Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Serotonin- and Dopamine-Related Gene Expression in db/db Mice Islets and in MIN6 β -Cells Treated with Palmitate and Oleate

Cataldo, L. R. LU orcid ; Mizgier, M. L. ; Busso, D. ; Olmos, P. ; Galgani, J. E. ; Valenzuela, R. ; Mezzano, D. ; Aranda, E. ; Cortés, V. A. and Santos, J. L. (2016) In Journal of Diabetes Research 2016. p.1-12
Abstract

High circulating nonesterified fatty acids (NEFAs) concentration, often reported in diabetes, leads to impaired glucose-stimulated insulin secretion (GSIS) through not yet well-defined mechanisms. Serotonin and dopamine might contribute to NEFA-dependent β-cell dysfunction, since extracellular signal of these monoamines decreases GSIS. Moreover, palmitate-treated β-cells may enhance the expression of the serotonin receptor Htr2c, affecting insulin secretion. Additionally, the expression of monoamine-oxidase type B (Maob) seems to be lower in islets from humans and mice with diabetes compared to nondiabetic islets, which may lead to increased monoamine concentrations. We assessed the expression of serotonin- and dopamine-related genes in... (More)

High circulating nonesterified fatty acids (NEFAs) concentration, often reported in diabetes, leads to impaired glucose-stimulated insulin secretion (GSIS) through not yet well-defined mechanisms. Serotonin and dopamine might contribute to NEFA-dependent β-cell dysfunction, since extracellular signal of these monoamines decreases GSIS. Moreover, palmitate-treated β-cells may enhance the expression of the serotonin receptor Htr2c, affecting insulin secretion. Additionally, the expression of monoamine-oxidase type B (Maob) seems to be lower in islets from humans and mice with diabetes compared to nondiabetic islets, which may lead to increased monoamine concentrations. We assessed the expression of serotonin- and dopamine-related genes in islets from db/db and wild-type (WT) mice. In addition, the effect of palmitate and oleate on the expression of such genes, 5HT content, and GSIS in MIN6 β-cell was determined. Lower Maob expression was found in islets from db/db versus WT mice and in MIN6 β-cells in response to palmitate and oleate treatment compared to vehicle. Reduced 5HT content and impaired GSIS in response to palmitate (-25%; p < 0.0001) and oleate (-43%; p < 0.0001) were detected in MIN6 β-cells. In conclusion, known defects of GSIS in islets from db/db mice and MIN6 β-cells treated with NEFAs are accompanied by reduced Maob expression and reduced 5HT content.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
in
Journal of Diabetes Research
volume
2016
article number
3793781
pages
1 - 12
publisher
Hindawi Limited
external identifiers
  • pmid:27366756
  • scopus:84976519849
ISSN
2314-6745
DOI
10.1155/2016/3793781
language
English
LU publication?
no
id
f91095ee-ca81-468e-849b-74d973a35423
date added to LUP
2018-06-04 10:39:27
date last changed
2024-08-05 18:30:48
@article{f91095ee-ca81-468e-849b-74d973a35423,
  abstract     = {{<p>High circulating nonesterified fatty acids (NEFAs) concentration, often reported in diabetes, leads to impaired glucose-stimulated insulin secretion (GSIS) through not yet well-defined mechanisms. Serotonin and dopamine might contribute to NEFA-dependent β-cell dysfunction, since extracellular signal of these monoamines decreases GSIS. Moreover, palmitate-treated β-cells may enhance the expression of the serotonin receptor Htr2c, affecting insulin secretion. Additionally, the expression of monoamine-oxidase type B (Maob) seems to be lower in islets from humans and mice with diabetes compared to nondiabetic islets, which may lead to increased monoamine concentrations. We assessed the expression of serotonin- and dopamine-related genes in islets from db/db and wild-type (WT) mice. In addition, the effect of palmitate and oleate on the expression of such genes, 5HT content, and GSIS in MIN6 β-cell was determined. Lower Maob expression was found in islets from db/db versus WT mice and in MIN6 β-cells in response to palmitate and oleate treatment compared to vehicle. Reduced 5HT content and impaired GSIS in response to palmitate (-25%; p &lt; 0.0001) and oleate (-43%; p &lt; 0.0001) were detected in MIN6 β-cells. In conclusion, known defects of GSIS in islets from db/db mice and MIN6 β-cells treated with NEFAs are accompanied by reduced Maob expression and reduced 5HT content.</p>}},
  author       = {{Cataldo, L. R. and Mizgier, M. L. and Busso, D. and Olmos, P. and Galgani, J. E. and Valenzuela, R. and Mezzano, D. and Aranda, E. and Cortés, V. A. and Santos, J. L.}},
  issn         = {{2314-6745}},
  language     = {{eng}},
  month        = {{01}},
  pages        = {{1--12}},
  publisher    = {{Hindawi Limited}},
  series       = {{Journal of Diabetes Research}},
  title        = {{Serotonin- and Dopamine-Related Gene Expression in db/db Mice Islets and in MIN6 β -Cells Treated with Palmitate and Oleate}},
  url          = {{http://dx.doi.org/10.1155/2016/3793781}},
  doi          = {{10.1155/2016/3793781}},
  volume       = {{2016}},
  year         = {{2016}},
}