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Method for the biological monitoring of hexahydrophthalic anhydride by the determination of hexahydrophthalic acid in urine using gas chromatography and selected-ion monitoring

Jönsson, Bo A LU and Skarping, Gunnar LU (1991) In Journal of Chromatography A 572(1-2). p.117-131
Abstract
A method for the determination of hexahydrophthalic acid, a metabolite of hexahydrophthalic anhydride, in human urine has been developed. The urine was worked-up by liquid-solid extraction, esterified with boron trifluoride-methanol, and analysed by capillary gas chromatography and selected-ion monitoring. Hexadeuterium-labelled hexahydrophthalic acid was used as the internal standard. The precision was 4% at 0.7 microgram/ml and 5% at 0.07 microgram/ml. The recovery of the acid for the overall method was 101% at 0.07 micrograms/ml of urine (with a coefficient of variation of 4%) and 95% at 0.7 microgram/ml (coefficient of variation 2%). The limit of detection was 20 ng/ml urine.
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author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Chromatography A
volume
572
issue
1-2
pages
117 - 131
publisher
Elsevier
external identifiers
  • pmid:1818048
  • scopus:0026323853
ISSN
0021-9673
language
English
LU publication?
yes
id
fa172fcb-dec8-4188-9a6f-7864c79ddbff (old id 1105704)
date added to LUP
2016-04-01 16:52:28
date last changed
2021-01-03 11:31:33
@article{fa172fcb-dec8-4188-9a6f-7864c79ddbff,
  abstract     = {{A method for the determination of hexahydrophthalic acid, a metabolite of hexahydrophthalic anhydride, in human urine has been developed. The urine was worked-up by liquid-solid extraction, esterified with boron trifluoride-methanol, and analysed by capillary gas chromatography and selected-ion monitoring. Hexadeuterium-labelled hexahydrophthalic acid was used as the internal standard. The precision was 4% at 0.7 microgram/ml and 5% at 0.07 microgram/ml. The recovery of the acid for the overall method was 101% at 0.07 micrograms/ml of urine (with a coefficient of variation of 4%) and 95% at 0.7 microgram/ml (coefficient of variation 2%). The limit of detection was 20 ng/ml urine.}},
  author       = {{Jönsson, Bo A and Skarping, Gunnar}},
  issn         = {{0021-9673}},
  language     = {{eng}},
  number       = {{1-2}},
  pages        = {{117--131}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Chromatography A}},
  title        = {{Method for the biological monitoring of hexahydrophthalic anhydride by the determination of hexahydrophthalic acid in urine using gas chromatography and selected-ion monitoring}},
  volume       = {{572}},
  year         = {{1991}},
}