A protein kinase Cbeta inhibitor attenuates multidrug resistance of neuroblastoma cells.
(2003) In BMC Cancer 3(1). p.10-10- Abstract
- BACKGROUND: The acquisition of drug resistance is a major reason for poor outcome of neuroblastoma. Protein kinase C (PKC) has been suggested to influence drug resistance in cancer cells. The aim of this study was to elucidate whether inhibition of PKCbeta isoforms influences drug-resistance of neuroblastoma cells. METHODS: The effect of the PKCbeta inhibitor LY379196 on the growth-suppressing effects of different chemotherapeutics on neuroblastoma cells was analyzed with MTT assays. The effect of LY379196 on the accumulation of [3H]vincristine was also investigated RESULTS: The PKCbeta inhibitor LY379196 suppressed the growth of three neuroblastoma cell lines. LY379196 also augmented the growth-suppressive effect of doxorubicin,... (More)
- BACKGROUND: The acquisition of drug resistance is a major reason for poor outcome of neuroblastoma. Protein kinase C (PKC) has been suggested to influence drug resistance in cancer cells. The aim of this study was to elucidate whether inhibition of PKCbeta isoforms influences drug-resistance of neuroblastoma cells. METHODS: The effect of the PKCbeta inhibitor LY379196 on the growth-suppressing effects of different chemotherapeutics on neuroblastoma cells was analyzed with MTT assays. The effect of LY379196 on the accumulation of [3H]vincristine was also investigated RESULTS: The PKCbeta inhibitor LY379196 suppressed the growth of three neuroblastoma cell lines. LY379196 also augmented the growth-suppressive effect of doxorubicin, etoposide, paclitaxel, and vincristine, but not of carboplatin. The effect was most marked for vincristine and for the cell-line (SK-N-BE(2)) that was least sensitive to vincristine. No effect was observed on the non-resistant IMR-32 cells. Two other PKC inhibitors, Go6976 and GF109203X, also enhanced the vincristine effect. The PKC inhibitors caused an increased accumulation of [3H]vincristine in SK-N-BE(2) cells. CONCLUSIONS: This indicates that inhibition of PKCbeta could attenuate multidrug resistance in neuroblastoma cells by augmenting the levels of natural product anticancer drugs in resistant cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/113372
- author
- Svensson, Karin and Larsson, Christer LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- BMC Cancer
- volume
- 3
- issue
- 1
- pages
- 10 - 10
- publisher
- BioMed Central (BMC)
- external identifiers
-
- wos:000182955000001
- pmid:12697075
- scopus:3042834127
- ISSN
- 1471-2407
- DOI
- 10.1186/1471-2407-3-10
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Tumour Cell Biology (013017530)
- id
- f7280d0d-cb92-4c50-ba24-36339cbe7301 (old id 113372)
- alternative location
- http://www.biomedcentral.com/content/pdf/1471-2407-3-10.pdf
- date added to LUP
- 2016-04-01 16:49:31
- date last changed
- 2022-01-28 22:23:15
@article{f7280d0d-cb92-4c50-ba24-36339cbe7301, abstract = {{BACKGROUND: The acquisition of drug resistance is a major reason for poor outcome of neuroblastoma. Protein kinase C (PKC) has been suggested to influence drug resistance in cancer cells. The aim of this study was to elucidate whether inhibition of PKCbeta isoforms influences drug-resistance of neuroblastoma cells. METHODS: The effect of the PKCbeta inhibitor LY379196 on the growth-suppressing effects of different chemotherapeutics on neuroblastoma cells was analyzed with MTT assays. The effect of LY379196 on the accumulation of [3H]vincristine was also investigated RESULTS: The PKCbeta inhibitor LY379196 suppressed the growth of three neuroblastoma cell lines. LY379196 also augmented the growth-suppressive effect of doxorubicin, etoposide, paclitaxel, and vincristine, but not of carboplatin. The effect was most marked for vincristine and for the cell-line (SK-N-BE(2)) that was least sensitive to vincristine. No effect was observed on the non-resistant IMR-32 cells. Two other PKC inhibitors, Go6976 and GF109203X, also enhanced the vincristine effect. The PKC inhibitors caused an increased accumulation of [3H]vincristine in SK-N-BE(2) cells. CONCLUSIONS: This indicates that inhibition of PKCbeta could attenuate multidrug resistance in neuroblastoma cells by augmenting the levels of natural product anticancer drugs in resistant cells.}}, author = {{Svensson, Karin and Larsson, Christer}}, issn = {{1471-2407}}, language = {{eng}}, number = {{1}}, pages = {{10--10}}, publisher = {{BioMed Central (BMC)}}, series = {{BMC Cancer}}, title = {{A protein kinase Cbeta inhibitor attenuates multidrug resistance of neuroblastoma cells.}}, url = {{https://lup.lub.lu.se/search/files/4791616/623743.pdf}}, doi = {{10.1186/1471-2407-3-10}}, volume = {{3}}, year = {{2003}}, }