Putative cholinergic elements in the photosensory pineal organ and retina of a teleost, Phoxinus phoxinus L. (Cyprinidae) - Distribution of choline acetyltransferase immunoreactivity, acetylcholinesterase-positive elements and pinealofugally projecting neurons
(1986) In Cell and Tissue Research 246(2). p.321-329- Abstract
Putative cholinergic neurons in the photosensory pineal organ of a cyprinid teleost, the European minnow, were studied by use of choline acetyltransferase (ChAT) immunocytochemistry and acetylcholinesterase (AChE) histochemistry. Pinealofugally projecting neurons were visualized using retrograde HRP-filling through their cut axons. For comparison, the distribution of choline acetyltransferase immunoreactivity (ChAT-IR) and AChE-positive elements in the retina was investigated. While the distributional patterns of ChAT-IR and strongly AChE-positive perikarya in the retina are similar and may represent the same neuronal population, ChAT-IR and AChE-positive elements in the pineal organ appear to belong to separate populations. In the... (More)
Putative cholinergic neurons in the photosensory pineal organ of a cyprinid teleost, the European minnow, were studied by use of choline acetyltransferase (ChAT) immunocytochemistry and acetylcholinesterase (AChE) histochemistry. Pinealofugally projecting neurons were visualized using retrograde HRP-filling through their cut axons. For comparison, the distribution of choline acetyltransferase immunoreactivity (ChAT-IR) and AChE-positive elements in the retina was investigated. While the distributional patterns of ChAT-IR and strongly AChE-positive perikarya in the retina are similar and may represent the same neuronal population, ChAT-IR and AChE-positive elements in the pineal organ appear to belong to separate populations. In the retina, small- to medium-sized perikarya in the inner nuclear layer, and small perikarya in the ganglion cell layer are ChAT-IR and AChE positive. The entire inner plexiform layer is AChE positive, while only sublaminae 1, 2 and 4 are ChAT-IR. No indication of cholinergic activity was observed in the optic axon layer. In the pineal organ, ChAT-IR is restricted to small perikarya situated rostrally and dorsally in the pineal end-vesicle. AChE-positive neurons are present throughout the pineal end-vesicle and the pineal stalk. The pineal tract (the pinealofugally projecting axons of intrapineal neurons) is strongly AChE positive, but displays no ChAT-IR. The distribution of pinealofugally projecting neurons, labeled with retrogradely transported HRP, is markedly dissimilar to that of the ChAT-IR elements. It is proposed that the photosensory pineal organ transmits photic information to the brain via a non-cholinergic pathway. The possibility that the ChAT-IR neurons represent small local interneurons is discussed in the light of comparative physiological and anatomical findings.
(Less)
- author
- Ekström, Peter LU and Korf, Horst W.
- organization
- publishing date
- 1986-11-01
- type
- Contribution to journal
- publication status
- published
- keywords
- Acetylcholinesterase, Choline acetyltransferase, Horseradish-peroxidase (HRP), transport, Photoreceptors, extraocular, Phoxinus hoxinus (Teleostei, Cyprinidae), Pineal organ, Retina
- in
- Cell and Tissue Research
- volume
- 246
- issue
- 2
- pages
- 9 pages
- publisher
- Springer
- external identifiers
-
- scopus:0022922711
- pmid:3779811
- ISSN
- 0302-766X
- DOI
- 10.1007/BF00215894
- language
- English
- LU publication?
- yes
- id
- f9f4bbf2-76c9-4f52-8d17-49bf42f88fde
- date added to LUP
- 2019-10-03 10:27:57
- date last changed
- 2024-01-01 21:50:45
@article{f9f4bbf2-76c9-4f52-8d17-49bf42f88fde,
abstract = {{<p>Putative cholinergic neurons in the photosensory pineal organ of a cyprinid teleost, the European minnow, were studied by use of choline acetyltransferase (ChAT) immunocytochemistry and acetylcholinesterase (AChE) histochemistry. Pinealofugally projecting neurons were visualized using retrograde HRP-filling through their cut axons. For comparison, the distribution of choline acetyltransferase immunoreactivity (ChAT-IR) and AChE-positive elements in the retina was investigated. While the distributional patterns of ChAT-IR and strongly AChE-positive perikarya in the retina are similar and may represent the same neuronal population, ChAT-IR and AChE-positive elements in the pineal organ appear to belong to separate populations. In the retina, small- to medium-sized perikarya in the inner nuclear layer, and small perikarya in the ganglion cell layer are ChAT-IR and AChE positive. The entire inner plexiform layer is AChE positive, while only sublaminae 1, 2 and 4 are ChAT-IR. No indication of cholinergic activity was observed in the optic axon layer. In the pineal organ, ChAT-IR is restricted to small perikarya situated rostrally and dorsally in the pineal end-vesicle. AChE-positive neurons are present throughout the pineal end-vesicle and the pineal stalk. The pineal tract (the pinealofugally projecting axons of intrapineal neurons) is strongly AChE positive, but displays no ChAT-IR. The distribution of pinealofugally projecting neurons, labeled with retrogradely transported HRP, is markedly dissimilar to that of the ChAT-IR elements. It is proposed that the photosensory pineal organ transmits photic information to the brain via a non-cholinergic pathway. The possibility that the ChAT-IR neurons represent small local interneurons is discussed in the light of comparative physiological and anatomical findings.</p>}},
author = {{Ekström, Peter and Korf, Horst W.}},
issn = {{0302-766X}},
keywords = {{Acetylcholinesterase; Choline acetyltransferase; Horseradish-peroxidase (HRP), transport; Photoreceptors, extraocular; Phoxinus hoxinus (Teleostei, Cyprinidae); Pineal organ; Retina}},
language = {{eng}},
month = {{11}},
number = {{2}},
pages = {{321--329}},
publisher = {{Springer}},
series = {{Cell and Tissue Research}},
title = {{Putative cholinergic elements in the photosensory pineal organ and retina of a teleost, Phoxinus phoxinus L. (Cyprinidae) - Distribution of choline acetyltransferase immunoreactivity, acetylcholinesterase-positive elements and pinealofugally projecting neurons}},
url = {{http://dx.doi.org/10.1007/BF00215894}},
doi = {{10.1007/BF00215894}},
volume = {{246}},
year = {{1986}},
}