the skin, our results highlight a previously undisclosed connection between vitamin A and CAII expression and vitamin D and CAIX expression.

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author
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
carbonic anhydrases, retinoic acid, vitamin A, vitamin D
in
Experimental Dermatology
publisher
Wiley-Blackwell
external identifiers
  • scopus:85068509425
ISSN
0906-6705
DOI
10.1111/exd.13976
language
English
LU publication?
yes
id
f4a821a3-b900-4c65-a6f9-7ca990479e76
date added to LUP
2019-07-19 10:59:05
date last changed
2019-08-14 04:41:59
@article{f4a821a3-b900-4c65-a6f9-7ca990479e76,
  abstract     = {<p>Carbonic anhydrases (CAs) are ubiquitously expressed enzymes and catalyse an important physiological reaction of interconverting the hydration of carbon dioxide to bicarbonates, which is crucial for maintaining acid/base equilibrium in certain tissues. While 15 different isoforms of CAs are present in various cell types in human tissues, their expression pattern in the epidermis remains to be investigated. Here, we report the expression of 5 CA isoforms (CAII, CAIX, CAXI, CAXII and CAXIII) in human primary keratinocytes. Further, we demonstrate that the expression of CAII and CAIX in these cells is significantly up-regulated by the biologically active metabolites of vitamin A (all-trans retinoic acid) and vitamin D (1α,25-dihydroxyvitamin D<sub>3</sub>), respectively. Taken together, apart from providing new information on the expression of CAs in the skin, our results highlight a previously undisclosed connection between vitamin A and CAII expression and vitamin D and CAIX expression.</p>},
  author       = {Suri, Bani Kaur and Schmidtchen, Artur and Verma, Navin Kumar},
  issn         = {0906-6705},
  keyword      = {carbonic anhydrases,retinoic acid,vitamin A,vitamin D},
  language     = {eng},
  month        = {05},
  publisher    = {Wiley-Blackwell},
  series       = {Experimental Dermatology},
  title        = {Carbonic anhydrases in human keratinocytes and their regulation by all-trans retinoic acid and 1α,25-dihydroxyvitamin D<sub>3</sub>},
  url          = {http://dx.doi.org/10.1111/exd.13976},
  year         = {2019},
}