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A PKCbeta isoform mediates phorbol ester-induced activation of Erk1/2 and expression of neuronal differentiation genes in neuroblastoma cells

Trollér, Ulrika LU ; Zeidman, Ruth LU ; Svensson, Karin and Larsson, Christer LU (2001) In FEBS Letters 508(1). p.126-130
Abstract
Protein kinase C (PKC) activation induces neuronal differentiation of SH-SY5Y neuroblastoma cells. This study examines the role of PKCbeta isoforms in this process. The PKCbeta-specific inhibitor LY379196 had no effect on 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced neurite outgrowth from SH-SY5Y neuroblastoma cells. On the other hand, PKCbeta inhibition suppressed the TPA-stimulated increase in neuropeptide Y mRNA, activation of neuropeptide Y gene promoter elements, and phosphorylation of Erk1/2. The TPA-induced increase in neuropeptide Y expression was also inhibited by the MEK inhibitor PD98059. These data indicate that activation of a PKCbeta isoform, through a pathway involving Erk1/2, leads to increased expression of neuronal... (More)
Protein kinase C (PKC) activation induces neuronal differentiation of SH-SY5Y neuroblastoma cells. This study examines the role of PKCbeta isoforms in this process. The PKCbeta-specific inhibitor LY379196 had no effect on 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced neurite outgrowth from SH-SY5Y neuroblastoma cells. On the other hand, PKCbeta inhibition suppressed the TPA-stimulated increase in neuropeptide Y mRNA, activation of neuropeptide Y gene promoter elements, and phosphorylation of Erk1/2. The TPA-induced increase in neuropeptide Y expression was also inhibited by the MEK inhibitor PD98059. These data indicate that activation of a PKCbeta isoform, through a pathway involving Erk1/2, leads to increased expression of neuronal differentiation genes in neuroblastoma cells. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Growth-associated protein 43, Mitogen-activated protein kinase, Neuroblastoma cell, Neuronal differentiation, Neuropeptide Y, Protein kinase C
in
FEBS Letters
volume
508
issue
1
pages
126 - 130
publisher
Wiley-Blackwell
external identifiers
  • pmid:11707282
  • scopus:0035834240
ISSN
1873-3468
DOI
10.1016/S0014-5793(01)03043-5
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Tumour Cell Biology (013017530), Molecular Medicine (013031200), Division of Molecular Medicine and Gene Therapy (013022010)
id
f7d7255a-be38-4748-a8ed-3051a1441985 (old id 1119765)
date added to LUP
2016-04-01 15:26:33
date last changed
2022-01-28 05:21:40
@article{f7d7255a-be38-4748-a8ed-3051a1441985,
  abstract     = {{Protein kinase C (PKC) activation induces neuronal differentiation of SH-SY5Y neuroblastoma cells. This study examines the role of PKCbeta isoforms in this process. The PKCbeta-specific inhibitor LY379196 had no effect on 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced neurite outgrowth from SH-SY5Y neuroblastoma cells. On the other hand, PKCbeta inhibition suppressed the TPA-stimulated increase in neuropeptide Y mRNA, activation of neuropeptide Y gene promoter elements, and phosphorylation of Erk1/2. The TPA-induced increase in neuropeptide Y expression was also inhibited by the MEK inhibitor PD98059. These data indicate that activation of a PKCbeta isoform, through a pathway involving Erk1/2, leads to increased expression of neuronal differentiation genes in neuroblastoma cells.}},
  author       = {{Trollér, Ulrika and Zeidman, Ruth and Svensson, Karin and Larsson, Christer}},
  issn         = {{1873-3468}},
  keywords     = {{Growth-associated protein 43; Mitogen-activated protein kinase; Neuroblastoma cell; Neuronal differentiation; Neuropeptide Y; Protein kinase C}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{126--130}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{FEBS Letters}},
  title        = {{A PKCbeta isoform mediates phorbol ester-induced activation of Erk1/2 and expression of neuronal differentiation genes in neuroblastoma cells}},
  url          = {{http://dx.doi.org/10.1016/S0014-5793(01)03043-5}},
  doi          = {{10.1016/S0014-5793(01)03043-5}},
  volume       = {{508}},
  year         = {{2001}},
}