Advanced

Respiratory chain complex III deficiency due to mutated BCS1L : A novel phenotype with encephalomyopathy, partially phenocopied in a Bcs1l mutant mouse model

Tegelberg, Saara LU ; Tomašić, Nikica; Kallijärvi, Jukka LU ; Purhonen, Janne; Elmér, Eskil LU ; Lindberg, Eva; Nord, David Gisselsson LU ; Soller, Maria LU ; Lesko, Nicole and Wedell, Anna, et al. (2017) In Orphanet Journal of Rare Diseases 12(1).
Abstract

Background: Mitochondrial diseases due to defective respiratory chain complex III (CIII) are relatively uncommon. The assembly of the eleven-subunit CIII is completed by the insertion of the Rieske iron-sulfur protein, a process for which BCS1L protein is indispensable. Mutations in the BCS1L gene constitute the most common diagnosed cause of CIII deficiency, and the phenotypic spectrum arising from mutations in this gene is wide. Results: A case of CIII deficiency was investigated in depth to assess respiratory chain function and assembly, and brain, skeletal muscle and liver histology. Exome sequencing was performed to search for the causative mutation(s). The patient's platelets and muscle mitochondria showed respiration defects and... (More)

Background: Mitochondrial diseases due to defective respiratory chain complex III (CIII) are relatively uncommon. The assembly of the eleven-subunit CIII is completed by the insertion of the Rieske iron-sulfur protein, a process for which BCS1L protein is indispensable. Mutations in the BCS1L gene constitute the most common diagnosed cause of CIII deficiency, and the phenotypic spectrum arising from mutations in this gene is wide. Results: A case of CIII deficiency was investigated in depth to assess respiratory chain function and assembly, and brain, skeletal muscle and liver histology. Exome sequencing was performed to search for the causative mutation(s). The patient's platelets and muscle mitochondria showed respiration defects and defective assembly of CIII was detected in fibroblast mitochondria. The patient was compound heterozygous for two novel mutations in BCS1L, c.306A > T and c.399delA. In the cerebral cortex a specific pattern of astrogliosis and widespread loss of microglia was observed. Further analysis showed loss of Kupffer cells in the liver. These changes were not found in infants suffering from GRACILE syndrome, the most severe BCS1L-related disorder causing early postnatal mortality, but were partially corroborated in a knock-in mouse model of BCS1L deficiency. Conclusions: We describe two novel compound heterozygous mutations in BCS1L causing CIII deficiency. The pathogenicity of one of the mutations was unexpected and points to the importance of combining next generation sequencing with a biochemical approach when investigating these patients. We further show novel manifestations in brain, skeletal muscle and liver, including abnormality in specialized resident macrophages (microglia and Kupffer cells). These novel phenotypes forward our understanding of CIII deficiencies caused by BCS1L mutations.

(Less)
Please use this url to cite or link to this publication:
author
, et al. (More)
(Less)
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Assembly factors, Barrel cortex, Blue native gel electrophoresis, Encephalopathy, Hepatopathy, Microglia, Mitochondrial disorder, Respiratory chain, Respirometry
in
Orphanet Journal of Rare Diseases
volume
12
issue
1
publisher
BioMed Central
external identifiers
  • scopus:85018506430
  • wos:000399829000002
ISSN
1750-1172
DOI
10.1186/s13023-017-0624-2
language
English
LU publication?
yes
id
f958884e-a1cd-4be8-8b50-47ca7fa34e0f
date added to LUP
2017-05-18 12:01:50
date last changed
2017-09-18 11:36:16
@article{f958884e-a1cd-4be8-8b50-47ca7fa34e0f,
  abstract     = {<p>Background: Mitochondrial diseases due to defective respiratory chain complex III (CIII) are relatively uncommon. The assembly of the eleven-subunit CIII is completed by the insertion of the Rieske iron-sulfur protein, a process for which BCS1L protein is indispensable. Mutations in the BCS1L gene constitute the most common diagnosed cause of CIII deficiency, and the phenotypic spectrum arising from mutations in this gene is wide. Results: A case of CIII deficiency was investigated in depth to assess respiratory chain function and assembly, and brain, skeletal muscle and liver histology. Exome sequencing was performed to search for the causative mutation(s). The patient's platelets and muscle mitochondria showed respiration defects and defective assembly of CIII was detected in fibroblast mitochondria. The patient was compound heterozygous for two novel mutations in BCS1L, c.306A &gt; T and c.399delA. In the cerebral cortex a specific pattern of astrogliosis and widespread loss of microglia was observed. Further analysis showed loss of Kupffer cells in the liver. These changes were not found in infants suffering from GRACILE syndrome, the most severe BCS1L-related disorder causing early postnatal mortality, but were partially corroborated in a knock-in mouse model of BCS1L deficiency. Conclusions: We describe two novel compound heterozygous mutations in BCS1L causing CIII deficiency. The pathogenicity of one of the mutations was unexpected and points to the importance of combining next generation sequencing with a biochemical approach when investigating these patients. We further show novel manifestations in brain, skeletal muscle and liver, including abnormality in specialized resident macrophages (microglia and Kupffer cells). These novel phenotypes forward our understanding of CIII deficiencies caused by BCS1L mutations.</p>},
  articleno    = {73},
  author       = {Tegelberg, Saara and Tomašić, Nikica and Kallijärvi, Jukka and Purhonen, Janne and Elmér, Eskil and Lindberg, Eva and Nord, David Gisselsson and Soller, Maria and Lesko, Nicole and Wedell, Anna and Bruhn, Helene and Freyer, Christoph and Stranneheim, Henrik and Wibom, Rolf and Nennesmo, Inger and Wredenberg, Anna and Eklund, Erik A. and Fellman, Vineta},
  issn         = {1750-1172},
  keyword      = {Assembly factors,Barrel cortex,Blue native gel electrophoresis,Encephalopathy,Hepatopathy,Microglia,Mitochondrial disorder,Respiratory chain,Respirometry},
  language     = {eng},
  month        = {04},
  number       = {1},
  publisher    = {BioMed Central},
  series       = {Orphanet Journal of Rare Diseases},
  title        = {Respiratory chain complex III deficiency due to mutated BCS1L : A novel phenotype with encephalomyopathy, partially phenocopied in a Bcs1l mutant mouse model},
  url          = {http://dx.doi.org/10.1186/s13023-017-0624-2},
  volume       = {12},
  year         = {2017},
}