Advanced

Mapping Dynamics of Bacterial Communities in a Full-Scale Drinking Water Distribution System Using Flow Cytometry

Schleich, Caroline ; Chan, Sandy LU ; Pullerits, Kristjan LU ; Besmer, Michael ; Paul, Catherine J. LU ; Rådström, Peter LU and Keucken, Alexander LU (2019) In Water (Switzerland) 11.
Abstract
Microbial monitoring of drinking water is required to guarantee high quality water and to mitigate health hazards. Flow cytometry (FCM) is a fast and robust method that determines bacterial concentrations in liquids. In this study, FCM was applied to monitor the dynamics of the bacterial communities over one year in a full-scale drinking water distribution system (DWDS), following implementation of ultrafiltration (UF) combined with coagulation at the drinking water treatment plant (DWTP). Correlations between the environmental conditions in the DWDS and microbial regrowth were observed, including increases in total cell counts with increasing retention time (correlation coefficient R = 0.89) and increasing water temperature (up to... (More)
Microbial monitoring of drinking water is required to guarantee high quality water and to mitigate health hazards. Flow cytometry (FCM) is a fast and robust method that determines bacterial concentrations in liquids. In this study, FCM was applied to monitor the dynamics of the bacterial communities over one year in a full-scale drinking water distribution system (DWDS), following implementation of ultrafiltration (UF) combined with coagulation at the drinking water treatment plant (DWTP). Correlations between the environmental conditions in the DWDS and microbial regrowth were observed, including increases in total cell counts with increasing retention time (correlation coefficient R = 0.89) and increasing water temperature (up to 5.24-fold increase in cell counts during summer). Temporal and spatial biofilm dynamics affecting the water within the DWDS were also observed, such as changes in the percentage of high nucleic acid bacteria with increasing retention time (correlation coefficient R = −0.79). FCM baselines were defined for specific areas in the DWDS to support future management strategies in this DWDS, including a gradual reduction of chloramine. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Water (Switzerland)
volume
11
pages
14 pages
publisher
MDPI AG
ISSN
2073-4441
DOI
10.3390/w11102137
language
English
LU publication?
yes
id
f71c80d0-c4ba-4e5c-9d9d-93efa4e2fcf5
date added to LUP
2019-10-20 21:50:58
date last changed
2019-11-08 13:45:45
@article{f71c80d0-c4ba-4e5c-9d9d-93efa4e2fcf5,
  abstract     = {Microbial monitoring of drinking water is required to guarantee high quality water and to mitigate health hazards. Flow cytometry (FCM) is a fast and robust method that determines bacterial concentrations in liquids. In this study, FCM was applied to monitor the dynamics of the bacterial communities over one year in a full-scale drinking water distribution system (DWDS), following implementation of ultrafiltration (UF) combined with coagulation at the drinking water treatment plant (DWTP). Correlations between the environmental conditions in the DWDS and microbial regrowth were observed, including increases in total cell counts with increasing retention time (correlation coefficient R = 0.89) and increasing water temperature (up to 5.24-fold increase in cell counts during summer). Temporal and spatial biofilm dynamics affecting the water within the DWDS were also observed, such as changes in the percentage of high nucleic acid bacteria with increasing retention time (correlation coefficient R = −0.79). FCM baselines were defined for specific areas in the DWDS to support future management strategies in this DWDS, including a gradual reduction of chloramine.},
  author       = {Schleich, Caroline  and Chan, Sandy and Pullerits, Kristjan and Besmer, Michael  and Paul, Catherine J.  and Rådström, Peter and Keucken, Alexander},
  issn         = {2073-4441},
  language     = {eng},
  month        = {10},
  publisher    = {MDPI AG},
  series       = {Water (Switzerland)},
  title        = {Mapping Dynamics of Bacterial Communities in a Full-Scale Drinking Water Distribution System Using Flow Cytometry},
  url          = {http://dx.doi.org/10.3390/w11102137},
  doi          = {10.3390/w11102137},
  volume       = {11},
  year         = {2019},
}