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Safe lung preservation for twenty-four hours with Perfadex

Steen, Stig LU ; Kimblad, Per Ola LU ; Sjöberg, Trygve LU ; Lindberg, L LU ; Ingemansson, Richard LU and Massa, G (1994) In Annals of Thoracic Surgery 57(2). p.450-457
Abstract
The function of six porcine left lung allografts was studied after pulmonary (140 mL/kg) and bronchial (50 mL/kg) artery perfusion with Perfadex (Kabi Pharmacia, Uppsala, Sweden) at room temperature, followed by 24-hour storage of the lungs in an atelectatic state in 6 degrees to 8 degrees C Perfadex, which is a low-potassium-dextran solution. Left lung transplantation was done followed by right pneumonectomy, thereby making all the animals 100% dependent for their survival on the transplanted lungs. The pigs (mean weight = 56 kg, range = 51 to 58 kg, n = 18; 6 donors, 6 recipients, and 6 sham operated) were ventilated with a volume-controlled ventilator (20 breaths/min, 500 mL tidal volume, 8 cm H2O positive end-expiratory pressure,... (More)
The function of six porcine left lung allografts was studied after pulmonary (140 mL/kg) and bronchial (50 mL/kg) artery perfusion with Perfadex (Kabi Pharmacia, Uppsala, Sweden) at room temperature, followed by 24-hour storage of the lungs in an atelectatic state in 6 degrees to 8 degrees C Perfadex, which is a low-potassium-dextran solution. Left lung transplantation was done followed by right pneumonectomy, thereby making all the animals 100% dependent for their survival on the transplanted lungs. The pigs (mean weight = 56 kg, range = 51 to 58 kg, n = 18; 6 donors, 6 recipients, and 6 sham operated) were ventilated with a volume-controlled ventilator (20 breaths/min, 500 mL tidal volume, 8 cm H2O positive end-expiratory pressure, inspired oxygen fraction = 0.5). All the transplanted animals were in good condition throughout the 24-hour observation period with arterial oxygen tensions around 25 kPa (188 mm Hg) and arterial carbon dioxide tensions around 5 kPa (38 mm Hg). The mean pulmonary arterial pressure was around 30 mm Hg, and the pulmonary vascular resistance around 500 dyn.s.cm-5; neither showed any tendency to change with time. After 24 hours the inspired oxygen fraction was increased to 1.0 and the arterial oxygen tension increased to 43.3 +/- 5 kPa (325 +/- 38 mm Hg) (mean +/- standard error of the mean; n = 6). A sham operation (bilateral thoracotomy, right pneumonectomy) was done in 6 pigs, which were followed up for 24 hours.(ABSTRACT TRUNCATED AT 250 WORDS) (Less)
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publishing date
type
Contribution to journal
publication status
published
subject
in
Annals of Thoracic Surgery
volume
57
issue
2
pages
8 pages
publisher
Elsevier
external identifiers
  • pmid:8311611
  • scopus:0028346742
  • pmid:8311611
ISSN
1552-6259
DOI
10.1016/0003-4975(94)91016-2
language
English
LU publication?
no
id
f7efd7aa-2a2b-4304-9caa-c8469d68dbe2 (old id 1108581)
date added to LUP
2016-04-01 12:34:55
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2021-09-19 03:15:18
@article{f7efd7aa-2a2b-4304-9caa-c8469d68dbe2,
  abstract     = {{The function of six porcine left lung allografts was studied after pulmonary (140 mL/kg) and bronchial (50 mL/kg) artery perfusion with Perfadex (Kabi Pharmacia, Uppsala, Sweden) at room temperature, followed by 24-hour storage of the lungs in an atelectatic state in 6 degrees to 8 degrees C Perfadex, which is a low-potassium-dextran solution. Left lung transplantation was done followed by right pneumonectomy, thereby making all the animals 100% dependent for their survival on the transplanted lungs. The pigs (mean weight = 56 kg, range = 51 to 58 kg, n = 18; 6 donors, 6 recipients, and 6 sham operated) were ventilated with a volume-controlled ventilator (20 breaths/min, 500 mL tidal volume, 8 cm H2O positive end-expiratory pressure, inspired oxygen fraction = 0.5). All the transplanted animals were in good condition throughout the 24-hour observation period with arterial oxygen tensions around 25 kPa (188 mm Hg) and arterial carbon dioxide tensions around 5 kPa (38 mm Hg). The mean pulmonary arterial pressure was around 30 mm Hg, and the pulmonary vascular resistance around 500 dyn.s.cm-5; neither showed any tendency to change with time. After 24 hours the inspired oxygen fraction was increased to 1.0 and the arterial oxygen tension increased to 43.3 +/- 5 kPa (325 +/- 38 mm Hg) (mean +/- standard error of the mean; n = 6). A sham operation (bilateral thoracotomy, right pneumonectomy) was done in 6 pigs, which were followed up for 24 hours.(ABSTRACT TRUNCATED AT 250 WORDS)}},
  author       = {{Steen, Stig and Kimblad, Per Ola and Sjöberg, Trygve and Lindberg, L and Ingemansson, Richard and Massa, G}},
  issn         = {{1552-6259}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{450--457}},
  publisher    = {{Elsevier}},
  series       = {{Annals of Thoracic Surgery}},
  title        = {{Safe lung preservation for twenty-four hours with Perfadex}},
  url          = {{http://dx.doi.org/10.1016/0003-4975(94)91016-2}},
  doi          = {{10.1016/0003-4975(94)91016-2}},
  volume       = {{57}},
  year         = {{1994}},
}