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Baring it all- undressing Cambrian ´Orsten´phosphatocopine arthropods using synchrotron X-ray tomographic microscopy

Eriksson, Mats LU ; Terfelt, Fredrik LU ; Elofsson, Rolf LU ; Maas, Andreas; Marone, Federica; Lindskog, Anders LU ; Waloszek, Dieter; Schmitz, Birger LU and Stampioni, Marco (2016) In Lethaia 49(3). p.312-326
Abstract
Synchrotron radiation X-ray tomographic microscopy (SRXTM) was used to virtually dissect and peel the shields off of the microscopic, bivalved phosphatocopine crustaceans in the Cambrian ‘Orsten’ type of preservation of Sweden. Doing so opened up for an array of concealed internal structures to be observed in a fully enclosed specimen of Hesslandona ventrospinata and a semi-enclosed specimen of Hesslandona angustata. For comparison, also a head-larva stage specimen of H. angustata, with shields in ‘butterfly position’, was analysed. The X-ray tomographic data sets revealed excellently preserved structures, such as labrum, sternum, antennae, mandibular and post-mandibular limbs with their minute setae, all of which were more or less... (More)
Synchrotron radiation X-ray tomographic microscopy (SRXTM) was used to virtually dissect and peel the shields off of the microscopic, bivalved phosphatocopine crustaceans in the Cambrian ‘Orsten’ type of preservation of Sweden. Doing so opened up for an array of concealed internal structures to be observed in a fully enclosed specimen of Hesslandona ventrospinata and a semi-enclosed specimen of Hesslandona angustata. For comparison, also a head-larva stage specimen of H. angustata, with shields in ‘butterfly position’, was analysed. The X-ray tomographic data sets revealed excellently preserved structures, such as labrum, sternum, antennae, mandibular and post-mandibular limbs with their minute setae, all of which were more or less dis-
guised by the enclosing shields. This, moreover, allowed assignment to growth stages of the specimens, which is impossible based solely on external morphology and size.
Microspherules observed inside the shields of the semi-enclosed H. angustata specimen may represent remains of food particles, and the feeding biology of phosphatocopines is discussed in detail. Our analyses suggest that phosphatocopines were particle feeders. The SRXTM technique offers the ability to three-dimensionally reconstruct the morphology in high resolution, construct virtual serial sections and study concealed structures. The resulting data allow for new structures to be revealed for previously known taxa and for new taxa to be identified, with the added benefit of not destroying the specimens in the process. Hence, we do not longer have to rely on serendipitous finds of broken and/or open phosphatocopine specimens to elucidate their diagnostic ventral morphology.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
feeding biology, Phosphatocopina, SRXTM, ventral morphology
in
Lethaia
volume
49
issue
3
pages
312 - 326
publisher
Taylor & Francis
external identifiers
  • scopus:84944395828
  • wos:000384100300004
ISSN
0024-1164
DOI
10.1111/let.12149
language
English
LU publication?
yes
id
fa3fe944-36d9-46a5-8881-9da905261d6d
date added to LUP
2016-10-28 22:03:11
date last changed
2017-10-22 05:21:12
@article{fa3fe944-36d9-46a5-8881-9da905261d6d,
  abstract     = {Synchrotron radiation X-ray tomographic microscopy (SRXTM) was used to virtually dissect and peel the shields off of the microscopic, bivalved phosphatocopine crustaceans in the Cambrian ‘Orsten’ type of preservation of Sweden. Doing so opened up for an array of concealed internal structures to be observed in a fully enclosed specimen of Hesslandona ventrospinata and a semi-enclosed specimen of Hesslandona angustata. For comparison, also a head-larva stage specimen of H. angustata, with shields in ‘butterfly position’, was analysed. The X-ray tomographic data sets revealed excellently preserved structures, such as labrum, sternum, antennae, mandibular and post-mandibular limbs with their minute setae, all of which were more or less dis-<br/>guised by the enclosing shields. This, moreover, allowed assignment to growth stages of the specimens, which is impossible based solely on external morphology and size.<br/>Microspherules observed inside the shields of the semi-enclosed H. angustata specimen may represent remains of food particles, and the feeding biology of phosphatocopines is discussed in detail. Our analyses suggest that phosphatocopines were particle feeders. The SRXTM technique offers the ability to three-dimensionally reconstruct the morphology in high resolution, construct virtual serial sections and study concealed structures. The resulting data allow for new structures to be revealed for previously known taxa and for new taxa to be identified, with the added benefit of not destroying the specimens in the process. Hence, we do not longer have to rely on serendipitous finds of broken and/or open phosphatocopine specimens to elucidate their diagnostic ventral morphology.<br/>},
  author       = {Eriksson, Mats and Terfelt, Fredrik and Elofsson, Rolf and Maas, Andreas and Marone, Federica and Lindskog, Anders and Waloszek, Dieter and Schmitz, Birger and Stampioni, Marco},
  issn         = {0024-1164},
  keyword      = {feeding biology,Phosphatocopina,SRXTM,ventral morphology},
  language     = {eng},
  number       = {3},
  pages        = {312--326},
  publisher    = {Taylor & Francis},
  series       = {Lethaia},
  title        = {Baring it all- undressing Cambrian ´Orsten´phosphatocopine arthropods using synchrotron X-ray tomographic microscopy},
  url          = {http://dx.doi.org/10.1111/let.12149},
  volume       = {49},
  year         = {2016},
}