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Prompt epithelial damage and restitution processes in allergen challenged guinea-pig trachea in vivo

Erjefält, Jonas LU ; Korsgren, Magnus LU ; Nilsson, M C ; Sundler, Frank LU and Persson, Carl LU (1997) In Clinical and Experimental Allergy 27(12). p.1458-1470
Abstract
BACKGROUND: Little is known about the induction and the morphology of epithelial damage, and of the ensuing epithelial restitution processes in allergic airways. OBJECTIVE: To examine epithelial damage and restitution in allergen challenged guinea-pig trachea. METHODS: Whole-mount techniques, transmission and scanning electron microscopy, cryosectioning, and histochemical staining were used. Cell proliferation was monitored by BrdU-immunohistochemistry. RESULTS: Allergen challenge produced patchy, crater-like, and leucocyte-rich epithelial damage sites. At 1, 5, and 24 h damage was associated with poorly differentiated epithelial restitution cells. Already at 1 h the epithelial craters had a floor of flattened restitution cells and the... (More)
BACKGROUND: Little is known about the induction and the morphology of epithelial damage, and of the ensuing epithelial restitution processes in allergic airways. OBJECTIVE: To examine epithelial damage and restitution in allergen challenged guinea-pig trachea. METHODS: Whole-mount techniques, transmission and scanning electron microscopy, cryosectioning, and histochemical staining were used. Cell proliferation was monitored by BrdU-immunohistochemistry. RESULTS: Allergen challenge produced patchy, crater-like, and leucocyte-rich epithelial damage sites. At 1, 5, and 24 h damage was associated with poorly differentiated epithelial restitution cells. Already at 1 h the epithelial craters had a floor of flattened restitution cells and the damaged areas comprised < 1% of the mucosal surface area (whole-mount preparations). In contrast, cryo sections displayed large areas (approximately 20%, 1 h) of denudation. Epithelial, and subepithelial (fibroblasts, smooth muscle) proliferation was increased 5 and 24 h after challenge (P < 0.01). CONCLUSION: Within 1 h allergen challenge has induced patchy damage sites where epithelial restitution is already advanced; although easily produced by cryosectioning frank denudation was not evident in whole-mount preparations. The present findings may explain the well maintained, functional tightness of allergic airways displaying epithelial damage, shedding, and even denudation. The present data also suggest the possibility that epithelial damage-restitution may be causative to allergic airway remodelling. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
epithelial restitution, allergic airways, epithelial damage
in
Clinical and Experimental Allergy
volume
27
issue
12
pages
1458 - 1470
publisher
Wiley-Blackwell
external identifiers
  • pmid:9433942
  • scopus:0031466599
ISSN
1365-2222
DOI
10.1046/j.1365-2222.1997.1200932.x
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Airway Inflammation and Immunology (013212038), Division of Clinical Chemistry and Pharmacology (013250300), Neuroendocrine Cell Biology (013212008)
id
f990bbd0-fe82-49fc-9a7d-41695199c9c4 (old id 1112495)
date added to LUP
2016-04-01 12:16:11
date last changed
2020-01-12 09:31:45
@article{f990bbd0-fe82-49fc-9a7d-41695199c9c4,
  abstract     = {BACKGROUND: Little is known about the induction and the morphology of epithelial damage, and of the ensuing epithelial restitution processes in allergic airways. OBJECTIVE: To examine epithelial damage and restitution in allergen challenged guinea-pig trachea. METHODS: Whole-mount techniques, transmission and scanning electron microscopy, cryosectioning, and histochemical staining were used. Cell proliferation was monitored by BrdU-immunohistochemistry. RESULTS: Allergen challenge produced patchy, crater-like, and leucocyte-rich epithelial damage sites. At 1, 5, and 24 h damage was associated with poorly differentiated epithelial restitution cells. Already at 1 h the epithelial craters had a floor of flattened restitution cells and the damaged areas comprised &lt; 1% of the mucosal surface area (whole-mount preparations). In contrast, cryo sections displayed large areas (approximately 20%, 1 h) of denudation. Epithelial, and subepithelial (fibroblasts, smooth muscle) proliferation was increased 5 and 24 h after challenge (P &lt; 0.01). CONCLUSION: Within 1 h allergen challenge has induced patchy damage sites where epithelial restitution is already advanced; although easily produced by cryosectioning frank denudation was not evident in whole-mount preparations. The present findings may explain the well maintained, functional tightness of allergic airways displaying epithelial damage, shedding, and even denudation. The present data also suggest the possibility that epithelial damage-restitution may be causative to allergic airway remodelling.},
  author       = {Erjefält, Jonas and Korsgren, Magnus and Nilsson, M C and Sundler, Frank and Persson, Carl},
  issn         = {1365-2222},
  language     = {eng},
  number       = {12},
  pages        = {1458--1470},
  publisher    = {Wiley-Blackwell},
  series       = {Clinical and Experimental Allergy},
  title        = {Prompt epithelial damage and restitution processes in allergen challenged guinea-pig trachea in vivo},
  url          = {http://dx.doi.org/10.1046/j.1365-2222.1997.1200932.x},
  doi          = {10.1046/j.1365-2222.1997.1200932.x},
  volume       = {27},
  year         = {1997},
}