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Quantification of egg proteome changes during fertilization in sterlet Acipenser ruthenus

Niksirat, Hamid; Andersson, Liselotte LU ; Golpour, Amin; Chupani, Latifeh and James, Peter LU (2017) In Biochemical and Biophysical Research Communications 490(2). p.189-193
Abstract

Eggs of sterlet are discharged outside into ambient aquatic environment where egg activation and fertilization occur. Effects of different activation media including freshwater and clay suspension on protein abundances of egg were quantified in sterlet Acipenser ruthenus. In-gel digestion and high resolution mass spectrometry were used for label-free protein quantification in the eggs of five females. No significant (p > 0.05) difference was found between protein abundances in eggs activated with different media. However, results showed significant (p < 0.05, fold change ≥2) reduction in the abundances of nine proteins including six glycoproteins, enolase and heat shock protein in activated groups compared to freshly ovulated eggs... (More)

Eggs of sterlet are discharged outside into ambient aquatic environment where egg activation and fertilization occur. Effects of different activation media including freshwater and clay suspension on protein abundances of egg were quantified in sterlet Acipenser ruthenus. In-gel digestion and high resolution mass spectrometry were used for label-free protein quantification in the eggs of five females. No significant (p > 0.05) difference was found between protein abundances in eggs activated with different media. However, results showed significant (p < 0.05, fold change ≥2) reduction in the abundances of nine proteins including six glycoproteins, enolase and heat shock protein in activated groups compared to freshly ovulated eggs as control. The fact that abundance of proteasome subunit alpha significantly reduced only in eggs which were activated by clay suspension suggests that activation medium can somehow intervene with protein regulation during fertilization. In conclusion, external fertilization in sturgeon egg is accompanied by huge release of proteins into the external environment that may participate in the construction of a transient microenvironment around egg for attraction and protection of spermatozoa to ensure ensuing fertilization. Data are available via ProteomeXchange with identifier PXD006232.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Egg, Fertilization, Label-free, Protein, Quantitative
in
Biochemical and Biophysical Research Communications
volume
490
issue
2
pages
189 - 193
publisher
Elsevier
external identifiers
  • scopus:85020693663
  • wos:000406646600018
ISSN
0006-291X
DOI
10.1016/j.bbrc.2017.06.019
language
English
LU publication?
yes
id
f8591961-b3e2-4149-9161-a3c2a030fad8
date added to LUP
2017-07-04 13:03:58
date last changed
2018-01-07 12:10:33
@article{f8591961-b3e2-4149-9161-a3c2a030fad8,
  abstract     = {<p>Eggs of sterlet are discharged outside into ambient aquatic environment where egg activation and fertilization occur. Effects of different activation media including freshwater and clay suspension on protein abundances of egg were quantified in sterlet Acipenser ruthenus. In-gel digestion and high resolution mass spectrometry were used for label-free protein quantification in the eggs of five females. No significant (p &gt; 0.05) difference was found between protein abundances in eggs activated with different media. However, results showed significant (p &lt; 0.05, fold change ≥2) reduction in the abundances of nine proteins including six glycoproteins, enolase and heat shock protein in activated groups compared to freshly ovulated eggs as control. The fact that abundance of proteasome subunit alpha significantly reduced only in eggs which were activated by clay suspension suggests that activation medium can somehow intervene with protein regulation during fertilization. In conclusion, external fertilization in sturgeon egg is accompanied by huge release of proteins into the external environment that may participate in the construction of a transient microenvironment around egg for attraction and protection of spermatozoa to ensure ensuing fertilization. Data are available via ProteomeXchange with identifier PXD006232.</p>},
  author       = {Niksirat, Hamid and Andersson, Liselotte and Golpour, Amin and Chupani, Latifeh and James, Peter},
  issn         = {0006-291X},
  keyword      = {Egg,Fertilization,Label-free,Protein,Quantitative},
  language     = {eng},
  number       = {2},
  pages        = {189--193},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {Quantification of egg proteome changes during fertilization in sterlet Acipenser ruthenus},
  url          = {http://dx.doi.org/10.1016/j.bbrc.2017.06.019},
  volume       = {490},
  year         = {2017},
}