Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Molecular characterization of a galactomannan extracted from Tara (Caesalpinia spinosa) seeds

Ibieta, Gabriela LU ; Bustos, Atma-Sol ; Ortiz-Sempértegui, Jimena LU ; Linares-Pastén, Javier A. LU orcid and Peñarrieta, J. Mauricio (2023) In Scientific Reports 13.
Abstract
Tara gum (TG) is a polysaccharide extracted from the seeds of a South American tree called Tara (Caesalpinia spinosa). TG is a galactomannan with many applications in the food industry, mainly as an emulsifier and stabilizer agent. In addition, it is also used in the paper and cosmetic industries. In the present study, we performed a molecular characterization based on chemical composition and physicochemical properties to understand the properties behind TG applications. TG was extracted and purified from Tara seeds distributed in different ecoregions of Bolivia. The monosaccharide composition analysis was determined by high-performance anion-exchange chromatography/pulsed amperometric detection (HPAEC-PAD). At the same time, their... (More)
Tara gum (TG) is a polysaccharide extracted from the seeds of a South American tree called Tara (Caesalpinia spinosa). TG is a galactomannan with many applications in the food industry, mainly as an emulsifier and stabilizer agent. In addition, it is also used in the paper and cosmetic industries. In the present study, we performed a molecular characterization based on chemical composition and physicochemical properties to understand the properties behind TG applications. TG was extracted and purified from Tara seeds distributed in different ecoregions of Bolivia. The monosaccharide composition analysis was determined by high-performance anion-exchange chromatography/pulsed amperometric detection (HPAEC-PAD). At the same time, their molecular characteristics, such as molar mass, root-mean-square radius, hydrodynamic radius, conformation, and densities, were studied by asymmetrical flow field-flow fractionation coupled to multi-angle light scattering refractive index (AF4-MALS-dRI), also the specific refractive index increment (dn/dc) was determined for the first time using AF4 for TG. The results revealed that the gum samples are galactomannans composed of mannose (Man) and galactose (Gal) in a ratio of 3.37 (Man/Gal), with an average molar mass range from 2.460 × 107 to 3.699 × 107 Da, distributed in a single population. The root-mean-square radius range from 260.4 to 281.6 nm, and dn/dc is 0.1454. The Kratky plots based on 14 scattering angles indicated that the conformation of all samples corresponds to random coil monodisperse, while their gyration radius/hydrodynamic radius ratio (ρ) is high. All these results suggest that the chains have a low branched density, consistent with the Gal/Man composition. To the best of our knowledge, we report for the first time an integrated physicochemical study of TG relevant to developing emulsifier and stabilizer formulations. (Less)
Please use this url to cite or link to this publication:
author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Scientific Reports
volume
13
article number
21893
pages
11 pages
publisher
Nature Publishing Group
external identifiers
  • scopus:85179324183
  • pmid:38081901
ISSN
2045-2322
DOI
10.1038/s41598-023-49149-3
language
English
LU publication?
yes
id
f5290451-9d11-4edb-96c4-edbdbd65b094
date added to LUP
2023-12-11 11:37:23
date last changed
2024-03-12 03:00:21
@article{f5290451-9d11-4edb-96c4-edbdbd65b094,
  abstract     = {{Tara gum (TG) is a polysaccharide extracted from the seeds of a South American tree called Tara (<i>Caesalpinia spinosa</i>). TG is a galactomannan with many applications in the food industry, mainly as an emulsifier and stabilizer agent. In addition, it is also used in the paper and cosmetic industries. In the present study, we performed a molecular characterization based on chemical composition and physicochemical properties to understand the properties behind TG applications. TG was extracted and purified from Tara seeds distributed in different ecoregions of Bolivia. The monosaccharide composition analysis was determined by high-performance anion-exchange chromatography/pulsed amperometric detection (HPAEC-PAD). At the same time, their molecular characteristics, such as molar mass, root-mean-square radius, hydrodynamic radius, conformation, and densities, were studied by asymmetrical flow field-flow fractionation coupled to multi-angle light scattering refractive index (AF4-MALS-dRI), also the specific refractive index increment (dn/dc) was determined for the first time using AF4 for TG. The results revealed that the gum samples are galactomannans composed of mannose (Man) and galactose (Gal) in a ratio of 3.37 (Man/Gal), with an average molar mass range from 2.460 × 10<sup>7</sup> to 3.699 × 10<sup>7</sup> Da, distributed in a single population. The root-mean-square radius range from 260.4 to 281.6 nm, and dn/dc is 0.1454. The Kratky plots based on 14 scattering angles indicated that the conformation of all samples corresponds to random coil monodisperse, while their gyration radius/hydrodynamic radius ratio (ρ) is high. All these results suggest that the chains have a low branched density, consistent with the Gal/Man composition. To the best of our knowledge, we report for the first time an integrated physicochemical study of TG relevant to developing emulsifier and stabilizer formulations.}},
  author       = {{Ibieta, Gabriela and Bustos, Atma-Sol and Ortiz-Sempértegui, Jimena and Linares-Pastén, Javier A. and Peñarrieta, J. Mauricio}},
  issn         = {{2045-2322}},
  language     = {{eng}},
  month        = {{12}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Scientific Reports}},
  title        = {{Molecular characterization of a galactomannan extracted from Tara (<i>Caesalpinia spinosa</i>) seeds}},
  url          = {{http://dx.doi.org/10.1038/s41598-023-49149-3}},
  doi          = {{10.1038/s41598-023-49149-3}},
  volume       = {{13}},
  year         = {{2023}},
}