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Rapid confocal imaging of vesicle-to-sponge phase droplet transition in dilute dispersions of the C 10 E 3 surfactant

Schroder, André Pierre ; Crassous, Jérôme Joseph LU ; Marques, Carlos Manuel and Olsson, Ulf LU (2019) In Scientific Reports 9(1).
Abstract


The lamellar-to-sponge phase transition of fluorescently labelled large unilamellar vesicles (LUVs) of the non-ionic surfactant triethylene glycol mono n-decyl ether (C
10
E
3
) was investigated in situ by confocal laser scanning microscopy (CLSM). Stable dispersions of micrometer-sized C
10
... (More)


The lamellar-to-sponge phase transition of fluorescently labelled large unilamellar vesicles (LUVs) of the non-ionic surfactant triethylene glycol mono n-decyl ether (C
10
E
3
) was investigated in situ by confocal laser scanning microscopy (CLSM). Stable dispersions of micrometer-sized C
10
E
3
LUVs were prepared at 20 °C and quickly heated at different temperatures close to the lamellar-to-sponge phase transition temperature. Phase transition of the strongly fluctuating individual vesicles into micrometre-sized sponge phase droplets was observed to occur via manyfold multilamellar morphologies with increasing membrane confinement through inter- and intra- lamellar fusion. The very low bending rigidity and lateral tension of the C
10
E
3
bilayer were supported by quantitative image analysis of a stable fluctuating membrane using both flicker noise spectroscopy and spatial autocorrelation function.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Scientific Reports
volume
9
issue
1
article number
2292
publisher
Nature Publishing Group
external identifiers
  • scopus:85061852101
  • pmid:30783162
ISSN
2045-2322
DOI
10.1038/s41598-019-38620-9
language
English
LU publication?
yes
id
f838e7d8-ed4c-4b5e-abf0-a793606f8d9f
date added to LUP
2019-03-01 08:59:35
date last changed
2020-01-13 01:30:56
@article{f838e7d8-ed4c-4b5e-abf0-a793606f8d9f,
  abstract     = {<p><br>
                                                         The lamellar-to-sponge phase transition of fluorescently labelled large unilamellar vesicles (LUVs) of the non-ionic surfactant triethylene glycol mono n-decyl ether (C                             <br>
                            <sub>10</sub><br>
                                                         E                             <br>
                            <sub>3</sub><br>
                                                         ) was investigated in situ by confocal laser scanning microscopy (CLSM). Stable dispersions of micrometer-sized C                             <br>
                            <sub>10</sub><br>
                                                         E                             <br>
                            <sub>3</sub><br>
                                                          LUVs were prepared at 20 °C and quickly heated at different temperatures close to the lamellar-to-sponge phase transition temperature. Phase transition of the strongly fluctuating individual vesicles into micrometre-sized sponge phase droplets was observed to occur via manyfold multilamellar morphologies with increasing membrane confinement through inter- and intra- lamellar fusion. The very low bending rigidity and lateral tension of the C                             <br>
                            <sub>10</sub><br>
                                                         E                             <br>
                            <sub>3</sub><br>
                                                          bilayer were supported by quantitative image analysis of a stable fluctuating membrane using both flicker noise spectroscopy and spatial autocorrelation function.                         <br>
                        </p>},
  author       = {Schroder, André Pierre and Crassous, Jérôme Joseph and Marques, Carlos Manuel and Olsson, Ulf},
  issn         = {2045-2322},
  language     = {eng},
  month        = {02},
  number       = {1},
  publisher    = {Nature Publishing Group},
  series       = {Scientific Reports},
  title        = {Rapid confocal imaging of vesicle-to-sponge phase droplet transition in dilute dispersions of the C                         
                        <sub>10</sub>
                                                 E                         
                        <sub>3</sub>
                                                  surfactant},
  url          = {http://dx.doi.org/10.1038/s41598-019-38620-9},
  doi          = {10.1038/s41598-019-38620-9},
  volume       = {9},
  year         = {2019},
}